Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Outline of Final Research Achievements |
Resident epidermal T cells of the murine skin express an invariant γδ T-cell receptor (Vγ3 TCR) that recognizes an undetermined self ligand expressed on epidermal keratinocytes, and produce IL-13 upon TCR stimulation. Their fetal thymic precursors do not produce IL-13 and acquire IL-13-producing capacity in the perinatal epidermis. Analyses of cytokine production by resident epidermal T cells in Tcrd-/- and Tcrd-V1-/- mice revealed that only epidermal T cells expressing TCR that can recognize the self ligand on epidermal keratinocytes produce IL-13. We established a TCR stimulation-mediated in vitro differentiation model of neonatal epidermal Vγ3 T cells. By adding inhibitors of downstream signaling pathways of TCR, we identified a candidate signaling pathway specific for the differentiation of IL-13-producing cells.
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