Relationship between the biological function and/or effect of acupuncture of Aig1l gene discovered by acupuncture stimulation
Project/Area Number |
18K07414
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 52010:General internal medicine-related
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Research Institution | Kobe University |
Principal Investigator |
Takaoka Yutaka 神戸大学, 大学院医学研究科, 客員教授 (20332281)
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Co-Investigator(Kenkyū-buntansha) |
大田 美香 神戸大学, 医学部附属病院, 学術研究員 (20274706)
菅野 亜紀 名古屋大学, 医学部附属病院, 病院助教 (20457039)
鈴木 高史 神戸常盤大学, 保健科学部, 教授 (70305530)
奥野 海良人 つくば国際大学, 医療保健学部, 講師(移行) (50623980)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | Aig1l / 膜タンパク質 / 鍼灸 / 漢方 / 東洋医学 / Aig1l遺伝子 |
Outline of Final Research Achievements |
The purpose of this study is to elucidate the relationship between the function of mouse Acupuncture-induced 1-L (Aig1l) and the acupuncture effect discovered in our study of the mechanism of acupuncture effect. This gene was discovered by homology modeling for each substructure. The 3D structure of Aig1l was first determined by homology modeling. The substructures were combined based on hydrophobic interactions to reveal the overall structure. Furthermore, Aig1l gene expression analysis revealed that Aig1l is specifically expressed in neurons, mainly in the brain, and is almost completely absent in glial cells.
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Academic Significance and Societal Importance of the Research Achievements |
本研究の結果、AIG1Lタンパク質は神経細胞特異的に存在する膜タンパク質であることが判明した。このことは、AIG1L遺伝子が鍼刺激3時間後の細胞から我々が発見した事と併せて考えると、鍼治療の刺激が神経細胞に影響を与えて治療効果を挙げている証拠といえる。この結果は、科学的に鍼灸の有用性を示すものである。 また本研究では、部分理論立体構造解析結果を用いて、タンパク質全体の立体構造解析を可能にする方法を確立した。この結果も本研究成果として意義深く、その応用による研究成果にもつながった。
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Development of an Exon Skipping Therapy for X-linked Alport Syndrome With Truncating Variants in COL4A5.2020
Author(s)
Yamamura T, Horinouchi T, Adachi T, Terakawa M, Takaoka Y, Omachi K, Takasato M, Takaishi K, Shoji T, Onishi Y, Kanazawa Y, Koizumi M, Tomono Y, Sugano S, Shono A, Minamikawa S, Nagano C, Sakakibara N, Ishiko S, Aoto Y, Kamura M, Harita Y, Miura K, Kanda S, Morisada N, and 6 others.
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Journal Title
Nat Commun .
Volume: 11(1)
Issue: 1
Pages: 2777-2777
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] In silico and in vitro analyses of the pathological relevance of the R258H mutation of hepatocyte nuclear factor 4alpha identified in MODY1.2019
Author(s)
Sugawara K, Nomura K, Okada Y, Sugano A, Matsumoto M, Takarada T, Takeuchi A, Awano H, Hirota Y, Nishio H, Takaoka Y, Ogawa W.
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Journal Title
Journal of Diabetes Investigation
Volume: 10
Issue: 3
Pages: 680-684
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Co-precipitation molecules of Hemopexin and Transferrin may be key molecules for fibrillogenesis in the TTR V30M Amyloidogenesis.2018
Author(s)
Mika Ohta, Aki Sugano, Naoya Hatano, Hirotaka Sato, Hirofumi Shimada, Hitoshi Niwa, Toshiyuki Sakaeda, Hajime Tei, Yoshiyuki Sakaki, Ken-ichi Yamamura, Yutaka Takaoka
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Journal Title
Transgenic Research
Volume: 27
Issue: 1
Pages: 15-23
DOI
NAID
Related Report
Peer Reviewed / Open Access
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