| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The unexplained heterocomplex comprised of two dynamin-related proteins in Entamoeba histolytica (EhDrpA and EhDrpB) was investigated to elucidate the rationale of its establishment. This heterocomplex participates in the fission of mitosomes that have undergone reductive evolution of mitochondria. Electron microscopy observations suggested that the EhDrpB homocomplex is unlikely to be involved in mitosomal fission as its size is unsuitable for the fission constriction site. Analyses using the stage conversion model Entamoeba invadens suggested the possibility that quantitative changes in EhDrpA and EhDrpB homologs may regulate mitosomal fission during encystation. Moreover, the differences in the expression profiles between these homologs indicate that the resulting homocomplexes may have different functions during encystation. Therefore, it is thought that the heterocomplex has been established to create functional differentiation through quaternary structure diversification.
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