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Development of therapeutic and preventive vaccines for SFTS using recombinant viruses

Research Project

Project/Area Number 18K07144
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49060:Virology-related
Research InstitutionToyama Institute of Health (2020)
University of Toyama (2018-2019)

Principal Investigator

Tani Hideki  富山県衛生研究所, ウイルス部, 部長 (20397706)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsSFTSV / 組換えウイルス / 一回感染性粒子 / ISAAC法 / 中和抗体 / カクテル製剤 / シュードタイプVSV / 1回感染性SFTSV擬似粒子 / 組換えVSV / ワクチン抗原 / SFTSV擬似粒子 / ワクチンベクター / SFTS / VSVベクター / ワクチン
Outline of Final Research Achievements

Specific therapeutic drugs and vaccines for SFTS caused by severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) have not yet been established, and research and development of such anti-SFTSV agents seems to be an urgent issue. In this study, we attempted to develop neutralizing antibodies that can protect against infection using recombinant vesicular stomatitis virus (VSV) or single-round infectious particles of SFTSV (SFTSV-SRIPs). As a result, by using the ISAAC method, a large amount of artificial antibody could be produced from spleen cells of immunized mouse. In addition, it was possible to select cocktail antibodies against Gn and Gc, which have high neutralizing activity, using SFTSV pseudotyped virus.

Academic Significance and Societal Importance of the Research Achievements

現在まで、特異的な治療薬がないSFTSに対して本研究において確立できたカクテル抗体は、これまでになく強固に感染を中和することができるもので、今後、抗体製剤として製品化出来ればSFTSの治療薬としても使えるため社会的意義は大きい。また、本研究で用いた組換え水疱性口内炎ウイルスや一回感染性SFTSV擬似粒子などは免疫抗原としての利用が可能であることが明らかとなり、今後、他のウイルス感染症における抗体医薬品の開発にも応用が効くことがわかり学術的意義もあると思われる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (29 results)

All 2021 2020 2019 2018

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 9 results) Presentation (18 results) Book (2 results)

  • [Journal Article] Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein.2021

    • Author(s)
      Kimura M, Egawa K, Ozawa T, Kishi H, Shimojima M, Taniguchi S, Fukushi S, Fujii H, Yamada H, Tan L, Sano K, Katano H, Suzuki T, Morikawa S, Saijo M, Tani H.
    • Journal Title

      Virology

      Volume: 556 Pages: 124-132

    • DOI

      10.1016/j.virol.2020.10.006

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A highly attenuated vaccinia virus strain LC16m8-based vaccine for severe fever with thrombocytopenia syndrome2021

    • Author(s)
      Yoshikawa T.、Taniguchi S.、Kato H.、Iwata-Yoshikawa N.、Tani H.、Kurosu T.、Fujii H.、Omura N.、Shibamura M.、Watanabe S.、Egawa K.、Inagaki T.、Sugimoto S.、Phanthanawiboon S.、Harada S.、Yamada S.、Fukushi S.、Morikawa S.、Nagata N.、Shimojima M.、Saijo M.
    • Journal Title

      PLOS Pathogens

      Volume: 17 Issue: 2 Pages: 1008859-1008859

    • DOI

      10.1371/journal.ppat.1008859

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activation of platelet-derived growth factor receptor β in the severe fever with thrombocytopenia syndrome virus infection2020

    • Author(s)
      Tani Hideki、Kimura Miyuki、Yamada Hiroshi、Fujii Hikaru、Taniguchi Satoshi、Shimojima Masayuki、Fukushi Shuetsu、Morikawa Shigeru、Saijo Masayuki
    • Journal Title

      Antiviral Research

      Volume: 182 Pages: 104926-104926

    • DOI

      10.1016/j.antiviral.2020.104926

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of the amino acid residue important for fusion of Severe Fever with Thrombocytopenia Syndrome Virus glycoprotein.2019

    • Author(s)
      Hideki Tani, Kengo Kawachi, Miyuki Kimura, Satoshi Taniguchi, Masayuki Shimojima, Shuetsu Fukushi, Manabu Igarashi, Shigeru Morikawa, and Masayuki Saijo.
    • Journal Title

      Virology

      Volume: 535 Pages: 102-110

    • DOI

      10.1016/j.virol.2019.06.014

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gold nanoparticle-adjuvanted S protein induces a strong antigen-specific IgG response against severe acute respiratory syndrome-related coronavirus infection, but fails to induce protective antibodies and limit eosinophilic infiltration in lungs.2019

    • Author(s)
      Hanako Sekimukai, Naoko Iwata-Yoshikawa, Shuetsu Fukushi, Hideki Tani, Michiyo Kataoka, Tadaki Suzuki, Hideki Hasegawa, Kenichi Niikura, Katsuhiko Arai, Noriyo Nagata.
    • Journal Title

