A search for novel RNA markers for uterine adenocarcinomas

• Project/Area Number: 18K06994
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Nagoya City University
• Principal Investigator: Yamashita Yoriko 名古屋市立大学, 医薬学総合研究院(医学), 研究員 (90303643)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: マイクロRNA / 子宮体癌 / 子宮頚部腺癌 / tissue microarray / 子宮内膜癌 / マイクロアレイ / RNA-seq / in situ hybridization / In situ hybridization / uterine adenocarcinoma / non-coding RNA

Outline of Final Research Achievements

Our idea is to screen for endometrial cancer, which has been increasing in recent years, and cervical adenocarcinoma, which is often found in the advanced stage, using microRNA. A highly sensitive method for screening a large number of cancers for microRNA in blood has already been established, and it is considered that clinical application will be made. In this study, we attempted to use samples of abnormal bleeding from the uterus to screen for these cancers non-invasively. It is expected that clinical application will be made in the future.

Academic Significance and Societal Importance of the Research Achievements

近年増加している子宮体癌と進行期で発見されることが多い子宮頚部腺癌についてマイクロRNAを用いてスクリーニングを行うという画期的なアイデアである。血液中のマイクロRNAについて高感度で多数の癌についてのスクリーニングを行う方法はすでに確立されており、臨床応用がなされるであろうと考えられる。本研究では子宮の不正出血検体を用いて非侵襲的にこれらの癌のスクリーニングを行うという方法である。今後、臨床応用がなされることが期待される。

Research Products

• [Journal Article] Long-term survivor of pulmonary combined large cell neuroendocrine carcinoma treated with nivolumab (2020)
  • Author(s): Oda R, Okuda K, Yamashita Y, Sakane T, Tatematsu T, Yokota K, Endo K, Nakanishi R.
  • Journal Title: Thorac Cancer Volume: 11 Issue: 7 Pages: 2036-2039
  • DOI: 10.1111/1759-7714.13471
  • Peer Reviewed / Open Access
• [Journal Article] Value of TTF-1 expression in non-squamous non-small-cell lung cancer for assessing docetaxel monotherapy after chemotherapy failure. (2020)
  • Author(s): Takeuchi A, Oguri T, Yamashita Y, Sone K, Fukuda S, Takakuwa O, Uemura T, Maeno K, Inoue Y, Yamamoto S, Nishiyama H, Fukumitsu K, Kanemitsu Y, Tajiri T, Ohkubo H, Takemura M, Ito Y, Niimi A
  • Journal Title: Mol Clin Oncol. Volume: 13 Pages: 1-7
  • DOI: 10.3892/mco.2020.2080
  • Peer Reviewed / Open Access
• [Journal Article] The standard form of CD44 as a marker for invasion of encapsulated papillary carcinoma of the breast (2020)
  • Author(s): Kato H, Naiki-Ito A, Yamada T, Suzuki S, Yamashita Y, Inaguma S, Kondo N, Wanifuchi-Endo Y, Toyama T, Takahashi S.
  • Journal Title: Pathology International Volume: 70 Issue: 11 Pages: 835-843
  • DOI: 10.1111/pin.13001
  • Peer Reviewed
• [Journal Article] Overexpression of miR-199/214 is a distinctive feature of iron-induced and asbestos-induced sarcomatoid mesothelioma in rats. (2020)
  • Author(s): Okazaki Y, Chew SH, Nagai H, Yamashita Y, Ohara H, Jiang L, Akatsuka S, Takahashi T, Toyokuni S.
  • Journal Title: Cancer Sci. Volume: 111 Issue: 6 Pages: 2016-2027
  • DOI: 10.1111/cas.14405
  • Peer Reviewed / Open Access
• [Journal Article] Recruitment of miR-8080 by luteolin inhibits androgen receptor splice variant 7 expression in castration-resistant prostate cancer. (2019)
  • Author(s): Naiki-Ito A, Naiki T, Kato H, Iida K, Etani T, Nagayasu Y, Suzuki S, Yamashita Y, Inaguma S, Onishi M, Tanaka Y, Yasui T, Takahashi S.
  • Journal Title: Carcinogenesis Volume: 193 Issue: 8 Pages: 00-00
  • DOI: 10.1093/carcin/bgz193
  • Peer Reviewed / Open Access
• [Journal Article] Expression of P-REX2a is associated with poor prognosis in endometrial malignancies (2018)
  • Author(s): Takeshita Sho、Yamashita Yoriko、Shiomi Kosuke、Suzuki Nako、Yoshida Jun、Naiki-Ito Aya、Suzuki Shugo、Akatsuka Shinya、Toyokuni Shinya、Takahashi Takashi、Mase Shoko、Arakawa Atsushi、Sugiura-Ogasawara Mayumi、Takahashi Satoru
  • Journal Title: Oncotarget Volume: 9 Issue: 37 Pages: 5489-5495
  • DOI: 10.18632/oncotarget.25349
  • Peer Reviewed / Open Access