The study to detect early-stage pancreatic cancer based on the data from molecular biology about the atrophic acinar cell surrounding carcinoma in situ.

• Project/Area Number: 18K07897
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Jichi Medical University (2020-2021); Tohoku University (2018-2019)
• Principal Investigator: Kanno Atsushi 自治医科大学, 医学部, 講師 (70509190)
• Co-Investigator(Kenkyū-buntansha): 濱田 晋 東北大学, 医学系研究科, 助教 (20451560) ; 三浦 光一 自治医科大学, 医学部, 准教授 (90375238)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膵癌 / 早期診断 / 腺房細胞 / 膵上皮内癌 / 膵腺房細胞癌 / LMD / プロテオミクス解析 / メタボローム解析 / 膵腺房細胞 / K-ras / Keep1

Outline of Final Research Achievements

To detect the mRNA, miRNA, and several cytokines developing the pancreatic ductal carcinoma and making pancreatic acinar cell atrophy, the histological specimens were obtained from pancreatic ductal carcinoma used by laser capture microdissection. The profile of mRNA, miRNA, and several cytokines are checked by protein array used data to acquire from laser capture microdissection.

Academic Significance and Societal Importance of the Research Achievements

膵癌早期診断に、限局的な膵実質の萎縮が着目されているが、現在その分枝生物学的データの蓄積はない。本研究から明らかにされた、miRNAやサイトカインのデータから、ERCPから採取した膵液を用いて膵癌の早期診断へ寄与するのみではなく、膵発癌に着目した治療への発展が期待される。

