• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

he Impact of Perinatal Stress on Neural Stem Cell Differentiation and Its Mechanisms

Research Project

Project/Area Number 18K07891
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 52050:Embryonic medicine and pediatrics-related
Research InstitutionShowa University (2019, 2023)
Kwansei Gakuin University (2018)

Principal Investigator

Adachi Naoki  昭和大学, 医学部, 准教授 (00450601)

Co-Investigator(Kenkyū-buntansha) 鈴木 辰吾  香川大学, 医学部, 准教授 (50451430)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsグルココルチコイド / 神経幹細胞 / 分化 / ストレス / 神経幹細胞の分化
Outline of Final Research Achievements

This study thoroughly analyzed the impact of glucocorticoids on the differentiation of neural stem cells. In vitro experiments demonstrated that glucocorticoids promote differentiation into astrocytes while inhibiting differentiation into oligodendrocytes. Two intracellular signaling pathways involved in this disruption of differentiation balance were identified. Additionally, next-generation sequencing technology was used to identify the gene groups associated with these processes.

Although in vivo experiments did not progress as initially planned, we established a system for tracking the differentiation fate of neural stem cells using CRISPR-Cas9 genome editing technology. This advancement will enable future analysis of how fetal exposure to glucocorticoids affects brain function later in life.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、グルココルチコイドが神経幹細胞の分化運命に与える影響を分子レベルで解明し、周産期のストレスが長期的な脳機能に与える影響を示したことである。この知見は、精神疾患の予防や治療法の開発に寄与するだけでなく、周産期のストレス管理やステロイド使用のガイドライン策定にも大きく貢献する。
また、グルココルチコイドによる神経幹細胞の運命決定攪乱に関わる細胞内シグナルに関する知見は、今後、胎内ストレスが脳の発達に与える悪影響を緩和するための特定のシグナル経路を標的とした新たな治療戦略の開発に繋がる可能性がある。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Corticotropin-releasing hormone-binding protein is up-regulated by brain-derived neurotrophic factor and is secreted in an activity-dependent manner in rat cerebral cortical neurons.2018

    • Author(s)
      Adachi N, Suzuki S, Matsuoka H, Fushimi S, Ono J, Ohta KI, Hirai Y, Miki T, Koshimizu H.
    • Journal Title

      Journal of Neurochemistry

      Volume: 印刷中 Issue: 1 Pages: 99-110

    • DOI

      10.1111/jnc.14310

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Basic fibroblast growth factor increased glucocorticoid receptors in cortical neurons through MAP kinase pathway2018

    • Author(s)
      Numakawa Tadahiro、Odaka Haruki、Adachi Naoki、Chiba Shuichi、Ooshima Yoshiko、Matsuno Hitomi、Nakajima Shingo、Yoshimura Aya、Fumimoto Kazuhiro、Hirai Yohei、Kunugi Hiroshi
    • Journal Title

      Neurochemistry International

      Volume: 118 Pages: 217

    • DOI

      10.1016/j.neuint.2018.06.009

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Actions of Brain-Derived Neurotrophin Factor in the Neurogenesis and Neuronal Function, and Its Involvement in the Pathophysiology of Brain Diseases2018

    • Author(s)
      Numakawa Tadahiro、Odaka Haruki、Adachi Naoki
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 19 Issue: 11 Pages: 3650-3650

    • DOI

      10.3390/ijms19113650

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 妊娠期ストレスが胎仔の神経幹細胞の細胞系譜決定に及ぼす影響の解析2020

    • Author(s)
      安達直樹
    • Organizer
      第125回日本解剖学会総会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Possible Mechanisms of Astrocyte Senescence in a Hepatic Encephalopathy Model2020

    • Author(s)
      Adachi N, Ando M, Sakhri F.Z, Ikemoto H, Ohashi Y., Tsukada M, Takayama Y, Hirai Y, Sunagawa M.
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Evaluation of antioxidant property, cytotoxicity and toxicity of Cydonia Oblonga fruit extract2020

    • Author(s)
      Sakhri FZ.Adachi N, Kabouche Z, Zerizer S, Sunagawa M
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 妊娠期ストレスが子どもの大脳皮質発達に及ぼす影響の解析2019

    • Author(s)
      安達直樹、池本 英志、砂川 正隆
    • Organizer
      第 9 回日本情動学会
    • Related Report
      2019 Research-status Report
  • [Presentation] A regulatory role for glucocorticoids in glial differentiation from rat neural stem/progenitor cells2018

    • Author(s)
      文元 和弘、鈴木 辰吾、太田 健一、三木 崇範、平井 洋平、安達 直樹
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi