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Development of an improved therapy for stroke based on multiple cell types transplantation

Research Project

Project/Area Number 18K07369
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 51030:Pathophysiologic neuroscience-related
Research InstitutionShimane University

Principal Investigator

Sheike Abdullah MD  島根大学, 学術研究院医学・看護学系, 助教 (30403447)

Co-Investigator(Kenkyū-buntansha) 長井 篤  島根大学, 学術研究院医学・看護学系, 教授 (40273940)
塩田 由利  島根大学, 学術研究院医学・看護学系, 助教 (10581415)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsCerebral infarction / cell-based therapy / M2-Microglia / Neural stem cells / phagocytosis / Stroke / Multiple cell therapy / Neural stem cell / Microglia / Mesenchymal stem cells / Stroke, / multiple cell therapy / neural stem cells / microglia / mesenchymal stem cells / Cell based therapy
Outline of Final Research Achievements

The purpose of this study is to develop a cell transplantation-based improved therapy for stroke We transplanted M2 microglia (M2-MG6) first, then neural stem cells (NSCs) in a stroke model. Structural, functional, and molecular changes were assessed.
Stroke volume was decreased, and neurological performances were increased in both NSC-alone or NSC+M2-MG6 groups compared to vehicle group, but no difference between NSC-alone and NSC+M2-MG6 groups. Histologically, cell accumulations and tissue damages were higher in NSC-alone group compared NSC+M2-MG6 group. GFAP+ astrocytes were similar, but Iba-1+ microglia numbers were decreased in NSC+M2-MG6 groups compared to NSC-alone group. Angiogenesis and neuron numbers were increased in NSC+M2-MG6 groups compared to NSC-alone. Moreover, phagocytosis process, and transplanted NSC numbers were increased in NSC+M2-MG6 group. Thus, prior transplantation of M2 microglial cleared the damaged tissues and improved NSC survival in the stroke area.

Academic Significance and Societal Importance of the Research Achievements

脳卒中の病理学における脳卒中領域のミクログリア依存性洗浄の重要性と、その状況で NSC を埋め込むより良い方法であります。
脳卒中は、世界中で死亡および障害の主な原因であり、疾患を完治させる方法は未だ見つかっておりません。この研究は、病気のためのより良い細胞ベースの管理システムを策定するために重要です。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2021 2020 2019 2018

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (6 results)

  • [Journal Article] 2.Age-Dependent Analysis of the Effects of Pueraria Decoction on Autonomic2021

    • Author(s)
      Garu A,Shiota Y, Shibly AZ, Sheikh AM, Yano S, Araki T, Zhou X, Azad AK, Nagai A
    • Journal Title

      J Clin Trials

      Volume: 11 Pages: 453-453

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Alteration of Neural Stem Cell Functions in Ataxia and Male Sterility Mice: A Possible Role of β-Tubulin Glutamylation in Neurodegeneration2021

    • Author(s)
      Sheikh AM, Yano S, Tabassum S, Omura K, Araki A, Mitaki S, Ito Y, Huang S, Nagai A.
    • Journal Title

      Cells.

      Volume: 14 Issue: 1 Pages: 155-155

    • DOI

      10.3390/cells10010155

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A cationic gallium phthalocyanine inhibits amyloid β peptide fibril formation.2020

    • Author(s)
      Tabassum S, Sheikh A. M., Yano S, Ikeue T, Mitaki S, Michikawa M and Nagai A
    • Journal Title

      Curr Alzheimer Res

      Volume: 17 Issue: 7 Pages: 1-12

    • DOI

      10.2174/1567205017666201008112002

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association between Cystatin C gene polymorphism and the prevalence of white matter lesion in elderly healthy subjects.2020

    • Author(s)
      Maniwa K, Yano S, Sheikh AM, Onoda K, Mitaki S, Isomura M, Mishima S, Yamaguchi S, Nabika T, Nagai A.
    • Journal Title

      Sci Rep.

      Volume: 10 Issue: 1 Pages: 4068-4068

    • DOI

      10.1038/s41598-020-61383-7

    • NAID

      120007002187

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Mesenchymal stem cell line (B10) increases angiogenesis in a rat MCAO model2019

    • Author(s)
      Sheikh Abdullah Md.、Yano Shozo、Mitaki Shingo、Haque Md. Ahsanul、Yamaguchi Shuhei、Nagai Atsushi
    • Journal Title

      Experimental Neurology

      Volume: 311 Pages: 182-193

    • DOI

      10.1016/j.expneurol.2018.10.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Mesenchymal Stem Cell Line Transplantation Improves Neurological Function and Angiogenesis in Intraventricular Amyloid β-Infused Rats2018

    • Author(s)
      Haque Md. Ahsanul、Sheikh Abdullah Md.、Al Mamun Abdullah、Yano Shozo、Hashimoto Michio、Shido Osamu、Nagai Atsushi
    • Journal Title

      Current Alzheimer Research

      Volume: 15 Issue: 14 Pages: 1331-1342

    • DOI

      10.2174/1567205015666180911145159

    • NAID

      120006776101

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantification of CSF cystatin C using liquid chromatography tandem mass spectrometry.2018

    • Author(s)
      Matsuda C, Shiota Y, Sheikh AM, Okazaki R, Yamada K, Yano S, Minohata T, Matsumoto KI, Yamaguchi S, Nagai A
    • Journal Title

      Clinica Chimica Acta

      Volume: 478 Pages: 1-6

    • DOI

      10.1016/j.cca.2017.12.016

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Co-transplantation of M2 microglia and neural stem cell improves the pathology of cerebral ischemia.2020

    • Author(s)
      Sheikh AM
    • Organizer
      第61回日本神経学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] A cationic gallium phthalocyanine inhibits amyloid beta peptide fibril formation.2020

    • Author(s)
      Tabassum S,Sheikh AM,Yano S,Ikeue T,Ito Y,Mitaki S,Michikawa M,Nagai A
    • Organizer
      第61回日本神経学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Carboxylated Zn-phthalocyanine attenuates brain Aβ in AD model mouse.2020

    • Author(s)
      Azad AK,Sheikh AM,Tabassum S,Okazaki R,Yano S,Michikawa M,Shibly AZ,Zhou X,A Garu,Nagai A
    • Organizer
      第39回日本認知症学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Time-course analysis of ether lipids and ROS in Alzheimer's disease model mouse,2019

    • Author(s)
      長井篤・Azad AK・Sheikh AM・長子晴美・堺 弘道・矢野彰三・道川 誠
    • Organizer
      第38回日本認知症学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysisi of phospholipid species in AD model mouse brain by LC-MS/MS2018

    • Author(s)
      Haque MA, Sheikh AM, 長子晴美、境 弘道、Azad AK, 土屋美加子、道川 誠、長井篤
    • Organizer
      第37回日本認知症学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] MSC transplant decreases Aβand increases angiogenesisi in Aβ-infused rats.2018

    • Author(s)
      Sheik AM,Haque MA,Mamun AA,矢野彰三、橋本道男、紫藤 治、長井 篤
    • Organizer
      第37回日本認知症学会学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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