Genomic and epidenomic analysis of bacteria causing Buruli ulcer
Project/Area Number |
18K08312
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 53050:Dermatology-related
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
Hoshino Yoshihiko 国立感染症研究所, ハンセン病研究センター 感染制御部, 室長 (20569694)
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Co-Investigator(Kenkyū-buntansha) |
深野 華子 国立感染症研究所, ハンセン病研究センター 感染制御部, 研究員 (40807541)
酒井 香奈江 神戸大学, 科学技術イノベーション研究科, 特命助教 (60599447)
吉田 光範 国立感染症研究所, ハンセン病研究センター 感染制御部, 主任研究官 (70772630)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 抗酸菌 / ゲノム解析 / ゲノムメチル化 / 病原性 / プラスミド / 全ゲノム解析 / 抗酸菌外毒素 / 菌側因子 / 新規抗酸菌薬開発 |
Outline of Final Research Achievements |
By 2018, more than 10 strains of Bruli ulcer-causing bacteria have been analyzed for whole genome nucleic acids using next-generation sequencers (NGS). It was found that the isolates had regional differences, and similar species were solidified in each isolated region. Methylation status of 8 strains of bacteria causing Bruli ulcers was analyzed using NGS. It was suggested that the degree of methylation differed in the whole genome region between African and Asian strains. Although we have conducted infection experiments on mice with a unified background of African and Asian strains, average Nucleartide Identity shows that the pathogenicity is different. Considering the possibility that the degree of methylation may be involved in the 2020 fiscal year, the methylation of more strains was analyzed with NGS, and the differences between the African and Asian strain populations were clarified, so this result was scheduled to be presented collectively as a paper.
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Academic Significance and Societal Importance of the Research Achievements |
アフリカ株とアジア株が異なることが示されつつあり、ブルーリ海洋に対する対策にも地域差を考慮する必要があることが示唆されつつあり、国際的疫学研究にもインパクトのある成果となりつつある。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Bursitis, Bacteremia, and Disseminated Infection of <i>Mycobacteroides</i> (<i>Mycobacterium</i>) <i>abscessus</i> subsp. <i>massiliense</i>2021
Author(s)
Oka K, Morioka H, Asakura T, Sato Y, Tetsuka N, Iguchi M, Kanematsu T, Fukano H, Hoshino Y, Kiyoi H,Yagi T.
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Journal Title
Internal Medicine
Volume: 60
Issue: 18
Pages: 3041-3045
DOI
NAID
ISSN
0918-2918, 1349-7235
Year and Date
2021-09-15
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nutritional status positively impacts humoral immunity against its Mycobacterium tuberculosis, disease progression, and vaccine development.2020
Author(s)
Mamiko Niki, Takashi Yoshiyama, Hideaki Nagai, Yuji Miyamoto, Makoto Niki, Ken-Ichi Oinuma, Taishi Tsubouchi, Yukihiro Kaneko, Sohkichi Matsumoto, Yuka Sasaki, Yoshihiko Hoshino
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Journal Title
PloS one
Volume: 15
Issue: 8
Pages: e0237062-e0237062
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sitafloxacin-Containing Regimen for the Treatment of Refractory?Mycobacterium avium?Complex Lung Disease.2019
Author(s)
Asakura T, Suzuki S,Fukano H, Okamori S, Kusumoto T, Uwamino Y, Ogawa T, So M, Uno S, Namkoong H, Yoshida M, Kamata H, Ishii M, Nishimura T, Hoshino Y, Hasegawa N.
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Journal Title
Open forum infectious diseases
Volume: 6
Issue: 4
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Draft Genome Sequence of Mycolicibacterium sp. Strain NCC-Tsukiji, Isolated from Blood Culture of a Patient with Malignant Lymphoma2019
Author(s)
Fukano H, Yoshida M, Shouji M, Hatta S, Maruyama D, Izutsu K, Shiotsuka M, Ogura Y, Hayashi T, Hasegawa N, Iwata S, Hoshino Y
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Journal Title
Microbiology Resource Announcements
Volume: 8
Issue: 14
Pages: 0-0
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mycobacterium shigaense sp. nov., a slow-growing, scotochromogenic species, is a member of the Mycobacterium simiae complex2018
Author(s)
H. Fukano, M. Yoshida, Y. Kazumi, N. Fujiwara, K. Katayama, Y. Ogura, T. Hayashi, Y. Miyamoto, N. Fujimoto, W. Hongsheng, C. Mizumoto, Y. Koizumi, H. Maeda, O. Hiranuma, S. Mitarai, N. Ishii, and Y. Hoshino
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Journal Title
Int J Syst Evol Microbiol
Volume: 68(8)
Issue: 8
Pages: 2437-2442
DOI
Related Report
Peer Reviewed
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[Journal Article] Naturally occurring a loss of a giant plasmid from Mycobacterium ulcerans subsp. shinshuense makes it non-pathogenic2018
Author(s)
K. Nakanaga, Y. Ogura, A. Toyoda, M. Yoshida, H. Fukano, N. Fujiwara, Y. Miyamoto, N. Nakata, Y. Kazumi, S. Maeda, T. Ooka, M. Goto, K. Tanigawa, S. Mitarai, K. Suzuki, N. Ishii, M. Ato, T. Hayashi, and Y. Hoshino
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Journal Title
Sci Rep
Volume: 29;8(1)
Issue: 1
Pages: 8218-8218
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Clinical significance of interferon-γ neutralizing autoantibodies against disseminated nontuberculous mycobacterial disease.2018
Author(s)
Aoki A, Sakagami T, Yoshizawa K, Shima K, Toyama M, Tanabe Y, Moro H, Aoki N, Watanabe S, Koya T, Hasegawa T, Morimoto K, Kurashima A, Hoshino Y, Trapnell BC, Kikuchi T.
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Journal Title
Clin Infect Dis.
Volume: 66
Issue: 8
Pages: 1239-1245
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 肺Mycobacterium avium complex症に対する間欠的治療の検討 後ろ向き多施設共同研究2018
Author(s)
中川 拓, 朝倉 崇徳, 森本 耕三, 南宮 湖, 鈴木 翔二, 八木 光昭, 福井 保太, 林 悠太, 垂水 修, 山田 憲隆, 星野 仁彦, 倉島 篤行, 長谷川 直樹, 小川 賢二
Organizer
第93回日本結核病学会
Related Report
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