| Project/Area Number |
18K07091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49040:Parasitology-related
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | マラリア / 記憶B細胞 / マラリア感染 / 免疫応答 / B細胞 / 赤血球型マラリア / 抗体 / GFPメロゾイド / MSP-1 / 免疫記憶 / ワクチン |
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| Outline of Final Research Achievements |
The purpose of this study is to visualize the field of memory B cell survival during mouse malaria infection and to identify a subset of memory B cells.
In this study, we used mouse malaria that expressing GFP and OVA established in the Department of Biodefense, Gunma University, and to visualize in the mice. OVA-GFP-malaria was infected into mice in which T cells of OVA-specific TCR-Tg mice were transferred, and the immune responses of IFNγ-expressing cells and OT-I and OT-II T cells were analyzed in the various time. As a result, almost no immune response or antibody to OVA was detected. The immunological memory response (secondary response) also resulted in a very weak immune response to the OVA.
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| Academic Significance and Societal Importance of the Research Achievements |
寄生虫であるマラリアは、免疫応答から逃避する様々な機構を持っている。本研究は、ワクチン効果の基礎となる免疫記憶成立の場の同定とそれに関わる重要な記憶B細胞サブセットの同定を当初の目的としていた。マラリアに対する免疫応答を可視化するために用いたOVA-GFP- マラリアを使用し、OVAをマラリアの新たな仮想抗原としたが逆に、マラリアの免疫回避の新たな機構となることが判明した。
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