| Project/Area Number |
18K07891
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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| Research Institution | Showa University (2019, 2023)
Kwansei Gakuin University (2018) |
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Principal Investigator |
Adachi Naoki 昭和大学, 医学部, 准教授 (00450601)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 辰吾 香川大学, 医学部, 准教授 (50451430)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | グルココルチコイド / 神経幹細胞 / 分化 / ストレス / 神経幹細胞の分化 |
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| Outline of Final Research Achievements |
This study thoroughly analyzed the impact of glucocorticoids on the differentiation of neural stem cells. In vitro experiments demonstrated that glucocorticoids promote differentiation into astrocytes while inhibiting differentiation into oligodendrocytes. Two intracellular signaling pathways involved in this disruption of differentiation balance were identified. Additionally, next-generation sequencing technology was used to identify the gene groups associated with these processes.
Although in vivo experiments did not progress as initially planned, we established a system for tracking the differentiation fate of neural stem cells using CRISPR-Cas9 genome editing technology. This advancement will enable future analysis of how fetal exposure to glucocorticoids affects brain function later in life.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果は、グルココルチコイドが神経幹細胞の分化運命に与える影響を分子レベルで解明し、周産期のストレスが長期的な脳機能に与える影響を示したことである。この知見は、精神疾患の予防や治療法の開発に寄与するだけでなく、周産期のストレス管理やステロイド使用のガイドライン策定にも大きく貢献する。
また、グルココルチコイドによる神経幹細胞の運命決定攪乱に関わる細胞内シグナルに関する知見は、今後、胎内ストレスが脳の発達に与える悪影響を緩和するための特定のシグナル経路を標的とした新たな治療戦略の開発に繋がる可能性がある。
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