      Microbiology and Immunology

      Volume: 64 Issue: 1 Pages: 33-51

    • DOI

      10.1111/1348-0421.12754

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 重症熱性血小板減少症候群(SFTS)2019

    • Author(s)
      谷 英樹
    • Journal Title

      臨床と微生物

      Volume: 46 Pages: 689-695

    • Related Report
      2019 Research-status Report
    • Open Access
  • [Journal Article] Seroprevalence of severe fever with thrombocytopenia syndrome (SFTS) virus antibodies in humans and animals in Ehime prefecture, Japan, an endemic region of SFTS2018

    • Author(s)
      Kimura Toshiya、Fukuma Aiko、Shimojima Masayuki、Yamashita Yasutaka、Mizota Fumi、Yamashita Mayumi、Otsuka Yuka、Kan Miki、Fukushi Shuetsu、Tani Hideki、Taniguchi Satoshi、Ogata Momoko、Kurosu Takeshi、Morikawa Shigeru、Saijo Masayuki、Shinomiya Hiroto
    • Journal Title

      Journal of Infection and Chemotherapy

      Volume: 24 Issue: 10 Pages: 802-806

    • DOI

      10.1016/j.jiac.2018.06.007

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Therapeutic effects of favipiravir against severe fever with thrombocytopenia syndrome virus infection in a lethal mouse model: Dose-efficacy studies upon oral administration2018

    • Author(s)
      Tani Hideki、Komeno Takashi、Fukuma Aiko、Fukushi Shuetsu、Taniguchi Satoshi、Shimojima Masayuki、Uda Akihiko、Morikawa Shigeru、Nakajima Nozomi、Furuta Yousuke、Saijo Masayuki
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 10 Pages: e0206416-e0206416

    • DOI

      10.1371/journal.pone.0206416

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] アレナウイルスの基礎と抗ウイルス薬の現状2018

    • Author(s)
      谷 英樹、浦田秀造
    • Journal Title

      ウイルス

      Volume: 68 Pages: 51-62

    • NAID

      130007651010

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Presentation] in vitroおよびin vivoにおけるハートランドウイルス感染に対するリバビリンおよびファビピラビルの効果の検証2019

    • Author(s)
      1藤井ひかる、谷 英樹、江川和孝、谷口 怜、吉河智城、林昌宏、伊藤(高山)睦代、前木孝洋、黒須 剛、福士秀悦、下島昌幸、宇田晶彦、森川 茂、西條政幸
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 一回感染性重症熱性血小板減少症候群ウイルスの構築2019

    • Author(s)
      谷 英樹、譚 龍、山田博司、木村美幸、谷口 怜、下島昌幸、福士秀悦、佐野 芳、鈴木忠樹、森川 茂、西條政幸
    • Organizer
      第2回SFTS研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] I型インターフェロン受容体欠損マウスに対するSFTSウイルスおよびハートランドウイルスの感染感受性の比較2019

    • Author(s)
      藤井ひかる、谷 英樹、谷口 怜、福士秀悦、吉河智城、山田壮一、林昌宏、伊藤(高山)睦代、前木孝洋、黒須 剛、下島昌幸、宇田晶彦、森川 茂、西條政幸
    • Organizer
      第2回SFTS研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 重症肺炎を引き起こすヒトコロナウイルスの新規ワクチン開発2019

    • Author(s)
      関向華子、岩田奈織子、福士秀悦、福間藍子、谷 英樹、長谷川秀樹、永田典代
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Screening of small chemical compounds inhibiting dengue virus (DENV) infection using DENV single-round infectious particles.2019

    • Author(s)
      Miyuki Kimura, Mami Matsuda, Ryosuke Suzuki, Takasuke Fukuhara, Yoshiharu Matsuura, Hideki Tani
    • Organizer
      The 67th annual meeting of the Japanese society for virology
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysis of anti-cytomegalovirus activity of immunosuppressive agent, Everolimus.2019

    • Author(s)
      Long Tan, Yoshihiro Yoshida, Hideki Tani, and Kimiyasu Shiraki
    • Organizer
      The 67th annual meeting of the Japanese society for virology
    • Related Report
      2019 Research-status Report
  • [Presentation] Generation of single-round infectious particles of severe fever with thrombocytopenia syndrome virus.2019

    • Author(s)
      Long Tan, Hiroshi Yamada, Miyuki Kimura, Satoshi Taniguchi, Masayuki Shimojima, Shuetsu Fukushi, Shigeru Morikawa, Masayuki Saijo, Hideki Tani
    • Organizer
      The 67th annual meeting of the Japanese society for virology
    • Related Report
      2019 Research-status Report
  • [Presentation] ミニゲノムを用いた重症熱性血小板減少症候群ウイルスのゲノム複製・転写を抑制する低分子化合物のスクリーニング2019

    • Author(s)
      山田博司, 木村美幸, 譚 龍, 谷口 怜, 下島昌幸, 福原崇介, 松浦善治, 米納 孝,中嶋 希, 古田要介, 西條政幸, 谷 英樹
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] ルジョウイルスの細胞侵入を阻害する低分子化合物の探索2019

