Pathophysiological study in non-herpetic acute limbic encephalitis: establishment of assay of abs to AMPA-type GluR
| Project/Area Number |
18K07865
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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| Research Institution | National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorders |
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Principal Investigator |
Takahashi Yukitoshi 独立行政法人国立病院機構(静岡・てんかん神経医療センター臨床研究部), その他部局等, その他 (70262764)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | AMPA型GluR / 自己抗体 / 行動異常 / てんかん / 学習障害 / 脳炎 / 胸腺腫 / 非ヘルペス性急性辺縁系脳炎 / 免疫介在性脳炎 / AMPA型GluR抗体 / 痙攣 |
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| Outline of Final Research Achievements |
We constructed HEK293T cells expressing GluA1, GluA2, GluA3,or GluA4 with EGFP-conjugated vectors, and established immune screening system for antibodies to GluA1, GluA2, GluA3,or GluA4.In the preliminary screening for antibodies to AMPA-type GluRs using CSF from 38 patients of neurological diseases, we found antibodies to GluA1 in two patients, antibodies to GluA2 in one patients, antibodies to GluA3 in one patients, and antibodies to GluA4 in one patients.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトAMPA型GluR（GluA1、GluA2、GluA3、GluA4の各サブユニット）とEGFPの融合タンパクを発現できるベクターを導入したHEK293T細胞を作成し、AMPA型GluR抗体検出が可能となった。今後、AMPA型GluR抗体による神経免疫疾患の診断が可能となり、早期免疫治療が可能となり、予後が改善できる。
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B2020
Author(s)
Yukitoshi Takahashi, Shigeko Nishimura, Emiko Takao, Risa Kasai, Kaoru Enokida, Kuniko Ida, Masataka Fukuoka, Takayoshi Koike, Hiroo Omatsu, Tokito Yamaguchi, Shiho Takano, Tomoyuki Yoshida, Hisashi Mori
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Journal Title
Journal of Neuroimmunology
Volume: 349
Pages: 577427-577427
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prospective HLA-A*31:01 screening and the incidence of carbamazepine-induced cutaneous adverse reactions in Japanese.2018
Author(s)
Mushiroda T, Takahashi Y, Onuma T, Yamamoto Y, Kamei T, Hoshida T, Takeuchi K, Otsuka K, Okazaki M, Watanabe M, Kanemoto K, Oshima T, Watanabe A, Minami S, Saito K, Tanii H et al; GENCAT Study Group.
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Journal Title
JAMA Neurology
Volume: 75
Issue: 7
Pages: 842-849
DOI
Related Report
Peer Reviewed
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[Journal Article] Epilepsy in tuberous sclerosis complex: Findings from the TOSCA Study2018
Author(s)
Nabbout Rima、Belousova Elena、Macaya Alfons、Marques Ruben、O'Callaghan Finbar、Qin Jiong、Sander Valentin、Sauter Matthias、Shah Seema、Takahashi Yukitoshi、Touraine Renaud、Youroukos Sotiris、Zonnenberg Bernard、Jansen Anna、Kingswood John C.、the TOSCA Consortium and TOSCA Investigators
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Journal Title
Epilepsia Open
Volume: 4
Issue: 1
Pages: 73-84
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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