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Elucidation of membrane repair mechanism

Research Project

Project/Area Number 18K06965
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49010:Pathological biochemistry-related
Research InstitutionSapporo Medical University

Principal Investigator

Horio Yoshiyuki  札幌医科大学, 医学部, 教授 (30181530)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsSIRT1 / 細胞膜 / 筋ジストロフィー / 膜修復 / ノックアウトマウス / 骨格筋 / 細胞膜修復 / 運動 / クレアチンキナーゼ / 筋力 / 修復 / 筋芽細胞 / 筋細胞 / muscular dystrophy / membrane repair / mdx mice / imaging
Outline of Final Research Achievements

To elucidate functions of protein deacetylase SIRT1 in skeletal muscles, we generated skeletal muscle-specific SIRT1 knockout (SIRT1-MKO) mice. Skeletal muscle of SIRT1-MKO mice was fragile and showed a phenotype of mild muscular dystrophy, indicating that SIRT1 is indispensable for muscle. Using C2C12 myoblast cells and differentiated C2C12 myofibers, we found that SIRT1 was necessary for cellular membrane resealing after damage. We have shown that a SIRT1 activator, resveratrol, ameliorates pathological and physiological conditions of muscular dystrophies of mice and humans. Our finding of SIRT1 function in membrane resealing would be involved in the effectiveness of resveratrol on dystrophies. Our finding also suggests that an activation of resealing of damaged cellular membrane is a new strategy for the treatment of muscular dystrophies.

Academic Significance and Societal Importance of the Research Achievements

本研究はSIRT1が細胞膜の修復に必要であることを世界で初めて示した。我々はSIRT1活性化薬のレスベラトロールの投与がマウス筋ジストロフィーのみならず人の筋ジストロフィーにも有効であることを示してきたが、そのメカニズムに膜修復の促進が関与することが本研究により示された。これまでエクソンスキッピングによる筋の機能保持が新しい筋ジストロフィー治療法となってはいるが効果はきわめて限定的でさらにきわめて高価な治療費用が必要である。本研究では発想を根本的に変えた膜修復促進という従来とは全く異なる治療戦略が様々なタイプの筋ジストロフィーの治療に応用できる可能性を強く示している。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2020 2019 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) Remarks (2 results)

  • [Journal Article] Resveratrol improves motor function in patients with muscular dystrophies: an open-label, single-arm, phase IIa study2020

    • Author(s)
      Kawamura Kentaro、Fukumura Shinobu、Nikaido Koki、Tachi Nobutada、Kozuka Naoki、Seino Tsugumi、Hatakeyama Kingya、Mori Mitsuru、Ito Yoichi M.、Takami Akiyoshi、Hinotsu Shiro、Kuno Atsushi、Kawasaki Yukihiko、Horio Yoshiyuki、Tsutsumi Hiroyuki
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-020-77197-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SIRT1 deficiency interferes with membrane resealing after cell membrane injury2019

    • Author(s)
      Fujiwara Daisuke、Iwahara Naotoshi、Sebori Rio、Hosoda Ryusuke、Shimohama Shun、Kuno Atsushi、Horio Yoshiyuki
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 6 Pages: e0218329-e0218329

    • DOI

      10.1371/journal.pone.0218329

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] SIRT1 activators ameliorate the ability of cell membrane repair.2020

    • Author(s)
      畔上空也
    • Organizer
      日本薬理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] レスベラトロールの生体作用とその標的としてのタンパク質脱アセチル化酵素SIRT12019

    • Author(s)
      堀尾嘉幸
    • Organizer
      日本生化学会
    • Related Report
      2019 Research-status Report
  • [Remarks] 札幌医科大学薬理学講座ホームページ

    • URL

      https://web.sapmed.ac.jp/pharmacology/index.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 札幌医科大学医学部薬理学講座

    • URL

      https://web.sapmed.ac.jp/pharmacology/index.html

    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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