    • Author(s)
      松岡里咲, 木村美幸, 福原崇介, 松浦善治, 西條政幸, 谷 英樹
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] ハートランドウイルス感染マウス致死モデルの作製とこれを用いたリバビリンおよびT-705の治療効果の検証2019

    • Author(s)
      藤井ひかる、谷 英樹、谷口 怜、吉河智城、福士秀悦、山田壮一、原田志津子、林昌宏、伊藤(高山)睦代、前木孝洋、黒須 剛、下島昌幸、宇田晶彦、米納 孝、中嶋 希、古田要介、森川 茂、西條政幸
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] ハートランドウイルス感染における感受性動物モデルの検証2018

    • Author(s)
      藤井ひかる、谷 英樹、江川和孝、谷口 怜、吉河智城、林 昌宏、伊藤睦代、前木孝洋、黒須剛、福士秀悦、下島昌幸、宇田晶彦、森川茂、西條政幸
    • Organizer
      第65回日本実験動物学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] SFTSVおよびHRTV感染におけるリバビリンおよびファビピラビルの抗ウイルス効果の比較2018

    • Author(s)
      藤井ひかる、谷 英樹、谷口 怜、吉河智城、林 昌宏、伊藤睦代、前木孝洋、黒須剛、福士秀悦、下島昌幸、宇田晶彦、米納孝、古田要介、森川茂、西條政幸
    • Organizer
      第一回SFTS研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] ハートランドウイルスエンベロープ蛋白質を外套したシュードタイプVSVの性状解析2018

    • Author(s)
      木村美幸、山田博司、譚龍、西條政幸、谷 英樹
    • Organizer
      Toyama Academic GALA 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] Study of the role of untranslated regions of the S segment genome of Lymphocytic Choriomeningitis Virus2018

    • Author(s)
      Satoshi Taniguchi, Tomoki Yoshikawa, Masayuki Shimojima, Shuetsu Fukushi, Takeshi Kurosu, Hideki Tani, Fumihiro Kato, Takahiro Maeki, Shigeru Tajima, Chang-Kweng Lim, Shigeru Morikawa, Masayuki Saijo
    • Organizer
      The 66th annual meeting of the Japanese society for virology
    • Related Report
      2018 Research-status Report
  • [Presentation] Nelson Bay reovirus σC body domain is associated with strain-specific differences in viral replication2018

    • Author(s)
      Takahiro Kawagishi, Yuta Kanai, Yusuke Sakai, Ryotaro Nouda, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi
    • Organizer
      The 66th annual meeting of the Japanese society for virology
    • Related Report
      2018 Research-status Report
  • [Presentation] Establishiment of an animal model of Heartland virus infection and evaluation of the efficacy of ribavirin and T-705 in vitro and in vivo2018

    • Author(s)
      Hikaru Fujii, Hideki Tani, Kazutaka Egawa, Satoshi Taniguchi, Tomoki Yoshikawa, Chang-Kweng Lim, Mutsuyo Takayama-Ito, Takahiro Maeki, Takeshi Kurosu, Shuetsu Fukushi, Masayuki Shimojima, Akihiko Uda, Shigeru Morikawa, Masayuki Saijo
    • Organizer
      The 66th annual meeting of the Japanese society for virology
    • Related Report
      2018 Research-status Report
  • [Presentation] Activation of platelet derived growth factor receptor β in the infection of severe fever with thrombocytopenia syndrome virus2018

    • Author(s)
      Hideki Tani, Miyuki Kimura, Risa Matsuoka, Taiki Manabe, Hiroshi Yamada, Hikaru Fujii, Satoshi Taniguchi, Shigeru Morikawa, and Masayuki Saijo
    • Organizer
      The 66th annual meeting of the Japanese society for virology
    • Related Report
      2018 Research-status Report
  • [Presentation] Characterization of pseudotyped vesicular stomatitis virus bearing the Heartland virus envelope protein2018

    • Author(s)
      Miyuki Kimura, Kazutaka Egawa, Masayuki Shimojima, Hikaru Fujii, Hiroshi Yamada, Tan Long, Shigeru Morikawa, Masayuki Saijo, Hideki Tani
    • Organizer
      The 66th annual meeting of the Japanese society for virology
    • Related Report
      2018 Research-status Report
  • [Book] 15. Antiviral Drugs for the Therapeutics of SFTS. Severe Fever with Thrombocytopenia Syndrome.2019

    • Author(s)
      Hideki Tani, Masayuki Saijo
    • Total Pages
      12
    • Publisher
      Springer
    • ISBN
      9811395616
    • Related Report
      2019 Research-status Report
  • [Book] グローバル時代のウイルス感染症 新興ウイルス感染症における抗ウイルス薬:ファビピラビル2019

    • Author(s)
      谷 英樹、西條 政幸
    • Total Pages
      6
    • Publisher
      日本医事新報社
    • ISBN
      4784921508
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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