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Study on the role of nuclear glycosylated actin in the diabetic complications

Research Project

Project/Area Number 18K06840
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 48010:Anatomy-related
Research InstitutionKyorin University

Principal Investigator

Akimoto Yoshihiro  杏林大学, 医学部, 教授 (60184115)

Co-Investigator(Kenkyū-buntansha) 宮東 昭彦  杏林大学, 医学部, 准教授 (80255398)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords糖尿病合併症 / 糖修飾アクチン / O-GlcNAc / リン酸化 / 腎臓 / 網膜 / 坐骨神経 / 糖尿病 / アクチン / O-GlcNAc化 / 腎症 / OGT / OGA / GKラット / 糖修飾 / 核内 / 核
Outline of Final Research Achievements

Antibody against glycosylated (O-GlcNAc-modified) actin was prepared and immunostaining was performed to compare the localization in tissues of normal and diabetic rat and human. It was shown that the immunostaining intensity both in the nucleus and cytoplasm increased in the diabetic tissues.
Furthermore, in order to investigate the nuclear protein that interacts with glycosylated actin, immunoprecipitation and mass spectrometry were performed on the nuclear fraction proteins of normal and diabetic rat kidneys, and proteins that were particularly increased in diabetes were identified.

Academic Significance and Societal Importance of the Research Achievements

糖修飾アクチンが核内でどのような役割を担っているかについての研究は、国内外を通じていまだ行われていない。本研究は、従来、糖尿病性合併症を起こすとして考えられていた病因に加えて、さらに核内アクチンの糖修飾(O-GlcNAc化)という観点から検討するところが独創的な点である。新たな病因の分子機序の解明に寄与すると考えられる。また、核内アクチンのO-GlcNAc化の程度を測定し、その亢進を抑制することができれば糖尿病合併症の診断、予防や治療に向けた基礎的知見として役立つことが期待される。本研究の進展により、将来的には糖尿病合併症の新しい治療法開発へと応用されることが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (33 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 12 results,  Open Access: 5 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (5 results) Remarks (1 results)

  • [Int'l Joint Research] University of Georgia(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] A novel podocyte protein, R3h domain containing-like, inhibits TGF-β-induced p38 MAPK and regulates the structure of podocytes and glomerular basement membrane2021

    • Author(s)
      Ishikawa Takahiro、Takemoto Minoru、Akimoto Yoshihiro、Takada-Watanabe Aki、Yan Kunimasa、Sakamoto Kenichi、Maezawa Yoshiro、Suguro Miyuki、He Liqun、Tryggvason Karl、Betsholtz Christer、Yokote Koutaro
    • Journal Title

      Journal of Molecular Medicine

      Volume: 6 Issue: 6 Pages: 859-876

    • DOI

      10.1007/s00109-021-02050-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Growth inhibition effect on Trypanosoma brucei gambiense by the oxidative stress supplied from low-temperature plasma at atmospheric pressure2021

    • Author(s)
      Yokoyama Naoaki、Sivakumar Thillaiampalam、Ikehara Sanae、Akimoto Yoshihiro、Yamaguchi Takashi、Wakai Ken、Ishikawa Kenji、Hori Masaru、Shimizu Tetsuji、Sakakita Hajime、Ikehara Yuzuru
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 60 Issue: 2 Pages: 020601-020601

    • DOI

      10.35848/1347-4065/abd464

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mucin-type O-glycosylation controls pluripotency in mouse embryonic stem cells via Wnt receptor endocytosis2020

    • Author(s)
      Pecori Federico、Akimoto Yoshihiro、Hanamatsu Hisatoshi、Furukawa Jun-ichi、Shinohara Yasuro、Ikehara Yuzuru、Nishihara Shoko
    • Journal Title

      Journal of Cell Science

      Volume: 133 Issue: 20 Pages: 245845-245845

    • DOI

      10.1242/jcs.245845

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Toxicity of Gold Nanoparticles in Mice due to Nanoparticle/Drug Interaction Induces Acute Kidney Damage2020

    • Author(s)
      Isoda Katsuhiro、Tanaka Anju、Fuzimori Chisaki、Echigoya Miyuki、Taira Yuichiro、Taira Ikuko、Shimizu Yoshimi、Akimoto Yoshihiro、Kawakami Hayato、Ishida Isao
    • Journal Title

      Nanoscale Research Letters

      Volume: 15 Issue: 1 Pages: 141-150

    • DOI

      10.1186/s11671-020-03371-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stress2020

    • Author(s)
      Fujihira Haruhiko、Masahara-Negishi Yuki、Akimoto Yoshihiro、Hirayama Hiroto、Lee Hyeon-Cheol、Story Benjamin A.、Mueller William F.、Jakob Petra、Clauder-M?nster Sandra、Steinmetz Lars M.、Radhakrishnan Senthil K.、Kawakami Hayato、Kamada Yoshihiro、Miyoshi Eiji、Yokomizo Takehiko、Suzuki Tadashi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

