Functional analysis of anorexic N-acylethanolamine

• Project/Area Number: 18K06915
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Kagawa University
• Principal Investigator: Uyama Toru 香川大学, 医学部, 助教 (30457337)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: cPLA2ε / PLA2G4E / N-アシル-PE / N-アシルエタノールアミン / N-アシル-ホスファチジルエタノールアミン / 脂質代謝酵素 / カルシウム / ホスファチジルセリン / 脂質メディエーター / Ca-NAT / PLAATファミリー

Outline of Final Research Achievements

N-Acylphosphatidylethanolamine (N-acyl-PE) is a phospholipid present in biomembrane and functions as a precursor of bioactive N-acylethanolamines. Recently, the ε isoform of cytosolic phospholipase A2 (cPLA2ε, also known as PLA2G4E) was identified as Ca2+-dependent N-acyltransferase. In this study, we analyzed biological roles of cPLA2ε.

Academic Significance and Societal Importance of the Research Achievements

N-アシルエタノールアミンは食欲抑制や抗炎症作用などの多様な生物活性を示す。N-アシルエタノールアミンがどのように合成されるかを理解することで、食欲や炎症などを調節することが可能になるかもしれない。

Research Products

• [Journal Article] Functions and biosynthetic mechanisms of N-acylethanolamines (2020)
  • Author(s): 坪井 一人、宇山 徹、上田 夏生
  • Journal Title: 生化学 Volume: 92 Issue: 5 Pages: 666-679
  • DOI: 10.14952/SEIKAGAKU.2020.920666
  • ISSN: 0037-1017
  • Year and Date: 2020-10-25
  • Peer Reviewed
• [Journal Article] 明らかになり始めた生理活性脂質N-アシルエタノールアミンの生合成機構 (2020)
  • Author(s): 宇山 徹、上田 夏生
  • Journal Title: 化学と生物 Volume: 58 Pages: 599-605
  • Peer Reviewed
• [Journal Article] Intracellular Ca2+-dependent formation of N-acyl-phosphatidylethanolamines by human cytosolic phospholipase A2ε (2019)
  • Author(s): Binte Mustafiz Smriti Sultana、Uyama Toru、Morito Katsuya、Takahashi Naoko、Kawai Katsuhisa、Hussain Zahir、Tsuboi Kazuhito、Araki Nobukazu、Yamamoto Kei、Tanaka Tamotsu、Ueda Natsuo
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Volume: 1864 Issue: 12 Pages: 158515-158515
  • DOI: 10.1016/j.bbalip.2019.158515
  • Peer Reviewed / Open Access
• [Journal Article] アナンダミドとN-アシルエタノールアミンの代謝と疾患制御 (2019)
  • Author(s): 宇山 徹、上田 夏生
  • Journal Title: 医学のあゆみ Volume: 269 Pages: 1015-1022
• [Journal Article] The role of intracellular anionic phospholipids in the production of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A2epsilon (2018)
  • Author(s): Binte Mustafiz Smriti Sultana、Uyama Toru、Hussain Zahir、Kawai Katsuhisa、Tsuboi Kazuhito、Araki Nobukazu、Ueda Natsuo
  • Journal Title: The Journal of Biochemistry Volume: 165 Issue: 4 Pages: 343-352
  • DOI: 10.1093/jb/mvy104
  • Peer Reviewed
• [Journal Article] Endocannabinoids and related N-acylethanolamines: biological activities and metabolism (2018)
  • Author(s): Tsuboi Kazuhito、Uyama Toru、Okamoto Yasuo、Ueda Natsuo
  • Journal Title: Inflammation and Regeneration Volume: 38 Issue: 1 Pages: 28-28
  • DOI: 10.1186/s41232-018-0086-5
  • Peer Reviewed / Open Access
• [Presentation] Phospholipid species used for the Ca2+-dependent formation of N-acyl-phosphatidylethanolamine by cPLA2ε (2020)
  • Author(s): Toru Uyama, Smriti Sultana Binte Mustafiz, Katsuya Morito, Naoko Takahashi, Katsuhisa Kawai, Zahir Hussain, Kazuhito Tsuboi, Nobukazu Araki, Kei Yamamoto, Tamotsu Tanaka, and Natsuo Ueda
  • Organizer: 30th Annual Symposium of the International Cannabinoid Research Society
  • Int'l Joint Research
