The development of immune cell therapy using iPSC-derived T cells targeting refractory cervical cancers
| Project/Area Number |
18K07273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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| Research Institution | Juntendo University |
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Principal Investigator |
ANDO JUN 順天堂大学, 医学部, 教授 (60348943)
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| Co-Investigator(Kenkyū-buntansha) |
寺尾 泰久 順天堂大学, 医学部, 教授 (00348997)
安藤 美樹 順天堂大学, 医学部, 教授 (10424251)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 子宮頸がん / 免疫細胞療法 / iPS技術 / ヒト・パピローマウイルス特異的細胞傷害性Ｔ細胞 / iPS細胞由来T細胞 / 難治性子宮頸がん / ヒトパピローマウイルス特異的CTL |
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| Outline of Final Research Achievements |
We reprogrammed human papilloma virus (HPV)-specific CTLs by Sendai virus vector. The iPSCs efficiently differentiated into HPV-specific rejuvenated CTLs that demonstrated robust cytotoxicity against cervical cancer. The tumor-suppressive effect of rejuvenated CTLs was stronger and more persistent than that of original peripheral blood CTLs. These rejuvenated HPV-specific CTLs provide a sustained tumor-suppressive effect and constitute promising immunotherapy for cervical cancer.
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| Academic Significance and Societal Importance of the Research Achievements |
iPS技術を用いた免疫細胞療法には大きな可能性がある。子宮頸がんの進行期や再発した症例では、根治的手術や放射線治療の適応とならず、根治は望めず予後不良である。そのため新たな治療戦略が求められている。本研究は健常人ドナーの末梢血からT細胞を誘導し、iPS技術を用いて若返りＴ細胞を作製しin vitroとin vivoで抗腫瘍効果を証明した。 iPS細胞からT細胞へ分化誘導したT細胞は、腫瘍を継続的に根絶し、in vivoで若いメモリーT細胞として長期間生存することが出来た。これらの若返ったT細胞は、持続的な腫瘍抑制効果を認め、将来的に子宮頸癌に対する有望な免疫細胞療法となる可能性がある。
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Sustainable tumor suppressive effect of iPSC-derived rejuvenated T cells targeting cervical cancers.2020
Author(s)
Tadahiro Honda, Miki Ando, Jun Ando, Midori Ishii, Yumi Sakiyama, Kazuo Ohara, Tokuko Toyota, Manami Ohtaka, Ayako Masuda, Yasuhisa Terao, Mahito Nakanishi, Hiromitsu Nakauchi, and Norio Komatsu
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Journal Title
Molecular Therapy
Volume: 28
Issue: 11
Pages: 2394-2405
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Long-term eradication of extranodal natural killer/T-cell lymphoma, nasal type, by induced pluripotent stem cell-derived Epstein-Barr virus-specific rejuvenated T cells in vivo2020
Author(s)
Ando M, Ando J, Yamazaki S, Ishii M, Sakiyama Y, Harada S, Honda T, Yamaguchi T, Nojima M, Ohshima K, Nakauchi H, Komatsu N
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Journal Title
Haematologica
Volume: 105
Issue: 3
Pages: 796-807
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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