Research Products

• [Journal Article] Concordance of the histological diagnosis of type 1 autoimmune pancreatitis and its distinction from pancreatic ductal adenocarcinoma with endoscopic ultrasound-guided fine needle biopsy specimens: an interobserver agreement study. (2022)
  • Author(s): Notohara K, Kamisawa T, Kanno A, et al.
  • Journal Title: Virchows Arch. Volume: 480 Issue: 3 Pages: 565-575
  • DOI: 10.1007/s00428-021-03236-w
  • Peer Reviewed
• [Journal Article] Endoscopic ultrasound-guided pancreatic sampling for the histopathological diagnosis of autoimmune pancreatitis. (2022)
  • Author(s): Kanno A, Miwata T, Nagai H, Ikeda E, Ando K, Kawasaki Y, Tada Y, Yokoyama K, Tamada K, Fukushima N, Kawarai Lefor A, Yamamoto H.
  • Journal Title: Dig Endosc. Volume: 34 Issue: 3 Pages: 420-427
  • DOI: 10.1111/den.14076
  • Peer Reviewed
• [Journal Article] What is the background for the histological diagnosis of autoimmune pancreatitis? (2021)
  • Author(s): Kanno A, Lefor AK, Yamamoto H.
  • Journal Title: Dig Endosc. Volume: 337 Issue: 7 Pages: 1073-1074
  • DOI: 10.1111/den.13917
  • Peer Reviewed
• [Journal Article] Adverse events of endoscopic ultrasound‐guided fine‐needle aspiration for histologic diagnosis in Japanese tertiary centers: Multicenter retrospective study (2021)
  • Author(s): Kanno Atsushi、Yasuda Ichiro、Irisawa Atsushi、et al.
  • Journal Title: Digestive Endoscopy Volume: in press Issue: 7 Pages: 1146-1157
  • DOI: 10.1111/den.13912
  • Peer Reviewed / Open Access
• [Journal Article] The Efficacy and Safety of Balloon Enteroscopy-Assisted Endoscopic Retrograde Cholangiography in Pediatric Patients with Surgically Altered Gastrointestinal Anatomy. (2021)
  • Author(s): Yokoyama K, Yano T, Kanno A, Ikeda E, Ando K, Miwata T, Nagai H, Kawasaki Y, Tada Y, Sanada Y, Tamada K, Lefor AK, Yamamoto H.
  • Journal Title: J Clin Med. Volume: 10 Issue: 17 Pages: 3936-3936
  • DOI: 10.3390/jcm10173936
  • Peer Reviewed
• [Journal Article] Pancreatic Ductal Adenocarcinoma: Epidemiology and Risk Factors. (2021)
  • Author(s): Ushio J, Kanno A, Ikeda E, Ando K, Nagai H, Miwata T, Kawasaki Y, Tada Y, Yokoyama K, Numao N, Tamada K, Lefor AK, Yamamoto H.
  • Journal Title: Diagnostics (Basel). Volume: 11 Issue: 3 Pages: 562-562
  • DOI: 10.3390/diagnostics11030562
  • Peer Reviewed
• [Journal Article] An integrated analysis of host- and tumor-derived markers for predicting high-grade dysplasia and associated invasive carcinoma of intraductal papillary mucinous neoplasms of the pancreas. (2020)
  • Author(s): Hata T, Nakagawa K, Hayashi H, Kanno A,et al.
  • Journal Title: Surg Today. Volume: - Issue: 9 Pages: 1039-1048
  • DOI: 10.1007/s00595-020-01982-z
  • Peer Reviewed
• [Journal Article] IgG4-related Sclerosing Cholangitis Mimicking Cholangiocarcinoma Diagnosed by Endoscopic Ultrasound-guided Fine-needle Aspiration. (2020)
  • Author(s): Matsumoto R, Miura S, Kanno A,et al.
  • Journal Title: Intern Med. Volume: 59 Pages: 945-50
  • NAID: 130007825324
  • Peer Reviewed
• [Journal Article] Nationwide epidemiological survey of autoimmune pancreatitis in Japan in 2016. (2020)
  • Author(s): Masamune A, Kikuta K, Hamada S, Collaborators.
  • Journal Title: J Gastroenterol. Volume: 55 Issue: 4 Pages: 462-70
  • DOI: 10.1007/s00535-019-01658-7
  • Peer Reviewed
• [Journal Article] Recurrence patterns after surgical resection of intraductal papillary mucinous neoplasm (IPMN) of the pancreas; a multicenter, retrospective study of 1074 IPMN patients by the Japan Pancreas Society. (2020)
  • Author(s): Hirono S, Shimizu Y, Ohtsuka T, Kin T, Hara K, Kanno A,et al.
  • Journal Title: J Gastroenterol. Volume: 55 Issue: 1 Pages: 86-99
  • DOI: 10.1007/s00535-019-01617-2
  • Peer Reviewed / Open Access
• [Journal Article] Mixed adenoneuroendocrine carcinoma of the ampulla of Vater: a case report and literature review. (2020)
  • Author(s): Yoshimachi S, Ohtsuka H, Aoki T, Mizuma M, Kanno A,et al.
  • Journal Title: Clin J Gastroenterol. Volume: 13 Issue: 1 Pages: 37-45
  • DOI: 10.1007/s12328-019-01009-2
  • Peer Reviewed
• [Journal Article] Prospective study of early chronic pancreatitis diagnosed based on the Japanese diagnostic criteria. (2019)
  • Author(s): Masamune A, Nabeshima T, Kikuta K, Hamada S, Nakano E, Kume K, Kanno A, et al.
  • Journal Title: J Gastroenterol. Volume: 54 Issue: 10 Pages: 928-35
  • DOI: 10.1007/s00535-019-01602-9
  • Peer Reviewed
• [Journal Article] Preoperative biliary drainage of the hepatic lobe to be resected does not affect liver hypertrophy after percutaneous transhepatic portal vein embolization. (2019)
  • Author(s): Miura S, Kanno A, et al.
  • Journal Title: Surg Endosc. Volume: 34 Issue: 2 Pages: 667-74
  • DOI: 10.1007/s00464-019-06813-y
  • Peer Reviewed
• [Journal Article] Surgery for Pancreatic Neuroendocrine Tumor G3 and Carcinoma G3 Should be Considered Separately. (2019)
