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Analysis of JunB-related cell-cell signaling for neurovascular parallel alignment

Research Project

Project/Area Number 18K06924
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 48040:Medical biochemistry-related
Research InstitutionKanazawa Medical University

Principal Investigator

YOSHITOMI Yasuo  金沢医科大学, 医学部, 講師 (80399039)

Co-Investigator(Kenkyū-buntansha) 高辻 英仁 (齋藤)  金沢医科大学, 医学部, 助教 (40768959)
米倉 秀人  金沢医科大学, 医学部, 教授 (80240373)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords血管新生 / 脈管形成 / JunB / 血管形成 / 神経-血管相互作用 / 神経ー血管相互作用 / 血管リモデリング
Outline of Final Research Achievements

In the present study, to clarify the role of JunB in the process of nerve-blood vessel parallelism, we aimed to identify molecules involved in intercellular signaling by direct nuro-vascular cell attachment and interaction using JunB activation as an indicator to elucidate the mechanism of nerve-vessel parallelism. We generated human vascular endothelial cells with a JunB promoter-reporter and used a gene activation library based on CRISPR-dCas9 for genome-wide screening the molecules that increase the activity of the JunB reporter in response to nerve-vessel contact signals. The results shows that several transcription factors and intracellular signaling molecules were identified in the JunB-induced angiogenesis pathways.

Academic Significance and Societal Importance of the Research Achievements

これまでの研究により神経ー血管並走過程でJunBが誘導されることが神経と血管の並走に必須であることが明らかとなっていたが、JunBがどのような役割を担っているのかは不明であった。神経との接触によって血管内皮細胞にJunBが誘導されるとでTip細胞に転換され血管のリモデリングが誘導される。この過程に関わるシグナル伝達分子の解明は神経との並走を介した血管ワイヤリング機構の理解に繋がり、組織再生での人工血管網の構築方法の開発につながるものと期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (3 results)

  • [Journal Article] Emerging Role of AP-1 Transcription Factor JunB in Angiogenesis and Vascular Development2021

    • Author(s)
      Yoshitomi Yasuo、Ikeda Takayuki、Saito-Takatsuji Hidehito、Yonekura Hideto
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 6 Pages: 2804-2804

    • DOI

      10.3390/ijms22062804

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of Arginine Methylation in Alternative Polyadenylation of VEGFR-1 (Flt-1) pre-mRNA2020

    • Author(s)
      Ikeda Takayuki、Saito-Takatsuji Hidehito、Yoshitomi Yasuo、Yonekura Hideto
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 18 Pages: 6460-6460

    • DOI

      10.3390/ijms21186460

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultraviolet B-induced Otx2 expression in lens epithelial cells promotes epithelial?mesenchymal transition2019

    • Author(s)
      Yoshitomi Yasuo、Osada Hiromi、Satake Haruka、Kojima Masami、Saito-Takatsuji Hidehito、Ikeda Takayuki、Yoshitake Yoshino、Ishigaki Yasuhito、Kubo Eri、Sasaki Hiroshi、Yonekura Hideto
    • Journal Title

      Biology Open

      Volume: 8

    • DOI

      10.1242/bio.035691

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] UB-B照射により水晶体上皮細胞に誘導されるOtx2は水晶体繊維の遺伝子発現を抑制し上皮間葉転換を誘導する2019

    • Author(s)
      吉冨泰央、長田ひろみ、池田崇之、高辻英仁、佐々木洋、米倉秀人
    • Organizer
      第42回分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] UV照射により水晶体上皮細胞で誘導されるOtx2の機能解析2018

    • Author(s)
      吉冨 泰央、長田ひろみ、佐竹 悠、高辻 英仁、池田 崇之、石山 尚史、石垣靖人、久保 江理、佐々木 洋、米倉 秀人
    • Organizer
      第57回白内障学会総会・第44回水晶体研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] UVB照射により水晶体上皮細胞に誘導されるOtx2は上皮間葉転換(EMT)を誘導して水晶体上皮細胞の水晶体繊維への分化を抑制する2018

    • Author(s)
      吉冨 泰央、佐竹 悠、高辻 英仁、池田 崇之、石山 尚史、米倉 秀人
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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