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Analysis of master negative regulator Rest as a stem cell regulator by using melanocyte lineage

Research Project

Project/Area Number 18K08294
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 53050:Dermatology-related
Research InstitutionGifu University

Principal Investigator

AOKI Hitomi  岐阜大学, 大学院医学系研究科, 講師 (10550361)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords神経分化抑制因子 / Rest / 幹細胞 / 色素細胞 / 遺伝子組み換えマウス / 神経堤細胞 / 未分化維持 / 未分化性維持 / 神経分化抑制因子Rest / 色素幹細胞 / 幹細胞因子 / 加齢変化 / 成体幹細胞
Outline of Final Research Achievements

The neural differentiation inhibitor Rest has various functions other than regulation of neural differentiation, and is involved in maintenance of homeostasis, stress response, carcinogenesis, etc. Rest is expressed in the stem cells in embryos and affects their differentiation, but the function of Rest in adult tissue stem cells is unclear. We previously reported that Rest contributes to the differentiation of neural crest cells into melanocyte stem cells. However, the function of Rest in adult melanocyte stem cells, which influences hair and skin color throughout life, has not been elucidated.
In this study, we analyzed the role of Rest in melanocyte stem cells using an experimental model of age-related hair-graying model and genetically modified mice that can regulate the expression of Rest, and made efforts aimed to elucidate the function of Rest as a differentiation regulator of melanocyte stem cells.

Academic Significance and Societal Importance of the Research Achievements

白髪化は、色素幹細胞やそのニッシェである毛包幹細胞の何らかの異常により生じるが、その発生頻度の高さや心理的な影響の大きさにも関わらず、有効な改善法や効果的な予防法は確立されていない。また、早期白髪化などでの特定の遺伝子が原因である場合を除き、加齢性の白髪化では、その発生や進行、頻度に個人差や人種差が大きく、遺伝的な要因と環境要因などの影響を受けていると思われる。
転写調節因子RestによるMitfを介した色素幹細胞への作用には、エピジェネティックな要因も関連しており、本研究で示された可能性は、将来的な白髪化の予防や改善に貢献する手がかりとなると思われる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (15 results)

All 2023 2022 2021 2020 2019 2018

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (2 results)

  • [Journal Article] Compensatory gene expression potentially rescues impaired brain development in Kit mutant mice2023

    • Author(s)
      Minei R, Aoki H, Ogura A, Kunisada T
    • Journal Title

      Sci Rep

      Volume: 13;13(1) Issue: 1 Pages: 4166-4166

    • DOI

      10.1038/s41598-023-30032-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 白髪化のメカニズムとその抑制に有効な因子の探索2022

    • Author(s)
      青木仁美
    • Journal Title

      毛髪科学

      Volume: 128 Pages: 17-23

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of hydroxygenkwanin against hair graying induced by X-ray irradiation and repetitive plucking2021

    • Author(s)
      Nobuhiko Taguchi, Ryosuke Kitai, Takuya Ando, Toshihiro Nishimura, Hitomi Aoki, Takahiro Kunisada
    • Journal Title

      JID Innovations

      Volume: 1 Issue: 4 Pages: 1-31

    • DOI

      10.1016/j.xjidi.2022.100121

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induced genetic ablation of Rest leads to the alteration of stimulus‐induced response of the vagal nerve2021

    • Author(s)
      Aoki Hitomi、Abe Chikara、Hara Akira、Miyazaki Tatsuhiko、Morita Hironobu、Kunisada Takahiro
    • Journal Title

      Genes to Cells

      Volume: 26 Issue: 2 Pages: 45-55

    • DOI

      10.1111/gtc.12819

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dietary <i>Eriodictyon angustifolium</i> Tea Supports Prevention of Hair Graying by Reducing DNA Damage in CD34+ Hair Follicular Keratinocyte Stem Cells2020

    • Author(s)
      Taguchi N, Homma T, Aoki H, Kunisada T.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 43 Issue: 10 Pages: 1451-1454

    • DOI

      10.1248/bpb.b20-00455

    • NAID

      130007920879

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2020-10-01
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 白髪のメカニズムと改善を目指した取り組み2020

    • Author(s)
      青木仁美
    • Journal Title

      日皮協ジャーナル

      Volume: 42(83) Pages: 27-40

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Eriodictyon angustifolium extract, but not Eriodictyon californicum extract, reduces human hair greying.2020

    • Author(s)
      Taguchi N, Hata T, Kamiya E, Homma T, Kobayashi A, Aoki H, Kunisada T.
    • Journal Title

      Int J Cosmet Sci.

      Volume: 10 Issue: 4 Pages: 1-10

    • DOI

      10.1111/ics.12620

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reduction in human hair graying by sterubin, an active flavonoid of Eriodictyon angustifolium2018

    • Author(s)
      Taguchi Nobuhiko、Hata Toshihiro、Kamiya Emi、Kobayashi Ai、Aoki Hitomi、Kunisada Takahiro
    • Journal Title

      Journal of Dermatological Science

      Volume: 92 Issue: 3 Pages: 286-289

    • DOI

      10.1016/j.jdermsci.2018.11.002

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Kitl(SCF)の発現誘導による毛包色素幹細胞の分化制御2022

    • Author(s)
      青木仁美
    • Organizer
      第31回日本色素細胞学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 白髪化のメカニズムとその抑制に有効な因子の探索2021

    • Author(s)
      青木仁美
    • Organizer
      第 45 回毛髪技術者協会 西日本学術大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Rest (RE-1 silencing transcription factor) affects mouse hair pigmentation2019

    • Author(s)
      Hhitomi AOKI
    • Organizer
      JAPAN-SINGAPORE INTERNATIONAL SKIN CONFERENCE 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 色素幹細胞の維持機構と白髪の発症メカニズム2019

    • Author(s)
      青木仁美
    • Organizer
      第19回日本抗加齢医学会総会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 白髪のメカニズムと改善を目指した取り組み2019

    • Author(s)
      青木仁美
    • Organizer
      日本産業皮膚衛生協会 夏季セミナー
    • Related Report
      2019 Research-status Report
    • Invited
  • [Book] 毛髪科学の新展開2019

    • Author(s)
      監修:岩渕徳郎
    • Total Pages
      283
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314341
    • Related Report
      2019 Research-status Report
  • [Book] 「最新・化粧品開発のための美容理論、処方/製剤、機能評価の実際」2018

    • Author(s)
      正木 仁・鈴木敏幸・安藤秀哉 編集
    • Total Pages
      420
    • Publisher
      技術教育出版(有)
    • ISBN
      9784907837273
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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