  • Author(s): Yoshida T, Hijioka S, Hosoda W, Ueno M, Ikeda M, Ito T, Kanno A, et al.
  • Journal Title: Ann Surg Oncol. Volume: 26 Issue: 5 Pages: 1385-93
  • DOI: 10.1245/s10434-019-07252-8
  • Peer Reviewed
• [Journal Article] Advances in Early Detection of Pancreatic Cancer (2019)
  • Author(s): Kanno Atsushi、Masamune Atsushi、Hanada Keiji、Kikuyama Masataka、Kitano Masayuki
  • Journal Title: Diagnostics Volume: 9 Issue: 1 Pages: 18-18
  • DOI: 10.3390/diagnostics9010018
  • Peer Reviewed
• [Journal Article] Clinical practice guidelines for IgG4-related sclerosing cholangitis (2019)
  • Author(s): Kamisawa Terumi、Kanno Atsushi、Shimosegawa Tooru、Kawa Shigeyuki、Chiba Tsutomu、Okazaki Kazuichi、Takikawa Hajime、Kimura Wataru、Unno Michiaki、Yoshida Masahiro
  • Journal Title: Journal of Hepato-Biliary-Pancreatic Sciences Volume: 26 Issue: 1 Pages: 9-42
  • DOI: 10.1002/jhbp.596
  • NAID: 120007183279
  • Peer Reviewed
• [Journal Article] Diagnostic and Prognostic Impact of Neutrophil-to-Lymphocyte Ratio for Intraductal Papillary Mucinous Neoplasms of the Pancreas With High-Grade Dysplasia and Associated Invasive Carcinoma (2019)
  • Author(s): Hata Tatsuo、Mizuma Masamichi、Motoi Fuyuhiko、Ishida Masaharu、Morikawa Takanori、Takadate Tatsuyuki、Nakagawa Kei、Hayashi Hiroki、Kanno Atsushi、Masamune Atsushi、Kamei Takashi、Furukawa Toru、Naitoh Takeshi、Unno Michiaki
  • Journal Title: Pancreas Volume: 48 Issue: 1 Pages: 99-106
  • DOI: 10.1097/mpa.0000000000001202
  • Peer Reviewed
• [Journal Article] Risk Factors for Pancreatic Stone Formation in Type 1 Autoimmune Pancreatitis: A Long-term Japanese Multicenter Analysis of 624 Patients (2018)
  • Author(s): Ito T, Kawa S, Matsumoto A, Kubota K, Kamisawa T, Okazaki K, Hirano K, Hirooka Y, Uchida K, Masuda A, Ohara H, Shimizu K, Arakura N, Masamune A, Kanno A, Sakagami J, Itoi T, Ito T, Ueki T, Nishino T, Inui K, Mizuno N, Yoshida H, Sugiyama M, Iwasaki E, Irisawa A, Shimosegawa T, Chiba T.
  • Journal Title: Pancreas Volume: 48 Issue: 1 Pages: 49-54
  • DOI: 10.1097/mpa.0000000000001210
  • Peer Reviewed
• [Journal Article] New Model for Predicting Malignancy in Patients With Intraductal Papillary Mucinous Neoplasm (2018)
  • Author(s): Shimizu Yasuhiro、Hijioka Susumu、Hirono Seiko、Kin Toshifumi、Ohtsuka Takao、Kanno Atsushi、Koshita Shinsuke、Hanada Keiji、Kitano Masayuki、Inoue Hiroyuki、Itoi Takao、Ueki Toshiharu、Matsuo Keitaro、Yanagisawa Akio、Yamaue Hiroki、Sugiyama Masanori、Okazaki Kazuichi
  • Journal Title: Annals of Surgery Volume: 45 Issue: 1 Pages: 1-1
  • DOI: 10.1097/sla.0000000000003108
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pancreatic Acinar Cell Carcinoma with Multiple Liver Metastases Effectively Treated by S-1 Chemotherapy (2018)
  • Author(s): Yoshida Naoki、Kanno Atsushi、Masamune Atsushi、Nabeshima Tatsuhide、Hongo Seiji、Miura Shin、Takikawa Tetsuya、Hamada Shin、Kikuta Kazuhiro、Kume Kiyoshi、Ueno Masamichi、Shimosegawa Tooru
  • Journal Title: Internal Medicine Volume: 57 Issue: 24 Pages: 3529-3535
  • DOI: 10.2169/internalmedicine.0294-17
  • NAID: 130007531150
  • Peer Reviewed
• [Journal Article] Clinical guidelines for primary sclerosing cholangitis 2017 (2018)
  • Author(s): Isayama Hiroyuki、PSC guideline committee Members: Kanno Atsushi、Kubota
  • Journal Title: Journal of Gastroenterology Volume: 53 Issue: 9 Pages: 1006-1034
  • DOI: 10.1007/s00535-018-1484-9
  • Peer Reviewed
• [Journal Article] Clinical course of type 1 autoimmune pancreatitis patients without steroid treatment: a Japanese multicenter study of 97 patients. (2018)
  • Author(s): Kubota K, Kamisawa T, Hirano K, Hirooka Y, Uchida K, Ikeura T, Shiomi H, Ohara H, Shimizu K, Arakura N, Kanno A, Sakagami J, Itoi T, Ito T, Ueki T, Nishino T, Inui K, Mizuno N, Yoshida H, Sugiyama M, Iwasaki E, Irisawa A, Okazaki K, Kawa S, Shimosegawa T, Takeyama Y, Chiba T.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 25 Issue: 4 Pages: 223-230
  • DOI: 10.1002/jhbp.541
  • Peer Reviewed
• [Journal Article] Multicenter study of early pancreatic cancer in Japan (2018)
  • Author(s): Kanno Atsushi、Masamune Atsushi、Hanada Keiji、Maguchi Hiroyuki、Shimizu Yasuhiro、Ueki Toshiharu、Hasebe Osamu、Ohtsuka Takao、Nakamura Masafumi、Takenaka Mamoru、Kitano Masayuki、Kikuyama Masataka、Gabata Toshifumi、Yoshida Koji、Sasaki Tamito、Serikawa Masahiro、Furukawa Toru、Yanagisawa Akio、Shimosegawa Tooru
  • Journal Title: Pancreatology Volume: 18 Issue: 1 Pages: 61-67
  • DOI: 10.1016/j.pan.2017.11.007
  • Peer Reviewed
• [Presentation] Usefulness of Franseen FNA needles in histopathological diagnosis of autoimmune pancreatitis (2019)
  • Author(s): Atsushi Kanno et al.
  • Organizer: APA-JPS 50th anniversary meeting
  • Int'l Joint Research
• [Presentation] 当科における自己免疫性膵炎145例の臨床経過 (2019)
  • Author(s): 菅野 敦 ほか
  • Organizer: 第50回 日本膵臓学会 総会
• [Presentation] 炎症性主膵管狭窄とstage0-I膵癌の臨床像の比較 (2019)
  • Author(s): 三浦晋 菅野敦 ほか
  • Organizer: 第105回日本消化器病学会総会