| Project/Area Number |
18K08265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53050:Dermatology-related
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| Research Institution | University of Toyama |
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Principal Investigator |
Teruhiko Makino 富山大学, 学術研究部医学系, 准教授 (90359711)
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| Co-Investigator(Kenkyū-buntansha) |
清水 忠道 富山大学, 学術研究部医学系, 教授 (70260396)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | S100 fused-type protein / フィラグリン / アトピー性皮膚炎 / S100 fused-type蛋白質 / 表皮角化細胞 / 皮膚バリア / 角化 / S100 fused-type蛋白質群 |
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| Outline of Final Research Achievements |
In the present study, we comprehensively examined an association of S100 fused-type protein family, including filaggrin (FLG), filaggrin-2 (FLG2), hornerin (HRNR), trichohyalin (TCHH), trichohyalin like 1 (TCHHL1), repetin (RPTN) and cornulin (CRNN), in the barrier formation in the atopic skin. To induce atopic dermatitis (AD) skin model, 3D skin equivalents were stimulated with recombinant IL-4 and IL-13. In this skin model, expression of FLG and FLG2 were significantly decreased and expression of RPTN and TCHH were significantly increased. On the other hand, expression of FLG, CRNN, TCHHL1 were significantly decreased and expression of FLG2, HRNR and TCHH were significantly increased. These results suggest that a regulation of the expression and a function in the barrier formation in the atopic skin may be different among S100 fused-type protein family members.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の特色はFLGとそれに類似するS100 fused-type蛋白質群の全ての分子の機能解析を同時に行ったことにある。これにより各分子の皮膚の組織構築への関与を比較検討することが可能となり、ヒト表皮角化細胞を用いた3次元皮膚モデルの解析により、ヒト皮膚における各分子の機能と疾患との関連を直接解明できる。本研究で皮膚のバリア形成において重要な役割を担い、さらにアトピー性皮膚炎と関連しうる分子が同定されつつある。さらに研究が進展すればアトピー性皮膚炎の病態解明のみならず新規治療法の開発にもつながる可能性があり、臨床医学において大きな意味を持つといえる。
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