Development of an improved therapy for stroke based on multiple cell types transplantation
| Project/Area Number |
18K07369
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 51030:Pathophysiologic neuroscience-related
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
長井 篤 島根大学, 学術研究院医学・看護学系, 教授 (40273940)
塩田 由利 島根大学, 学術研究院医学・看護学系, 助教 (10581415)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Cerebral infarction / cell-based therapy / M2-Microglia / Neural stem cells / phagocytosis / Stroke / Multiple cell therapy / Neural stem cell / Microglia / Mesenchymal stem cells / Stroke, / multiple cell therapy / neural stem cells / microglia / mesenchymal stem cells / Cell based therapy |
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| Outline of Final Research Achievements |
The purpose of this study is to develop a cell transplantation-based improved therapy for stroke We transplanted M2 microglia (M2-MG6) first, then neural stem cells (NSCs) in a stroke model. Structural, functional, and molecular changes were assessed.
Stroke volume was decreased, and neurological performances were increased in both NSC-alone or NSC+M2-MG6 groups compared to vehicle group, but no difference between NSC-alone and NSC+M2-MG6 groups. Histologically, cell accumulations and tissue damages were higher in NSC-alone group compared NSC+M2-MG6 group. GFAP+ astrocytes were similar, but Iba-1+ microglia numbers were decreased in NSC+M2-MG6 groups compared to NSC-alone group. Angiogenesis and neuron numbers were increased in NSC+M2-MG6 groups compared to NSC-alone. Moreover, phagocytosis process, and transplanted NSC numbers were increased in NSC+M2-MG6 group. Thus, prior transplantation of M2 microglial cleared the damaged tissues and improved NSC survival in the stroke area.
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| Academic Significance and Societal Importance of the Research Achievements |
脳卒中の病理学における脳卒中領域のミクログリア依存性洗浄の重要性と、その状況で NSC を埋め込むより良い方法であります。
脳卒中は、世界中で死亡および障害の主な原因であり、疾患を完治させる方法は未だ見つかっておりません。この研究は、病気のためのより良い細胞ベースの管理システムを策定するために重要です。
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Report
(4 results)
Research Products
(13 results)
