Frequency analysis of graft-versus-host reactive T cell clones in human acute graft-versus-host disease tissues
Project/Area Number |
18K08321
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 54010:Hematology and medical oncology-related
|
Research Institution | Nagoya University |
Principal Investigator |
MURATA Makoto 名古屋大学, 医学系研究科, 准教授 (40378063)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 造血幹細胞移植 / 移植片対宿主病 / T細胞 / T細胞受容体 / アロ抗原 |
Outline of Final Research Achievements |
The T cell repertoire in the skin tissues with acute graft-versus-host disease (GVHD) was more skewed when acute GVHD was severe or refractory. The frequent T cell clones in the GVHD skin tissues with a treatment failure were different from those in the GVHD skin tissues at diagnosis. Mixed culture of peripheral blood mononuclear cells that were obtained from pre-transplant recipient and donor could not identify the GVHD-associated T cell clones in the GVHD skin tissues.
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果はヒトの急性GVHDの病態解明に寄与するとともに、急性GVHDに対する新しい予防戦略、治療戦略の開発基盤になるものと期待される。例えば、生検皮膚組織を用いて急性GVHDの病理診断を行う際、T細胞浸潤や組織破壊の程度に加えて、TCRレパトアの偏りの強さも調べることで、GVHDの重症度をより正確に判定したり、その後の治療反応性を予測したりすることができる可能性がある。このような研究成果は、移植成績の向上、難治性血液疾患患者の予後改善につながるものと期待される。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] A multicenter phase II study of intrabone single-unit cord blood transplantation without antithymocyte globulin.2021
Author(s)
Nishida T, Kobayashi T, Sawa M, Masuda S, Shibasaki Y, Goto T, Fukuhara N, Fujii N, Ikegame K, Sugita J, Ikeda T, Kuwatsuka Y, Suzuki R, Najima Y, Doki N, Kato T, Inagaki Y, Utsu Y, Aotsuka N, Masuko M, Terakura S, Onishi Y, Maeda Y, Okada M, Teshima T, Murata M.
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Journal Title
Annals of Hematology
Volume: 100
Issue: 3
Pages: 743-752
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Allogeneic Hematopoietic Stem Cell Transplantation for Post-essential Thrombocythemia and Post-polycythemia Vera Myelofibrosis2020
Author(s)
Murata M, Suzuki R, Nishida T, Shirane S, Shimazu Y, Minami Y, Mori T, Doki N, Kanda Y, Uchida N, Tanaka M, Ishikawa J, Togitani K, Fukuda T, Ichinohe T, Atsuta Y, Nagamura-Inoue T, Kiyoi H.
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Journal Title
Internal Medicine
Volume: 59
Issue: 16
Pages: 1947-1956
DOI
NAID
ISSN
0918-2918, 1349-7235
Year and Date
2020-08-15
Related Report
Peer Reviewed / Open Access
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[Journal Article] Outcome of Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Myelodysplastic/Myeloproliferative Neoplasms-Unclassifiable: A Retrospective Nationwide Study of the Japan Society for Hematopoietic Cell Transplantation.2020
Author(s)
Kurosawa S, Shimomura Y, Tachibana T, Ishiyama K, Ota S, Kobayashi T, Uchida N, Fukushima K, Ashida T, Matsuoka K, Kanda J, Ichinohe T, Atsuta Y, Murata M, Aoki J.
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Journal Title
Biology of Blood and Marrow Transplantation
Volume: 26
Issue: 9
Pages: 1607-1611
DOI
Related Report
Peer Reviewed
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[Journal Article] Capturing Differential Allele-Level Expression and Genotypes of All Classical HLA Loci and Haplotypes by a New Capture RNA-Seq Method.2020
Author(s)
Yamamoto F, Suzuki S, Mizutani A, Shigenari A, Ito S, Kametani Y, Kato S, Fernandez-Vina M, Murata M, Morishima S, Morishima Y, Tanaka M, Kulski JK, Bahram S, Shiina T.
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Journal Title
Front Immunol.
Volume: 11
Pages: 941-941
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Prospective evaluation of alternative donor from unrelated donor and cord blood in adult acute leukemia and myelodysplastic syndrome2020
Author(s)
Terakura S, Nishida T, Sawa M, Kato T, Miyao K, Ozawa Y, Goto T, Kohno A, Ozeki K, Onishi Y, Fukuhara N, Fujii N, Yokoyama H, Kasai M, Iida H, Kanemura N, Endo T, Ago H, Onizuka M, Iyama S, Nawa Y, Nakamae M, Nagata Y, Kurahashi S, Tomiya Y, Yanagisawa A, Suzuki R, Kuwatsuka Y, Atsuta Y, Miyamura K, Murata M
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Journal Title
Bone Marrow Transplantation
Volume: --
Issue: 7
Pages: 1399-1409
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Donor single nucleotide polymorphism in ACAT1 affects the incidence of graft-versus-host disease after bone marrow transplantation.2020
Author(s)
Kamoshita S, Murata M, Koyama D, Julamanee J, Okuno S, Takagi E, Miyao K, Goto T, Ozawa Y, Miyamura K, Terakura S, Nishida T, Kiyoi H.
