Development of renal disease type screening method for clinical application

• Project/Area Number: 18K08219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Keio University
• Principal Investigator: HIRAYAMA Akiyoshi 慶應義塾大学, 政策・メディア研究科(藤沢), 特任講師 (00572405)
• Co-Investigator(Kenkyū-buntansha): 丸山 彰一 名古屋大学, 医学系研究科, 教授 (10362253)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ネフローゼ / メタボローム解析 / 尿 / バイオマーカー / メタボロミクス

Outline of Final Research Achievements

In this study, a comprehensive analysis of urinary metabolites from nephrotic disease patients was conducted by capillary electrophoresis-mass spectrometry (CE-MS) in order to discover biomarkers specific to the type of disease.
As a result, it is possible to identify a novel biomarker for discriminating lupus nephritis and to create a model for discriminating a specific disease from 7 types of nephrotic diseases by combining 2 to 5 kinds of metabolites.

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた新規バイオマーカーは、ループス腎炎の早期診断に使用できるのみならず、治療薬開発や治療効果の判定に役立つ可能性がある。尿は非侵襲に採取できるため、メタボローム解析と組み合わせることによって、より広範な疾患のスクリーニングに使える可能性を秘めている。

Research Products

• [Journal Article] Metabolomic Analysis of Small Extracellular Vesicles Derived from Pancreatic Cancer Cells Cultured under Normoxia and Hypoxia (2021)
  • Author(s): Hayasaka Ryosuke、Tabata Sho、Hasebe Masako、Ikeda Satsuki、Ohnuma Sumiko、Mori Masaru、Soga Tomoyoshi、Tomita Masaru、Hirayama Akiyoshi
  • Journal Title: Metabolites Volume: 11 Issue: 4 Pages: 215-215
  • DOI: 10.3390/metabo11040215
  • Peer Reviewed / Open Access
• [Journal Article] The use of a double coaxial electrospray ionization sprayer improves the peak resolutions of anionic metabolites in capillary ion chromatography-mass spectrometry. (2020)
  • Author(s): Hirayama A, Tabata S, Kudo R, Hasebe M, Suzuki K, Tomita M, Soga T.
  • Journal Title: J Chromatogr A Volume: - Pages: 460914-460914
  • DOI: 10.1016/j.chroma.2020.460914
  • Peer Reviewed
• [Journal Article] Comprehensive Dipeptide Profiling and Quantitation by Capillary Electrophoresis and Liquid Chromatography Coupled with Tandem Mass Spectrometry (2020)
  • Author(s): Ozawa Hitoshi、Hirayama Akiyoshi、Ishikawa Takamasa、Kudo Ryuhei、Maruyama Midori、Shoji Futaba、Doke Tomohito、Ishimoto Takuji、Maruyama Shoichi、Soga Tomoyoshi、Tomita Masaru
  • Journal Title: Analytical Chemistry Volume: 92 Issue: 14 Pages: 9799-9806
  • DOI: 10.1021/acs.analchem.0c01258
  • Peer Reviewed
• [Journal Article] Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis (2020)
  • Author(s): Ozawa Hitoshi、Hirayama Akiyoshi、Shoji Futaba、Maruyama Midori、Suzuki Kumi、Yamanaka-Okumura Hisami、Tatano Hiroshi、Morine Yuji、Soga Tomoyoshi、Shimada Mitsuo、Tomita Masaru
  • Journal Title: Metabolites Volume: 10 Issue: 11 Pages: 442-442
  • DOI: 10.3390/metabo10110442
  • Peer Reviewed / Open Access
• [Journal Article] Lactate production is a prioritized feature of adipocyte metabolism (2019)
  • Author(s): Krycer James R.、Quek Lake-Ee、Francis Deanne、Fazakerley Daniel J.、Elkington Sarah D.、Diaz-Vegas Alexis、Cooke Kristen C.、Weiss Fiona C.、Duan Xiaowen、Kurdyukov Sergey、Zhou Ping-Xin、Tambar Uttam K.、Hirayama Akiyoshi、Ikeda Satsuki、Kamei Yushi、Soga Tomoyoshi、Cooney Gregory J.、James David E.
  • Journal Title: Journal of Biological Chemistry Volume: 295 Issue: 1 Pages: 83-98
  • DOI: 10.1074/jbc.ra119.011178
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] メタボロミクスによるネフローゼ症候群の尿中バイオマーカー探索 (2020)
  • Author(s): 平山明由、秋山真一、丸山彰一、曽我朋義
  • Organizer: 第68回 質量分析総合討論会
• [Presentation] メタボロミクスと疾患研究 (2019)
  • Author(s): 平山 明由
  • Organizer: 第36回 和漢医薬学会学術大会
  • Invited
• [Presentation] Metabolomics in biology research (2019)
  • Author(s): Akiyoshi Hirayama
  • Organizer: 第42回 日本分子生物学会年会
• [Presentation] メタボローム解析技術を用いた糖尿病腎症をはじめとする各種腎疾患のバイオマーカー探索 (2018)
  • Author(s): 平山明由
  • Organizer: 第61回 日本糖尿病学会年次学術集会
  • Invited
• [Patent(Industrial Property Rights)] 全身性エリテマトーデスの検出方法 (2019)
  • Inventor(s): 平山明由、曽我朋義、丸山彰一、秋山真一
  • Industrial Property Rights Holder: 平山明由、曽我朋義、丸山彰一、秋山真一
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
  • Overseas
• [Patent(Industrial Property Rights)] 全身性エリテマトーデスの検出方法 (2018)
  • Inventor(s): 平山明由、曽我朋義、丸山彰一、秋山真一
  • Industrial Property Rights Holder: 平山明由、曽我朋義、丸山彰一、秋山真一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-242371
  • Filing Date: 2018