• [Presentation] N-アシル-ホスファチジルエタノールアミンの生成におけるcPLA2εの細胞内基質の検討 (2020)
  • Author(s): 宇山 徹，Smriti Sultana Binte Mustafiz，森戸克弥，高橋尚子，川合克久，Zahir Hussain，坪井一人，荒木伸一，山本 圭，田中 保，上田夏生
  • Organizer: 第62回日本脂質生化学会
• [Presentation] バイオファクターであるN-アシルエタノールアミンの生合成に関わるcPLA2εの機能解析 (2020)
  • Author(s): 宇山 徹，Smriti Sultana Binte Mustafiz，森戸克弥，高橋尚子，川合克久，Zahir Hussain，坪井一人，荒木伸一，山本 圭，田中 保，上田夏生
  • Organizer: 日本ビタミン学会第72回大会
• [Presentation] 脳虚血モデルでのN-アシル-ホスファチジルエタノールアミンの蓄積はcPLA2εによって引き起こされる (2020)
  • Author(s): 宇山 徹，Zahir Hussain，森戸克弥，田中 保，太田健一，上野正樹，村上 誠，上田夏生
  • Organizer: 第93回日本生化学会大会
• [Presentation] Anionic phospholipids enhance the production of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A2ε (2019)
  • Author(s): Toru Uyama, Zahir Hussain, Smriti Sultana Binte Mustafiz, Katsuhisa Kawai, Kazuhito Tsuboi, Nobukazu Araki, and Natsuo Ueda
  • Organizer: Gordon Research Conferences: Cannabinoid Function in the CNS
  • Int'l Joint Research
• [Presentation] Anionic phospholipids facilitate the formation of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A2ε (2019)
  • Author(s): Toru Uyama, Zahir Hussain, Smriti Sultana Binte Mustafiz, Katsuhisa Kawai, Kazuhito Tsuboi, Nobukazu Araki, and Natsuo Ueda
  • Organizer: 60th International Conference on the Bioscience of Lipids
  • Int'l Joint Research
• [Presentation] cPLA2εによるN-アシル-ホスファチジルエタノールアミンの細胞内カルシウム依存的な生成 (2019)
  • Author(s): 宇山 徹，Smriti Sultana Binte Mustafiz，森戸克弥，高橋尚子，川合克久，Zahir Hussain，坪井一人，荒木伸一，山本 圭，田中 保，上田夏生
  • Organizer: 第92回日本生化学会大会
• [Presentation] N-アシルエタノールアミンの生成に関わるcPLA2εの酸性リン脂質による制御 (2019)
  • Author(s): 宇山 徹，Smriti Sultana Binte Mustafiz，Zahir Hussain，川合克久，坪井一人，荒木伸一，上田夏生
  • Organizer: 日本ビタミン学会第71回大会
• [Presentation] Characterization of cytosolic phospholipase A2ε;: phosphatidylserine-stimulated production of N-acyl-phosphatidylethanolamine (2018)
  • Author(s): Zahir Hussain, Toru Uyama, Katsuhisa Kawai, Smriti Sultana Binte Mustafiz, Kazuhito Tsuboi, Nobukazu Araki and Natsuo Ueda
  • Organizer: 28th Annual Symposium of the International Cannabinoid Research Society
  • Int'l Joint Research
• [Presentation] Analyses of Purified Cytosolic Phospholipase A2ε; from Humans and Mice Reveal the Stimulatory Effect of Phosphatidylserine (2018)
  • Author(s): Zahir Hussain, Toru Uyama, Katsuhisa Kawai, Smriti Sultana Binte Mustafiz, Kazuhito Tsuboi, Nobukazu Araki and Natsuo Ueda
  • Organizer: 第59回日本生化学会中国・四国支部例会
• [Presentation] nhancement of Cytosolic Phospholipase A2ε;-catalyzed Formation of N-Acyl-phosphatidylethanolamine by Phosphatidylserine (2018)
  • Author(s): Zahir Hussain, Toru Uyama, Katsuhisa Kawai, Smriti Sultana Binte Mustafiz, Kazuhito Tsuboi, Nobukazu Araki and Natsuo Ueda
  • Organizer: 第60回日本脂質生化学会
• [Presentation] Analyses of Purified Cytosolic Phospholipase A2ε; from Humans and Mice Reveal the Stimulatory Effect of Phosphatidylserine (2018)
  • Author(s): Zahir Hussain, Toru Uyama, Katsuhisa Kawai, Smriti Sultana Binte Mustafiz, Kazuhito Tsuboi, Nobukazu Araki and Natsuo Ueda
  • Organizer: 第91回日本生化学会大会合同大会
• [Remarks] 香川大学医学部生体分子医学講座生化学
  • URL: http://www.med.kagawa-u.ac.jp/%7Ebiochem/index.html
• [Remarks] 香川大学医学部生体分子医学講座生化学
  • URL: http://www.kms.ac.jp/~biochem/index.html
• [Remarks] 香川大学医学部 生体分子医学講座 生化学
  • URL: http://www.kms.ac.jp/~biochem/index.html