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Journal Title
International Journal of Hematology
Volume: 111
Issue: 1
Pages: 112-119
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Impact of homozygous conserved extended HLA haplotype on single cord blood transplantation: Lessons for induced pluripotent stem cell banking and transplantation in allogeneic settings.2020
Author(s)
Morishima Y, Morishima S, Murata M, Arima N, Uchida N, Sugio Y, Takahashi S, Matsuhashi Y, Onizuka M, Eto T, Nagafuji K, Onishi Y, Inoue M, Atsuta Y, Fukuda T, Ichinohe T, Kato S, Kanda J.
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Journal Title
Biology of Blood and Marrow Transplantation
Volume: 26
Issue: 1
Pages: 132-138
DOI
Related Report
Peer Reviewed
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[Journal Article] Prospective Phase 2 Study of Umbilical Cord Blood Transplantation in Adult Acute Leukemia and Myelodysplastic Syndrome2020
Author(s)
Terakura S, Nishida T, Sawa M, Kato T, Miyao K, Ozawa Y, Kohno A, Onishi Y, Fukuhara N, Kasai M, Fujii N, Yokoyama H, Iida H, Kanemura N, Fujieda A, Ago H, Tsutsumi Y, Nakamura F, Yago K, Moriuchi Y, Ota S, Ohashi H, Yanagisawa A, Suzuki R, Kuwatsuka Y, Atsuta Y, Miyamura K, Murata M
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Journal Title
Biology of Blood and Marrow Transplantation
Volume: 26
Issue: 1
Pages: 139-144
DOI
Related Report
Peer Reviewed
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[Journal Article] Analysis of glutathione S-transferase and cytochrome P450 gene polymorphism in recipients of dose-adjusted busulfan-cyclophosphamide conditioning2020
Author(s)
Terakura S, Onizuka M, Fukumoto M, Kuwatsuka Y, Kohno A, Ozawa Y, Miyamura K, Inagaki Y, Sawa M, Atsuta Y, Suzuki R, Naoe T, Morishita Y, Murata M
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Journal Title
Int J Hematol
Volume: 111
Issue: 1
Pages: 84-92
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparison of outcomes of allogeneic transplantation for primary myelofibrosis among hematopoietic stem cell source groups.2019
Author(s)
Murata M, Takenaka K, Uchida N, Ozawa Y, Ohashi K, Kim SW, Ikegame K, Kanda Y, Kobayashi H, Ishikawa J, Ago H, Hirokawa M, Fukuda T, Atsuta Y, Kondo T.
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Journal Title
Biology of Blood and Marrow Transplantation
Volume: 25
Issue: 8
Pages: 1536-1543
DOI
Related Report
Peer Reviewed
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[Journal Article] Quantitative Assessment of T Cell Clonotypes in Human Acute Graft-versus-Host Disease Tissues.2019
Author(s)
Koyama D, Murata M, Hanajiri R, Akashi T, Okuno S, Kamoshita S, Julamanee J, Takagi E, Miyao K, Sakemura R, Goto T, Terakura S, Nishida T, Kiyoi H
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Journal Title
Biol Blood Marrow Transplant
Volume: 25
Issue: 3
Pages: 417-423
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Impacts of thymoglobulin in patients with acute leukemia in remission undergoing allogeneic HSCT from different donors.2019
Author(s)
Wakamatsu M, Terakura S, Ohashi K, Fukuda T, Ozawa Y, Kanamori H, Sawa M, Uchida N, Ota S, Matsushita A, Kanda Y, Nakamae H, Ichinohe T, Kato K, Murata M, Atsuta Y, Teshima T.
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Journal Title
Blood Adv
Volume: 3
Issue: 2
Pages: 105-115
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Clinical implications of t(11;14) in patients with multiple myeloma undergoing autologous stem cell transplantation.2019
Author(s)
Takamatsu H, Yamashita T, Kurahashi S, Saitoh T, Kondo T, Maeda T, Nakazawa H, Murata M, Narita T, Kuroda J, Hashimoto H, Kawamura K, Miyamoto T, Honda S, Ichinohe T, Atsuta Y, Sunami K.
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Journal Title
Biol Blood Marrow Transplant
Volume: 25
Issue: 3
Pages: 474-479
DOI
Related Report
Peer Reviewed
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[Journal Article] Impact of graft-versus-host disease on relapse and survival after allogeneic stem cell transplantation for pediatric leukemia. Bone Marrow Transplant.2019
Author(s)
Kato M, Kurata M, Kanda J, Kato K, Tomizawa D, Kudo K, Yoshida N, Watanabe K, Shimada H, Inagaki J, Koh K, Goto H, Kato K, Cho Y, Yuza Y, Ogawa A, Okada K, Inoue M, Hashii Y, Teshima T, Murata M, Atsuta Y.
