| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Recent studies have shown that fibrotic tissue particularly in the lung is acidic because of enhancement of glycolysis due to tissue hypoxia. This study was aimed to determine whether carbonic anhydrase IX, which regulates extracellular and intracellular pH, plays an important role in the survival and activation of fibroblasts in pulmonary fibrosis. We found that treatment of normal human fibroblasts with transforming growth factor β induced extracellular acidification by stimulating glycolysis. We also found that treatment of bleomycin-exposed mice with U-104, a selective inhibitor of carbonic anhydrase IX, improved lung tissue accumulation of collagen and lung tissue stiffness. These results suggest that CA IX is a therapeutic target for pulmonary fibrosis.
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