Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Outline of Final Research Achievements |
In this research, we aimed to perform T cell receptor (TCR) repertoire analysis of tumor-infiltrating regulatory T cells (Treg) and determine antigens of the clonally expanded Treg cells using the TCR cloning systems that we had developed. To this end, we developed tumor-bearing mouse using B16F10 cells, prepared tumor infiltrating lymphocyte and analyzed the TCR repertoire. As a result, we determined TCRs that reacted to B16F10 cells. In addition, we have developed an antigen-determination system of CD8+ T cells which enabled us to determine antigens of CD8+ T cells. We will apply the system to determine antigens of the TCRs that we have cloned.
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