      Volume: 1866 Issue: 3 Pages: 165588-165588

    • DOI

      10.1016/j.bbadis.2019.165588

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] O-GlcNAcylation and phosphorylation of β-actin Ser199 in diabetic nephropathy2019

    • Author(s)
      Akimoto Yoshihiro、Yan Kunimasa、Miura Yuri、Tsumoto Hiroki、Toda Tosifusa、Fukutomi Toshiyuki、Sugahara Daisuke、Kudo Akihiko、Arai Tomio、Chiba Yuko、Kaname Shinya、Hart Gerald W.、Endo Tamao、Kawakami Hayato
    • Journal Title

      American Journal of Physiology-Renal Physiology

      Volume: 317 Issue: 5 Pages: F1359-F1374

    • DOI

      10.1152/ajprenal.00566.2018

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fumarate accumulation involved in renal diabetic fibrosis in Goto-Kakizaki rats2019

    • Author(s)
      Miura Yuri、Hayakawa Atsuko、Kikuchi Shohei、Tsumoto Hiroki、Umezawa Keitaro、Chiba Yuko、Soejima Yurie、Sawabe Motoji、Fukui Koji、Akimoto Yoshihiro、Endo Tamao
    • Journal Title

      Archives of Biochemistry and Biophysics

      Volume: 678 Pages: 108167-108167

    • DOI

      10.1016/j.abb.2019.108167

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] TGF-β signaling promotes tube-structure-forming growth in pancreatic duct adenocarcinoma2019

    • Author(s)
      Yamaguchi Takashi、Ikehara Sanae、Akimoto Yoshihiro、Nakanishi Hayao、Kume Masahiko、Yamamoto Kazuo、Ohara Osamu、Ikehara Yuzuru
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 11247-11247

    • DOI

      10.1038/s41598-019-47101-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] R3hdml regulates satellite cell proliferation and differentiation2019

    • Author(s)
      Sakamoto Kenichi、Furuichi Yasuro、Yamamoto Masashi、Takahashi Megumi、Akimoto Yoshihiro、Ishikawa Takahiro、Shimizu Takahiko、Fujimoto Masanori、Takada‐Watanabe Aki、Hayashi Aiko、Mita Yoshitaka、Manabe Yasuko、Fujii Nobuharu L、Ishibashi Ryoichi、Maezawa Yoshiro、Betsholtz Christer、Yokote Koutaro、Takemoto Minoru
    • Journal Title

      EMBO reports

      Volume: 20 Issue: 11 Pages: 1-12

    • DOI

      10.15252/embr.201947957

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Systematic diagnostics of the electrical, optical, and physicochemical characteristics of low-temperature atmospheric-pressure helium plasma sources.2019

    • Author(s)
      Keigo Takeda, Hiromasa Yamada, Kenji Ishikawa, Hajime Sakakita, Jaeho Kim, Masashi Ueda, Jun-ichiro Ikeda, Yoshihiro Akimoto, Yosky Kataoka, Naoaki Yokoyama, Yuzuru Ikehara, Masaru Hori
    • Journal Title

      J Phys D: Appl Phys

      Volume: 52 Pages: 165202-165202

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcription Factor 21 Is Required for Branching Morphogenesis and Regulates the Gdnf-Axis in Kidney Development2018

    • Author(s)
      Ide S, Finer G, Maezawa Y, Onay T, Souma T, Scott R, Ide K, Akimoto Y, Li C,Ye M, Zhao X, Baba Y, Minamizuka T, Jin J, Takemoto M, Yokote K, Quaggin SE
    • Journal Title

      J Am Soc Nephrol

      Volume: 29 Issue: 12 Pages: 2795-2808

    • DOI

      10.1681/asn.2017121278

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles.2018

    • Author(s)
      Itoh K, Akimoto Y, Kondo S, Ichimiya T, Aoki K, Tiemeyer M, Nishihara S
    • Journal Title

      Develop Biol

      Volume: 436 Issue: 2 Pages: 108-124

    • DOI

      10.1016/j.ydbio.2018.02.017

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 電顕チュートリアル 様々な試料作製法「細胞の構造と機能」2021

    • Author(s)
      秋元義弘
    • Organizer
      日本顕微鏡学会第44回関東支部実技講習会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 糖修飾・リン酸化ベータ アクチンの核内における役割2021

    • Author(s)
      秋元義弘,三浦ゆり,宮東昭彦,Hart GW,遠藤玉夫
    • Organizer
      第126回日本解剖学会総会・全国学術集会/第98回日本生理学会大会 合同大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Mucin-type O-glycosylation regulates pluripotency in mouse embryonic stem cells via Wnt receptor internalization.2020

    • Author(s)
      Pecori F, Akimoto Y, Hanamatsu H, Furukawa J, Nishihara S
    • Organizer
      International Society for Stem Cell Research (ISSCR) 2020 Annual Meeting
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 免疫電子顕微鏡法の基礎と応用2020

