Modulation of cancer cells by core fucose-specific antibody

• Project/Area Number: 18K06914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Osaka International Cancer Institute
• Principal Investigator: Taniguchi Naoyuki 地方独立行政法人大阪府立病院機構大阪国際がんセンター(研究所), その他部局等, 所長兼糖鎖オンコロジー部部長 (90002188)
• Co-Investigator(Kenkyū-buntansha): 木塚 康彦 岐阜大学, 高等研究院, 准教授 (20564743) ; 顧 建国 東北医科薬科大学, 薬学部, 教授 (40260369)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: コアフコース / IgG / がん / 糖鎖

Outline of Final Research Achievements

This was research about core fucose glycan of immunoglobulin G (IgG) in human sera. We assayed the level of core fucose of IgG in the sera of lung cancer, chronic obstructive pulmonary disease (COPD), interstitial pneumonia (IP) patients, and control healthy donors and found a low level of it in lung cancer and IP patients. Additionally, we also found that lung cancer cells decreased the expression of core fucose glycan of IgG in the experiments using culture cells.

Academic Significance and Societal Importance of the Research Achievements

今回初めて、肺癌および間質性肺炎患者の免疫グロブリンG上のコアフコース糖鎖の発現が低下していることが明らかとなった。本研究で開発した測定システムを用いれば、血液から肺癌や間質性肺炎を診断できる可能性がある。また、今後、その発現が低下する分子メカニズムが明らかになれば、免疫グロブリンG上のコアフコース糖鎖を標的とした新たな治療法が開発されることが期待される。

