| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We performed array analysis using cultured fibroblasts derived from scleroderma skin, and focused on a cyclic RNA (X) as a circular RNA that has more than 50 reads and can be detected by the taqman system. When the circular RNA(X) was forcibly expressed in normal fibroblasts using a lentivirus, the protein expression of type I collagen and CTGF was suppressed.
In addition, we extracted RNA from serum and tried to confirm whether the circular RNA(X) was actually expressed in serum by real-time PCR using a small number of samples, but there was no amplification and we could not detect the RNA in serum in our experimental system.
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