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Analysis of inter-phage interaction and its impacts on the evolution of enterohemorrhagic E. coli

Research Project

Project/Area Number 18K07116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionKyushu University

Principal Investigator

Nakamura Keiji  九州大学, 医学研究院, 助教 (60706216)

Co-Investigator(Kenkyū-buntansha) 澤口 朗  宮崎大学, 医学部, 教授 (30336292)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords腸管出血性大腸菌 / 志賀毒素ファージ / サテライトファージ / ヘルパーファージ / 志賀毒素(Stx)ファージ / 志賀毒素(Stx)ファージ / Stx2ファージ / バクテリオファージ (ファージ) / ヘルパー/サテライトファージ
Outline of Final Research Achievements

Bacteriophages (or phages) play major roles in the evolution of bacterial pathogens. Multiple phages are often integrated in a host chromosome as prophages, providing various possibilities for prophage-prophage interactions in bacterial cells. The present study investigated an inter-phage interaction between "phage in phage" newly identified in the enterohemorrhagic E. coli (EHEC) O145 strains, and revealed (i) that this coexistence of phages could affect phenotypes of the phages, ii) that phages integrating another prophage may be occurred in strains belonging to various serotypes of EHEC and enteropathogenic E. coli (EPEC), and iii) that this system possibly promote evolution as pathogenic bacteria and genetic diversity in host E. coli. These findings obtained here provide novel insights for not only the research communities working on EHEC/EPEC and their phages but also a wide range of researchers in basic and medical microbiology as well as bacterial evolution.

Academic Significance and Societal Importance of the Research Achievements

様々な菌種において、個々のファージのゲノム構造や宿主菌の進化や多様化における役割が明らかとなっている。しかし、細菌の細胞内に複数存在するファージ間の相互作用とそれが宿主菌の進化や多様化に与えるインパクトは従来の研究ではほぼ未解明の領域であった。この点において、本研究成果が今後のファージ研究に与える影響は小さくない。また、腸管出血性大腸菌は感染患者に重篤な症状を引き起こしうる、公衆衛生上重要な病原細菌である。今回の解析により、本病原細菌の病原性に対する理解においても新しい情報を提供したことで、今後の高病原性大腸菌のサーベイランスなどに貢献できると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2021 2020 2018

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Prophages integrating into prophages: a mechanism to accumulate type III secretion effector genes and duplicate Shiga toxin-encoding prophages in Escherichia coli2021

    • Author(s)
      Nakamura K, Ogura Y, Gotoh Y, Hayashi T.
    • Journal Title

      PLoS Pathogens

      Volume: -

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential dynamics and impacts of prophages and plasmids on the pangenome and virulence factor repertoires of Shiga toxin-producing Escherichia coli O145:H282020

    • Author(s)
      Nakamura K., Murase K., Sato MP., Toyoda A., Itoh T., Mainil JG., Pierard D., Yoshino S., Kimata K., Isobe J., Seto K., Etoh Y., Narimatsu H., Saito S., Yatsuyanagi J., Lee K., Iyoda S., Ohnishi M., Ooka T., Gotoh Y., Ogura Y., Hayashi T.
    • Journal Title

      Microbial Genomics

      Volume: 6 Issue: 1

    • DOI

      10.1099/mgen.0.000323

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] プロファージ内プロファージによる大腸菌への3型分泌エフェクターと志賀毒素遺伝子の蓄積メカニズム2021

    • Author(s)
      中村佳司、小椋義俊、後藤恭弘、林哲也
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] プロファージ内プロファージによる大腸菌への3型分泌エフェクターと志賀毒素遺伝子の蓄積メカニズム2021

    • Author(s)
      中村佳司、小椋義俊、後藤恭弘、林哲也
    • Organizer
      第94回日本細菌学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 腸管出血性大腸菌O26:H11におけるStxファージのゲノム多様性とダイナミクス2021

    • Author(s)
      矢野文悟、中村佳司、谷口愛樹、後藤恭弘、小椋義俊、林哲也
    • Organizer
      第94回日本細菌学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 志賀毒素産生性大腸菌O145:H28におけるプロファージとプラスミドのダイナミクス2020

    • Author(s)
      中村佳司、村瀬一典、佐藤光彦、後藤恭宏、小椋義俊、林哲也
    • Organizer
      第14回日本ゲノム微生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Genome diversity of EHEC O145:H28 and genome-wide search of high Stx2 producibility-associated genes2018

    • Author(s)
      Keiji Nakamura, Kazunori Murase, Atsushi Toyoda, Takehiko Itoh, Jacques Georges Mainil, Shuji Yoshino, Keiko Kimata, Junko Isobe, Kazuko Seto, Yoshiki Etoh, Hiroshi Narimatsu, Shioko Saito, Jun Yatsuyanagi, Sunao Iyoda, Makoto Ohnishi, Tadasuke Ooka, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi
    • Organizer
      10th International symposium on Shiga Toxin producing Escherichia coli Infections (VTEC2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] O157堺株Stx2ファージの形態形成に必須な遺伝子の同定2018

    • Author(s)
      金宗鉉、中村佳司、後藤恭宏、小椋義俊、林哲也
    • Organizer
      第22回腸管出血性大腸菌大腸菌感染症研究会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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