Research Products

• [Journal Article] Generation of the heterogeneity of extracellular vesicles by membrane organization and sorting machineries (2019)
  • Author(s): Harada Yoichiro、Suzuki Takehiro、Fukushige Tomoko、Kizuka Yasuhiko、Yagi Hirokazu、Yamamoto Mika、Kondo Kiyotaka、Inoue Hiromasa、Kato Koichi、Taniguchi Naoyuki、Kanekura Takuro、Dohmae Naoshi、Maruyama Ikuro
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1863 Issue: 4 Pages: 681-691
  • DOI: 10.1016/j.bbagen.2019.01.015
  • Peer Reviewed
• [Journal Article] Implication of C-type lectin receptor langerin and keratan sulfate disaccharide in emphysema (2018)
  • Author(s): Kizuka Yasuhiko、Mishra Sushil、Yamaguchi Yoshiki、Taniguchi Naoyuki
  • Journal Title: Cellular Immunology Volume: 333 Pages: 80-84
  • DOI: 10.1016/j.cellimm.2018.07.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Neural functions of bisecting GlcNAc. (2018)
  • Author(s): Kizuka Y, Taniguchi N
  • Journal Title: Glycoconj. J. Volume: ー Issue: 4 Pages: 345-351
  • DOI: 10.1007/s10719-018-9829-4
  • Peer Reviewed
• [Journal Article] High affinity sugar ligands of C-type lectin receptor langerin. (2018)
  • Author(s): Ota F, Hirayama T, Kizuka Y, Yamaguchi Y, Fujinawa R, Nagata M; Ismanto HS, Lepenies, Aretz J, Rademacher C, Seeberger PH; Angata T, Kitazume S, Yoshida K, Betsuyaku T, Kida K Yamasaki S, and Taniguchi N
  • Journal Title: BBA - General Subjects Volume: - Issue: 7 Pages: 1592-1601
  • DOI: 10.1016/j.bbagen.2018.04.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 1.Imamaki R. Ogawa K, Kizuka Y, Komi Y, Kojima S, Kotani N, Honke K, Honda T, Taniguchi N, and Kitazume S (2018)
  • Author(s): 1.Imamaki R. Ogawa K, Kizuka Y, Komi Y, Kojima S, Kotani N, Honke K, Honda T, Taniguchi N, and Kitazume S
  • Journal Title: Oncogene Volume: - Issue: 31 Pages: 4287-4299
  • DOI: 10.1038/s41388-018-0271-7
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic control of T cell immune response through glycans in inflammatory bowel disease. (2018)
  • Author(s): Dias AM, Correia A, Pereira MS, Almeida CR, Alves I, Pinto V, Catarino TA, Mendes S, Leander M, Oliva-Teles MT, Maia L, Delerue-Matos C, Taniguchi N, Lima M, Pedroto I, Marcos-Pinto R, Lago P, Reis CA, Vilanova M, Pinho SS
  • Journal Title: Proc Natl Acad Sci U S A Volume: 115(20) Issue: 20
  • DOI: 10.1073/pnas.1720409115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V (2018)
  • Author(s): Nagae Masamichi、Kizuka Yasuhiko、Mihara Emiko、Kitago Yu、Hanashima Shinya、Ito Yukishige、Takagi Junichi、Taniguchi Naoyuki、Yamaguchi Yoshiki
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 3380-3380
  • DOI: 10.1038/s41467-018-05931-w
  • Peer Reviewed / Open Access
• [Journal Article] Core fucose is essential glycosylation for CD14-dependent Toll-like receptor 4 and Toll-like receptor 2 signaling in macrophages (2018)
  • Author(s): Nakayama K, Wakamatsu K, Fujii H, Shinzaki S, Takamatsu S, Kitazume S, Kamada Y, Takehara T, Taniguchi N, Miyoshi E
  • Journal Title: J Biochem Volume: ー Issue: 3 Pages: 227-237
  • DOI: 10.1093/jb/mvy098
  • NAID: 40021845862
  • Peer Reviewed
• [Journal Article] Core fucosylation of the T cell receptor is required for T cell activation. (2018)
  • Author(s): 8.Liang W., Mao S., Sun S., Li M., Li Z., Yu R., Ma T., Gu J., Zhang J., Taniguchi N., Li W.
  • Journal Title: Front Immunol., Volume: 9 Pages: 78-78
  • DOI: 10.3389/fimmu.2018.00078
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs (2018)
  • Author(s): Nakajima Kazuki、Kizuka Yasuhiko、Yamaguchi Yoshiki、Hirabayashi Yoshio、Takahashi Kazuo、Yuzawa Yukio、Taniguchi Naoyuki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 1 Pages: 401-407
  • DOI: 10.1016/j.bbrc.2017.10.173
  • Peer Reviewed / Int'l Joint Research
• [Presentation] グルタチオン研究からシステム糖鎖生物学へ Old sake in a new bottle (2018)
  • Author(s): 谷口 直之
  • Organizer: 日本NO学会日本酸化ストレス学会
• [Presentation] 糖鎖研究の目指すもの (2018)
  • Author(s): 谷口 直之
  • Organizer: 第65回日本生化学会近畿支部例会
• [Presentation] Systems Glycobiology in Disease and N-glycan Branching. (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: 24th IUBMB Congress & 15th FAOBMB Congress 2018
  • Int'l Joint Research
• [Presentation] Branching N-glycans in disease: biomarker discovery and therapeutic approach (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: The 11th International Symposium on Glycosyltransferases (GlycoT 2018)
  • Int'l Joint Research
• [Presentation] Glycosyltransferases: Structure and Function I (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: The 11th International Symposium on Glycosyltransferases (GlycoT 2018)
  • Int'l Joint Research
• [Presentation] 糖鎖研究の目指すもの (2018)
  • Author(s): 谷口 直之
  • Organizer: オリエンタル酵母長浜研究所 研究会
• [Presentation] A unique N-glycan structure, bisecting GlcNAc and Alzheimer’s disease (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: 15th Meeting of the Asian-Pacific Society for Neurochemistry
  • Int'l Joint Research
• [Presentation] Sugar chains (Glycans) and Cancer (2018)
  • Author(s): 谷口 直之
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 糖鎖と病気の発症、診断、治療 (2018)
  • Author(s): 谷口 直之
  • Organizer: 山口大学医学部 講義
• [Presentation] コレクチンと補体系について (2018)
  • Author(s): 谷口 直之
  • Organizer: 第28回日本メイラード学会年会
• [Presentation] Outline of Two-Year Strategic Plan for the HPP (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: HUPO 2018
  • Int'l Joint Research
• [Presentation] Nutrient Regulation of Signaling and Gene Expression by O-GlcNAc (2018)
  • Author(s): 谷口 直之
  • Organizer: 第16回糖鎖科学コンソーシアムシンポジウム
• [Presentation] Branching N-glycans and diseases: approach from biochemistry to systems glycobiology (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: 2018 Annual Meeting of the Society for Glycobiology
  • Int'l Joint Research
• [Presentation] Branching N-glycans in disease onset, biomarker discovery and therapeutics (2018)
  • Author(s): Naoyuki Taniguchi
  • Organizer: 1st Academic Seminar of IPRC & IGRC
  • Int'l Joint Research / Invited
• [Book] Glycoscience: Basic Science to Applications - Insights from the Japan Consortium for Glycobiology and Glycotechnology - (2019)
  • Author(s): Taniguchi N, Endo T, Hirabayashi J, Nishihara S, Kadomatsu K, Akiyoshi K, Aoki-Kinoshita KF, eds.
  • Total Pages: 337
  • Publisher: Springer Singapore
  • ISBN: 9789811358555
• [Book] ベインズ・ドミニチャク生化学（原書4版） (2018)
  • Author(s): Baynes John W., Dominiczak Marek H, 谷口 直之, 岩井 一宏, 藤井 順逸, 本家 孝一 (担当:共著)
  • Total Pages: 728
  • Publisher: エルゼビア・ジャパン, 丸善出版 (発売)
  • ISBN: 9784621301692