Visualization of niche where maintains memory B cells in merozoites infection

• Project/Area Number: 18K07091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49040:Parasitology-related
• Research Institution: University of the Ryukyus
• Principal Investigator: KISHIMOTO HIDEHIRO 琉球大学, 医学(系)研究科(研究院), 教授 (80251213)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マラリア / 記憶B細胞 / マラリア感染 / 免疫応答 / B細胞 / 赤血球型マラリア / 抗体 / GFPメロゾイド / MSP-1 / 免疫記憶 / ワクチン

Outline of Final Research Achievements

The purpose of this study is to visualize the field of memory B cell survival during mouse malaria infection and to identify a subset of memory B cells.
In this study, we used mouse malaria that expressing GFP and OVA established in the Department of Biodefense, Gunma University, and to visualize in the mice. OVA-GFP-malaria was infected into mice in which T cells of OVA-specific TCR-Tg mice were transferred, and the immune responses of IFNγ-expressing cells and OT-I and OT-II T cells were analyzed in the various time. As a result, almost no immune response or antibody to OVA was detected. The immunological memory response (secondary response) also resulted in a very weak immune response to the OVA.

Academic Significance and Societal Importance of the Research Achievements

寄生虫であるマラリアは、免疫応答から逃避する様々な機構を持っている。本研究は、ワクチン効果の基礎となる免疫記憶成立の場の同定とそれに関わる重要な記憶B細胞サブセットの同定を当初の目的としていた。マラリアに対する免疫応答を可視化するために用いたOVA-GFP- マラリアを使用し、OVAをマラリアの新たな仮想抗原としたが逆に、マラリアの免疫回避の新たな機構となることが判明した。

Research Products

• [Journal Article] Adipose tissue-derived mesenchymal stem cells ameliorate bone marrow aplasia related with graft-versus-host disease in experimental murine models. (2019)
  • Author(s): Nishi Y, Murakami A, Murayama Y, Tsukahara N, Okamoto S, Nakachi S, Morichika K, Tamaki K, Noguchi H, Matsushita M, Karube K, Fukushima T, Morishima S, Kishimoto H, Masuzaki H
  • Journal Title: Transpl Immunol. Volume: 55 Pages: 1-9
  • Peer Reviewed / Open Access
• [Journal Article] 沖縄近海で獲れる魚介類のアニサキス科幼虫について. (2019)
  • Author(s): 當眞弘，岸本英博
  • Journal Title: 臨床寄生虫誌 Volume: 30 Pages: 77-79
• [Presentation] Bacteriophage-delived peptide vaccine capable of inducing immediate and strong IgG antibody produceion (2019)
  • Author(s): Murakami A, Hashiguchi S, Yonamine S, Suenari Y, Miyahara R, Hashimoto M, Kishimoto H
  • Organizer: nternational Immunological Memory and Vaccine Forum
  • Int'l Joint Research
• [Presentation] Rapid modification of specificity and affinity of anti-influenza VHH antibody using two in vitro evolution methods (2019)
  • Author(s): ishimoto H, Yoshida M, Tsukahara N, Matsubara T, Nakayama H, Murakami A
  • Organizer: Immunology 2019
  • Int'l Joint Research
• [Presentation] 沖縄近海で獲れる魚介類のアニサキス幼虫について－予報－ (2019)
  • Author(s): 當眞弘，岸本英博
  • Organizer: 第30回日本臨床寄生虫学会大会
• [Presentation] ヒトヒフバエDermatobia hominis による皮膚ハエ幼虫症の2例 (2019)
  • Author(s): 當眞弘，半仁田優子，屋我栄，岸本英博
  • Organizer: 第60回日本熱帯医学会大会
• [Presentation] Rapid VHH antibody isolation and in vitro specificity and affinity modifications against diverse influenza hemagglutinins (2019)
  • Author(s): Murakami A, Motohashi M, Tsukahara N, Yonamine S, Koshimoto H.
  • Organizer: The 48th Annual Meeting of the Japanese Society for Immunology