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Journal Title
Bone Marrow Transplant
Volume: 54
Issue: 1
Pages: 68-75
DOI
Related Report
Peer Reviewed
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[Journal Article] Introduction of genetically modified CD3ζ improves proliferation and persistence of antigen-specific CTLs.2018
Author(s)
Miyao K, Terakura S, Okuno S, Julamanee J, Watanabe K, Hamana H, Kishi H, Sakemura R, Koyama D, Goto T, Nishida T, Murata M, Kiyoi H.
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Journal Title
Cancer Immunol Res
Volume: 6
Issue: 6
Pages: 733-744
DOI
Related Report
Peer Reviewed
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[Presentation] Phase I study of cord blood transplantation with intra-bone marrow injection of MSC.2020
Author(s)
Goto T, Murata M, Nishida T, Terakura S, Kamoshita S, Ishikawa Y, Ushijima Y, Adachi Y, Kato K, Hirakawa A, Nishiwaki S, Nishio N, Takahashi Y, Kodera Y, Matsushita T, Kiyoi H.
Organizer
第82回日本血液学会学術集会
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[Presentation] Prospective evaluation of alternative donor from unrelated donor and cord blood in adult AL and MDS.2020
Author(s)
Goto T, Terakura S, Nishida T, Sawa M, Kato T, Miyao K, Ozawa Y, Kohno A, Ozeki K, Onishi Y, Fukuhara N, Fujii N, Yokoyama H, Kasai M, Iida H, Kanemura N, Endo T, Ago H, Onizuka M, Iyama S, Nagata Y, Kurahashi S, Yanagisawa A, Suzuki R, Kuwatsuka Y, Atsuta Y, Miyamura K, Murata M.
Organizer
第82回日本血液学会学術集会
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[Presentation] ヒト急性移植片対宿主病組織内高頻度T細胞クローンの経時的変化.2019
Author(s)
岡崎翔一郎, 村田 誠, 小山大輔, 堺 寿保, 牛島洋子, 鴨下園子, 奥野真吾, 高木えり奈, Jakrawadee Julamanee, 渡邊慶介, 今橋伸彦, 寺倉精太郎, 西田徹也, 清井 仁.
Organizer
第28回日本組織適合性学会大会
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[Presentation] 原発性骨髄線維症に対する同種造血幹細胞移植の成績:移植ソース間の比較.2019
Author(s)
村田 誠, 竹中克斗, 内田直之, 小澤幸泰, 大橋一輝, 金 成元, 池亀和博, 廣川 誠, 福田隆浩, 熱田由子, 近藤 健.
Organizer
第81回日本血液学会学術集会
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[Presentation] Phase I Study of Cord Blood Transplantation with Intra-Bone Marrow Injection of Mesenchymal Stem Cells.2019
Author(s)
Goto T, Murata M, Nishida T, Terakura S, Kamoshita S, Ishikawa Y, Ushijima Y, Adachi Y, Kato K, Hirakawa A, Nishiwaki S, Nishio N, Takahashi Y, Kodera Y, Matsushita T, Kiyoi H.
Organizer
The 61th Annual Meeting of the American Society of Hematology
Related Report
Int'l Joint Research
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[Presentation] Prospective Evaluation of Alternative Donor from Unrelated Volunteer Donor and Cord Blood in Adult Acute Leukemia and Myelodysplastic Syndrome: No Difference between Unrelated Donor and Cord Blood.2019
Author(s)
Terakura S, Nishida T, Sawa M, Kato T, Miyao K, Ozawa Y, Goto T, Kohno A, Ozeki K, Onishi Y, Fukuhara N, Fujii N, Yokoyama H, Kasai M, Iida H, Kanemura N, Endo T, Ago H, Onizuka M, Iyama S, Nawa Y, Nakamae M, Nagata Y, Kurahashi S, Tomiya Y, Yanagisawa A, Suzuki R, Kuwatsuka Y, Atsuta Y, Miyamura K, Murata M.
Organizer
The 61th Annual Meeting of the American Society of Hematology
Related Report
Int'l Joint Research
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[Presentation] The association between a SNP in the SOAT1 gene and the incidence of chronic GVHD after allo-HSCT.2018
Author(s)
Kamoshita S, Murata M, Koyama D, Sakai T, Okazaki S, Okuno S, Julamanee J, Takagi E, Miyao K, Goto T, Ozawa Y, Miyamura K, Terakura S, Nishida T, Kiyoi H.
Organizer
第80回日本血液学会学術集会
Related Report
-
[Presentation] Comparison of outcomes of allogeneic hematopoietic cell transplantation for primary myelofibrosis among stem cell source groups.2018
Author(s)
Murata M, Takenaka K, Uchida N, Ozawa Y, Ohashi K, Kim SW, Ikegame K, Atsuta Y, Hirokawa M, Fukuda T, Kondo T.
Organizer
The 60th annual meeting of the American Society of Hematology
Related Report
Int'l Joint Research