    • Author(s)
      秋元義弘,宮東昭彦
    • Organizer
      第45回組織細胞化学講習会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大気圧非平衡プラズマを用いた糖鎖機能理解の深化とその利用2020

    • Author(s)
      池原早苗,秋元義弘,山口高志,榊田創,堀勝,池原譲
    • Organizer
      第81回応用物理学会秋季学術講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] T antigen regulates pluripotency in mouse embryonic stem cells by directly modulating galectin-3 mediated frizzled-5 endocytosis2020

    • Author(s)
      Pecori F, Akimoto Y, Hanamatsu H, Furukawa J, Shinohara Y, Ikehara Y, Nishihara S
    • Organizer
      Annual Meeting of The Society for Glycobiology
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] α1,2 フコシル化糖鎖の発現分布はマウス大腸長軸方向において異なる2020

    • Author(s)
      菅原大介,秋元義弘,川上速人
    • Organizer
      第39回日本糖質学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Frizzled-5のT抗原は、マウス胚性幹細胞の多能性を調節する2020

    • Author(s)
      ペーコリ・フェデリーコ,秋元義弘,花松久寿,古川潤一,篠原康郎,池原譲,西原祥子
    • Organizer
      第39回日本糖質学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Galectin-3 binding to t antigen directly modulates Wnt signaling via frizzled-5 endocytosis in mouse embryonic stem cells2020

    • Author(s)
      Pecori F, Akimoto Y, Hanamatsu H, Furukawa J, Shinohara Y, Ikehara Y, Nishihara S
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 糖尿病性腎症における糖修飾アクチンの免疫組織化学的解析2020

    • Author(s)
      秋元義弘,楊國昌,三浦ゆり,津元裕樹,戸田年総,福冨俊之,菅原大介,宮東昭彦,新井冨生,千葉優子,要伸也,Hart GW,遠藤玉夫,川上速人
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Histochemical analysis of galactose-binding lectin galectin: galectin expression in healing wounded skin treated with low-temperature plasma.2019

    • Author(s)
      Yoshihiro Akimoto
    • Organizer
      China-Japan High-end Forum on Medical and Health Cooperation.
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 糖修飾(O-GlcNAc化)と糖尿病性腎症2019

    • Author(s)
      秋元義弘
    • Organizer
      第47回 腎研究会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 糖尿病モデルGKラットの坐骨神経、網膜における糖修飾アクチンの局在2019

    • Author(s)
      秋元義弘、三浦ゆり、福冨俊之、菅原大介、宮東昭彦、Gerald W Hart、遠藤玉夫、楊國昌、川上速人
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] ラット腎臓におけるリン酸化アクチンと糖修飾アクチンの局在2018

    • Author(s)
      秋元義弘, 楊 國昌, 三浦ゆり, 津元裕樹, 岩本真知子, 戸田年総, 福冨俊之, 菅原大介, 宮東昭彦,Hart Gerald W, 川上速人
    • Organizer
      第59回日本組織細胞化学会総会・学術集会
    • Related Report
      2018 Research-status Report
  • [Book] Comprehensive Glycoscience, 2nd Edition2021

    • Author(s)
      Yoshihiro Akimoto, Yuri Miura, Tamao Endo, Gerald W Hart
    • Total Pages
      3355
    • Publisher
      Elsevier
    • ISBN
      9780128194751
    • Related Report
      2020 Annual Research Report
  • [Book] 組織細胞化学20202020

    • Author(s)
      秋元義弘,宮東昭彦
    • Total Pages
      250
    • Publisher
      学際企画
    • ISBN
      4906514952
    • Related Report
      2020 Annual Research Report
  • [Book] Glycoscience: Basic Science to Applications2019

    • Author(s)
      Ohtsubo K, Kizuka Y, Taniguchi N, Takahashi M, Yanagisawa K, Kitazume S, Furukawa K, Ohmi Y, Furukawa K, Akimoto Y
    • Total Pages
      368
    • Publisher
      Springer
    • ISBN
      9789811358555
    • Related Report
      2019 Research-status Report
  • [Book] 組織細胞化学20192019

    • Author(s)
      秋元義弘
    • Total Pages
      233
    • Publisher
      学際企画
    • ISBN
      9784906514946
    • Related Report
      2019 Research-status Report
  • [Book] Plasma Medical Science2018

    • Author(s)
      Akimoto Y, Ikehara S, Yamaguchi T, Kim J, Kawakami H, Shimizu N, Hori M, Sakakita H, Ikehara Y
    • Total Pages
      458
    • Publisher
      Academic Press
    • ISBN
      9780128150054
    • Related Report
      2018 Research-status Report
  • [Remarks] 顕微解剖学教室ホームページ

    • URL

      http://www.kyorin-u.ac.jp/univ/user/medicine/anat2/

    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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