• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genetic dissection of merozoite invasion by taeget gene analysis of merozoite-specific transcription factors in malaria parasites

Research Project

Project/Area Number 18K07084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49040:Parasitology-related
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Shinzawa Naoaki  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (10583015)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsマラリア / ゲノム編集 / 次世代シーケンサー / ChIP-seq / メロゾイト / 侵入 / NGS / 転写因子 / 次世代シークエンサー
Outline of Final Research Achievements

Erythrocyte invasion by merozoites of malaria parasites is a complex phenomenon consisting of steps such as escape from infected erythrocytes, adhesion to new erythrocytes, apical reorientation, invasion with parasitic spores, and post-invasion stage change. In this study, we attempted to identify novel genes involved in merozoite invasion and elucidate their gene expression mechanisms by analyzing the target genes of AP2M, the master transcription factor of merozoites, using the ChIP-seq method. ChIP-seq analysis of human Plasmodium falciparum AP2M identified 503 target genes and its binding motif. We revealed that AP2M bind to the binding motifs in the upstream sequences of the target genes to activate their transcription.

Academic Significance and Societal Importance of the Research Achievements

マラリア原虫のメロゾイトによる赤血球への侵入は、感染赤血球からの脱出、新しい赤血球への接着、先端部の方向転換、寄生胞を伴う侵入、侵入後のステージ転換などのステップからなる複雑な現象である。本研究で同定した新規侵入関連因子の機能を明らかにしていくことで、赤血球侵入機構の全貌解明につながることが期待される。赤血球侵入期は、マラリア原虫が直接抗体の攻撃にさらされるため、ワクチンの最重要な標的ステージであるといえる。本研究成果により提示される新しい侵入関連遺伝子はワクチン標的分子の候補としてワクチンの開発者に極めて重要な情報となるだろう。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (1 results)

  • [Int'l Joint Research] コロンビア大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] パスツール研究所(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] パスツール研究所(フランス)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase 2 From the Endoplasmic Reticulum in Plasmodium falciparum2021

    • Author(s)
      Taku Izumi、Hirai Tomohiro、Makiuchi Takashi、Shinzawa Naoaki、Iwanaga Shiroh、Annoura Takeshi、Nagamune Kisaburo、Nozaki Tomoyoshi、Saito-Nakano Yumiko
    • Journal Title

      Frontiers in Cellular and Infection Microbiology

      Volume: 10 Pages: 610200-610200

    • DOI

      10.3389/fcimb.2020.610200

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template2020

    • Author(s)
      Shinzawa Naoaki、Nishi Tsubasa、Hiyoshi Fumiya、Motooka Daisuke、Yuda Masao、Iwanaga Shiroh
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 1-1

    • DOI

      10.1038/s42003-020-01138-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Bartonella autotransporter BafA activates the host VEGF pathway to drive angiogenesis2020

    • Author(s)
      Tsukamoto Kentaro、Shinzawa Naoaki、Kawai Akito、Suzuki Masahiro、Kidoya Hiroyasu、Takakura Nobuyuki、Yamaguchi Hisateru、Kameyama Toshiki、Inagaki Hidehito、Kurahashi Hiroki、Horiguchi Yasuhiko、Doi Yohei
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 3571-3571

    • DOI

      10.1038/s41467-020-17391-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bordetella Dermonecrotic Toxin Is a Neurotropic Virulence Factor That Uses CaV3.1 as the Cell Surface Receptor.2020

    • Author(s)
      Teruya S, Hiramatsu Y, Nakamura K, Fukui-Miyazaki A, Tsukamoto K, Shinoda N, Motooka D, Nakamura S, Ishigaki K, Shinzawa N, Nishida T, Sugihara F, Maeda Y, Horiguchi Y.
    • Journal Title

      mBio

      Volume: 11 Issue: 2 Pages: 03146-19

    • DOI

      10.1128/mbio.03146-19

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CRISPR/Cas9 system in Plasmodium falciparum using the centromere plasmid2018

    • Author(s)
      Payungwoung Tongchai、Shinzawa Naoaki、Hino Akina、Nishi Tubasa、Murata Yuho、Yuda Masao、Iwanaga Shiroh
    • Journal Title

      Parasitology International

      Volume: 67 Issue: 5 Pages: 605-608

    • DOI

      10.1016/j.parint.2018.06.002

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Eukaryotic Host Factor 14-3-3 Inactivates Adenylate Cyclase Toxins of Bordetella bronchiseptica and B.?parapertussis, but Not B.?pertussis2018

    • Author(s)
      Fukui-Miyazaki Aya、Toshima Hirono、Hiramatsu Yukihiro、Okada Keisuke、Nakamura Keiji、Ishigaki Keisuke、Shinzawa Naoaki、Abe Hiroyuki、Horiguchi Yasuhiko
    • Journal Title

      mBio

      Volume: 9 Issue: 4 Pages: 1-15

    • DOI

      10.1128/mbio.00628-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Cas9ヌクレアーゼ発現熱帯熱マラリア原虫を用いた新規ゲノム編集法の確立2019

    • Author(s)
      西翔、新澤直明、平山泰士、油田正夫、岩永史朗
    • Organizer
      第88回日本寄生虫学会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi