| Project/Area Number |
18K07295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
奥野 友介 名古屋大学, 医学部附属病院, 病院講師 (00725533)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ALL / FTA / AML / Iraq / RAS / TCF3-PBX1 / NGS / RAS mutation |
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| Outline of Final Research Achievements |
Diagnostic/remission (paired) samples from 66 Iraqi children with acute leukemia including, 55 acute lymphoblastic leukemia (ALL) cases, and 11 acute myeloid leukemia (AML) cases, were evaluated in Japan using next generation sequencing (NGS). Flinders Technology Associates (FTA) cards used to transfer samples from Iraq, were proved to be good enough to yield an adequate dried blood spot-derived DNA suitable for NGS analysis. Iraqi childhood ALL and AML samples were successfully analyzed. B-ALL represented 49 (89.1%) of ALL cases, and they were categorized into subsets according to the molecular driver, such as chromosomal aneuploidy, rearrangements, and point mutations. Several of these subsets were comparable to the results elsewhere in terms of frequency, except for the striking findings of a highly recurrent TCF3-PBX1 fusion gene of (22.4%) in B-ALL, also a highly frequent RAS signaling pathway mutations of about (45%) was disclosed in each of ALL and AML Iraqi childhood cohorts.
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| Academic Significance and Societal Importance of the Research Achievements |
Through collaboration with Iraqi doctors, we could perform the molecular diagnosis of Iraqi childhood acute leukemia.
Our results showed the unprecedented high frequency of TCF3-PBX1 in Iraqi childhood ALL, and confirmed our previous finding of high RAS mutations in Iraqi childhood acute leukemia.
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