Role of human papilloma virus E6/E7 and CD55 positive cells in skin carcinogenesis

• Project/Area Number: 18K08260
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Hirosaki University
• Principal Investigator: Rokunohe Daiki 弘前大学, 医学研究科, 助教 (50436036)
• Co-Investigator(Kenkyū-buntansha): 中野 創 弘前大学, 医学研究科, 准教授 (90281922)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 皮膚有棘細胞癌 / ウイルス発癌 / 発癌 / 皮膚悪性腫瘍 / 細胞増殖 / パピローマウイルス / 皮膚発癌 / HPV

Outline of Final Research Achievements

We performed PCR to detect HPV DNA in primary cutaneous SCCs/Bowen's diseases. We found that HPV-16 was more frequently detected in tumors developed in genital area than in those developed in other site, indicating that HPV is related to the mechanisms of developing genial tumors. Cell growth and migration tests did not show significant difference between E6 overexpressing SCC cultured cells and control cells. CD55 IHC revealed more positive cells in HPV+ SCC tissues than in control tumor samples.

Academic Significance and Societal Importance of the Research Achievements

皮膚有棘細胞癌は皮膚癌の中で二番目に多い腫瘍である。本研究で、特に外陰部の有棘細胞癌においてはヒトパピローマウイルス（HPV）が発生に関与している可能性が示された。HPVの遺伝子を皮膚由来細胞に発現させただけでは腫瘍の性質は変化しなかったため、腫瘍形質獲得には別な機序が関与していることが示唆された。なお、HPVが関与する腫瘍ではCD55陽性細胞が多く検出されることがわかり、将来の研究の基盤となる結果が得られた。

Research Products

• [Journal Article] Significance of IL36RN mutation analyses in the management of impetigo herpetiformis: A case report and review of published cases (2021)
  • Author(s): Yoshikawa Miyuki、Rokunohe Daiki、Kimura Atsuko、Takahashi Mika、Korekawa Ayumi、Nakajima Koji、Nakano Hajime、Yokoyama Minako、Tanaka Kanji、Yokoyama Yoshihito、Sawamura Daisuke
  • Journal Title: The Journal of Dermatology Volume: - Issue: 5 Pages: 699-702
  • DOI: 10.1111/1346-8138.15788
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of the mechanism underlying a mild phenotype of hereditary coproporphyria due to a homozygous missense mutation in the transcription initiation codon of the coproporphyrinogen III oxidase gene (2020)
  • Author(s): Fukui Tomohisa、Akasaka Eijiro、Rokunohe Daiki、Matsuzaki Yasushi、Sawamura Daisuke、Kabashima Kenji、Nakano Hajime
  • Journal Title: Journal of Dermatological Science Volume: 100 Issue: 2 Pages: 156-159
  • DOI: 10.1016/j.jdermsci.2020.06.006
  • Peer Reviewed
• [Journal Article] Occupational ionizing radiation-induced skin injury among orthopedic surgeons: A clinical survey (2020)
  • Author(s): Asari Toru、Rokunohe Daiki、Sasaki Eiji、Kaneko Takahide、Kumagai Gentaro、Wada Kanichiro、Tanaka Sunao、Sawamura Daisuke、Ishibashi Yasuyuki
  • Journal Title: Journal of Orthopaedic Science Volume: - Issue: 1 Pages: 266-271
  • DOI: 10.1016/j.jos.2020.11.008
  • Peer Reviewed
• [Journal Article] The first Japanese case of familial porphyria cutanea tarda diagnosed by a UROD mutation (2019)
  • Author(s): Matsui Akinobu、Akasaka Eijiro、Rokunohe Daiki、Matsuzaki Yasushi、Sawamura Daisuke、Nakano Hajime
  • Journal Title: Journal of Dermatological Science Volume: 93 Issue: 1 Pages: 65-67
  • DOI: 10.1016/j.jdermsci.2018.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Nagashima‐type palmoplantar keratoderma and malignant melanoma in Japanese patients (2019)
  • Author(s): Korekawa A, Akasaka E, Rokunohe D, Fukui T, Kaneko T, Sawamura D, Ishikawa M, Yamamoto T, Nakano H.
  • Journal Title: British Journal of Dermatology Volume: 180 Issue: 2 Pages: 415-416
  • DOI: 10.1111/bjd.17251
  • Peer Reviewed / Open Access
• [Presentation] BRAF・MEK阻害薬のリチャレンジが奏功した進行期悪性黒色腫 (2021)
  • Author(s): 六戸大樹，相樂千尋，中野 創，澤村大輔，金子高英
  • Organizer: 第36回日本皮膚悪性腫瘍学会学術大会
• [Presentation] 灰黒色調を呈した下眼瞼表皮嚢腫 (2020)
  • Author(s): 吉川 未雪, 六戸 大樹, 高橋 実か, 是川 あゆ美, 中島 康爾, 中野 創, 澤村 大輔
  • Organizer: 日本皮膚科学会東部支部学術大会
• [Presentation] ニボルマブ/イピリムマブ併用療法後に重症筋無力症を生じた悪性黒色腫 当科での併用療法のまとめとともに (2019)
  • Author(s): 六戸大樹、金子高英、古川和仁、相樂千尋、中野 創、澤村大輔
  • Organizer: 第35回日本皮膚悪性腫瘍学会
• [Presentation] Dabrafenib+Trametinib併用療法によって生じた発熱性好中球減少症 (2019)
  • Author(s): 金子高英、福井智久、相樂千尋、滝吉典子、六戸大樹、会津隆幸、澤村大輔、須賀 康
  • Organizer: 第35回日本皮膚悪性腫瘍学会