Role of human papilloma virus E6/E7 and CD55 positive cells in skin carcinogenesis
| Project/Area Number |
18K08260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53050:Dermatology-related
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中野 創 弘前大学, 医学研究科, 准教授 (90281922)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 皮膚有棘細胞癌 / ウイルス発癌 / 発癌 / 皮膚悪性腫瘍 / 細胞増殖 / パピローマウイルス / 皮膚発癌 / HPV |
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| Outline of Final Research Achievements |
We performed PCR to detect HPV DNA in primary cutaneous SCCs/Bowen's diseases. We found that HPV-16 was more frequently detected in tumors developed in genital area than in those developed in other site, indicating that HPV is related to the mechanisms of developing genial tumors. Cell growth and migration tests did not show significant difference between E6 overexpressing SCC cultured cells and control cells. CD55 IHC revealed more positive cells in HPV+ SCC tissues than in control tumor samples.
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| Academic Significance and Societal Importance of the Research Achievements |
皮膚有棘細胞癌は皮膚癌の中で二番目に多い腫瘍である。本研究で、特に外陰部の有棘細胞癌においてはヒトパピローマウイルス（HPV）が発生に関与している可能性が示された。HPVの遺伝子を皮膚由来細胞に発現させただけでは腫瘍の性質は変化しなかったため、腫瘍形質獲得には別な機序が関与していることが示唆された。なお、HPVが関与する腫瘍ではCD55陽性細胞が多く検出されることがわかり、将来の研究の基盤となる結果が得られた。
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Report
(4 results)
Research Products
(9 results)
