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A fundamental study for construction of bile duct cancer early diagnosis method by DNA methylation analysis.

Research Project

Project/Area Number 18K07326
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 50020:Tumor diagnostics and therapeutics-related
Research InstitutionKagoshima University

Principal Investigator

Yokoyama Seiya  鹿児島大学, 医歯学域医学系, 助教 (20569941)

Co-Investigator(Kenkyū-buntansha) 谷本 昭英  鹿児島大学, 医歯学域医学系, 教授 (10217151)
東 美智代  鹿児島大学, 医歯学域鹿児島大学病院, 准教授 (60315405)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords胆管癌 / エピジェネティクス / 予測モデル / 機械学習 / 次世代シークエンス / DNAメチル化 / cholangiocarcinoma / DNA methylation / NGS / BSAS / prognosis prediction / DNAメチル化 / 膵臓癌 / 予後予測 / DNA methyaltion / 次世代シークエンサー / 早期発見 / ムチン / リキッドバイオプシ
Outline of Final Research Achievements

Cholangiocarcinoma is one of the most intractable cancers among all cancers, and it is difficult to diagnose at a stage where early detection as well as cure can be expected. Therefore, it is desirable to establish an early diagnosis method for cholangiocarcinoma and a method to determine the malignancy of mucous-producing cholangiocarcinoma and mucinous cystic cholangiocarcinoma, which have been attracting attention recently. In this project, we constructed a Bisulfite Amplicon Sequencing (BSAS) panel for DNA methylation analysis of 14 cancer-related genes including mucin genes related to the malignancy of cholangiocarcinoma. Then we analyzed the methylation of tumor and non-tumor portions of cholangiocarcinoma tissue specimens (125 cases: 250 specimens including non-neoplastic region and neoplastic region) used this panel. In addition, to evaluated the BSAS panel analysis for clinical application, we compared analysis data with clinical information.

Academic Significance and Societal Importance of the Research Achievements

がん細胞株においてエピジェネティクスによるがん遺伝子・がん抑制遺伝子の発現制御が明らかにされてきた。近年においては、様々なヒト組織において発がん過程におけるがん遺伝子・がん抑制遺伝子の発現とエピジェネティクスの関係が報告されている。そこで、胆管組織検体・胆汁液検体において、胆管腫瘍の悪性度に関連するムチン分子ファミリー遺伝子群を含むがん関連遺伝子の発現と、DNAメチル化の関係性を明らかにできれば、希少性の高い難治がんである、胆管癌の早期発見に向けた臨床応用が可能になると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (5 results)

  • [Journal Article] Predicted Prognosis of Pancreatic Cancer Patients by Machine Learning.2020

    • Author(s)
      Seiya Yokoyama, Taiji Hamada, Michiyo Higashi, Kei Matsuo, Kousei Maemura, Hiroshi Kurahara, Michiko Horinouchi, Tsubasa Hiraki, Tomoyuki Sugimoto, Toshiaki Akahane, Suguru Yonezawa, Marko Kornmann, Surinder K Batra, Michael A Hollingsworth, Akihide Tanimoto
    • Journal Title

      Clinical cancer research

      Volume: - Issue: 10 Pages: 2411-2421

    • DOI

      10.1158/1078-0432.ccr-19-1247

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Next-generation sequencing analysis of endometrial screening liquid-based cytology specimens: a comparative study to tissue specimens2020

    • Author(s)
      Akahane Toshiaki、Kitazono Ikumi、Yanazume Shintaro、Kamio Masaki、Togami Shinichi、Sakamoto Ippei、Nohara Sachio、Yokoyama Seiya、Kobayashi Hiroaki、Hiraki Tsubasa、Suzuki Shinsuke、Ueno Shinichi、Tanimoto Akihide
    • Journal Title

      BMC Medical Genomics

      Volume: 13 Issue: 1 Pages: 101-101

    • DOI

      10.1186/s12920-020-00753-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A tailored next‐generation sequencing panel identified distinct subtypes of wildtype IDH and TERT promoter glioblastomas2020

    • Author(s)
      Higa Nayuta、Akahane Toshiaki、Yokoyama Seiya、Yonezawa Hajime、Uchida Hiroyuki、Takajo Tomoko、Kirishima Mari、Hamada Taiji、Matsuo Kei、Fujio Shingo、Hanada Tomoko、Hosoyama Hiroshi、Yonenaga Masanori、Sakamoto Akihisa、Hiraki Tsubasa、Tanimoto Akihide、Yoshimoto Koji
    • Journal Title

      Cancer Science

      Volume: 111 Issue: 10 Pages: 3902-3911

    • DOI

      10.1111/cas.14597

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells2020

    • Author(s)
      Kitazono Ikumi、Hamada Taiji、Yoshimura Takuya、Kirishima Mari、Yokoyama Seiya、Akahane Toshiaki、Tanimoto Akihide
    • Journal Title

      Laboratory Investigation

      Volume: 100 Issue: 12 Pages: 1551-1563

    • DOI

      10.1038/s41374-020-0462-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 胆管癌におけるDNAメチル化解析2019

    • Author(s)
      横山勢也,東美智代,谷本昭英
    • Organizer
      第108回日本病理学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] DNAメチル化解析結果を用いた計算機科学による膵癌予後予測2019

    • Author(s)
      横山勢也,東美智代,谷本昭英
    • Organizer
      第78回日本癌学会学術総会
    • Related Report
      2019 Research-status Report
  • [Presentation] DNAメチル化解析結果を用いた計算機科学による膵癌予後予測2019

    • Author(s)
      横山勢也,東美智代,谷本昭英
    • Organizer
      第4回黒潮カンファレンス
    • Related Report
      2019 Research-status Report
  • [Presentation] MUC1およびMUC4遺伝子のメチル化解析による膵癌摘出術後の予後評価2018

    • Author(s)
      横山勢也,東美智代,谷本昭英
    • Organizer
      日本病理学会
    • Related Report
      2018 Research-status Report
  • [Presentation] PDAC摘出術後におけるムチン遺伝子のメチル化解析を用いた予後予測法の構築2018

    • Author(s)
      横山勢也,東美智代,谷本昭英
    • Organizer
      日本癌学会学
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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