• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the mechanism of latent HTLV-1 infection for preventing from ATL onset of HTLV-1 carriers

Research Project

Project/Area Number 18K08378
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 54010:Hematology and medical oncology-related
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Hamaguchi Isao  国立感染症研究所, 血液・安全性研究部, 部長 (90348780)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsHTLV-1 / STLV-1 / 水平感染 / 胎盤感染 / マウスモデル / in situ hybridization法 / PVL / ヒト化マウス / In situ hybridization / in situ hybridization / 潜伏感染
Outline of Final Research Achievements

So far, we have established an HTLV-1 carrier mouse model that holds human blood cells. In addition, viral RNA signals were detected in the lymphatic tissues of infected mice by the next-generation in situ hybridization method.
Since the trophoblasts in the placenta revealed to be infected with HTLV-1, we administrated these cells into the abdominal cavity of humanized mice. The infection was established in mouse organisms, suggesting that the trophoblasts can be viral reservoirs and sources of infection. Furthermore, viral RNA was detected in the uterus and vagina of STLV-1 infected Japanese macaques suggested that they are considered useful as an animal model for elucidating the horizontal infection route of HTLV-1.

Academic Significance and Societal Importance of the Research Achievements

新しい解析技術を開発し、HTLV-1感染細胞が臓器のどの部分に存在するかを明らかにできた。HTLV-1水平感染や胎盤感染など、これから感染対策が行われる状況において、有用な成果につながった

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 8 results) Presentation (9 results) (of which Invited: 2 results)

  • [Journal Article] First case of molecularly identified and genetically characterized human T-cell leukemia virus type 2 infection in a pregnant woman in non-endemic Japan2021

    • Author(s)
      Kuramitsu Madoka、Okuma Kazu、Horiya Madoka、Sekizuka Tsuyoshi、Kaneko Noriaki、Saito Ema、Sokunaga Jun、Kuroda Makoto、Hamaguchi Isao
    • Journal Title

      Journal of Virological Methods

      Volume: 287 Pages: 114005-114005

    • DOI

      10.1016/j.jviromet.2020.114005

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Frequent horizontal and mother-to-child transmission may contribute to high prevalence of STLV-1 infection in Japanese macaques2020

    • Author(s)
      Murata Megumi、Yasunaga Jun-ichirou、Washizaki Ayaka、Seki Yohei、Kuramitsu Madoka、Tan Wei Keat、Hu Anna、Okuma Kazu、Hamaguchi Isao、Mizukami Takuo、Matsuoka Masao、Akari Hirofumi
    • Journal Title

      Retrovirology

      Volume: 17 Issue: 1 Pages: 1-10

    • DOI

      10.1186/s12977-020-00525-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of a novel diagnostic test algorithm for human T-cell leukemia virus type 1 infection with line immunoassay replacement of western blotting: a collaborative study for performance evaluation of diagnostic assays in Japan,2020

    • Author(s)
      Okuma K, Kuramitsu M, Niwa T, et al.(40人中17番目)
    • Journal Title

      Retrovirology

      Volume: 17 Issue: 1 Pages: 26-26

    • DOI

      10.1186/s12977-020-00534-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro Evaluation of the Safety of Adalimumab for the Eye Under HTLV-1 Infection Status: A Preliminary Study2020

    • Author(s)
      Kurozumi-Karube Hisako、Kamoi Koju、Ando Naoko、Uchida Minami、Hamaguchi Isao、Ohno-Matsui Kyoko
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 522579-522579

    • DOI

      10.3389/fmicb.2020.522579

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HTLV-1 targets human placental trophoblasts in seropositive pregnant women2020

    • Author(s)
      Tezuka Kenta、Fuchi Naoki、Okuma Kazu、Tsukiyama Takashi、Miura Shoko、Hasegawa Yuri、Nagata Ai、Komatsu Nahoko、Hasegawa Hiroo、Sasaki Daisuke、Sasaki Eita、Mizukami Takuo、Kuramitsu Madoka、Matsuoka Sahoko、Yanagihara Katsunori、Miura Kiyonori、Hamaguchi Isao
    • Journal Title

      Journal of Clinical Investigation

      Volume: 130 Issue: 11 Pages: 6171-6186

    • DOI

      10.1172/jci135525

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activation of PERK-ATF4-CHOP pathway as a novel therapeutic approach for efficient elimination of HTLV-1-infected cells2020

    • Author(s)
      Ikebe E, Matsuoka S, Tezuka K, Kuramitsu M, Okuma K, Nakashima M,, Seiichiro Kobayashi S, Makiyama J, Yamagishi M, Oyadomari S, Uchimaru K, Hamaguchi I
    • Journal Title

      Blood adv

      Volume: 4 Issue: 9 Pages: 1845-1858

    • DOI

      10.1182/bloodadvances.2019001139

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development and evaluation of human T-cell leukemia virus-1 and -2 multiplex quantitative PCR2019

    • Author(s)
      Kuramitsu M, Okuma K, Tezuka K, Nakamura H, Sagara Y, Kurane I, Hamaguchi I
    • Journal Title

      Microbiol. Immunol.

      Volume: 63 Issue: 1 Pages: 458-464

    • DOI

      10.1038/s41598-019-52686-5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adult T-cell leukemia/lymphoma-related ocular manifestations: analysis of the first large-scale nationwide survey2019

    • Author(s)
      Kamoi K, Okayama A, Izumo S, Hamaguchi I, Uchimaru K, Tojo A,Ohno-Matsui K
    • Journal Title

      Front Microbiol

      Volume: 9 Pages: 3240-3240

    • DOI

      10.3389/fmicb.2018.03240

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan2018

    • Author(s)
      Kuramitsu M、Okuma K、Nakashima M、Sato T、Sasaki D、Hasegawa H、Umeki K、Kubota R、Sasada K、Sobata R、Matsumoto C、Kaneko N、Tezuka K、Matsuoka S、Utsunomiya A、Koh KR、Ogata M、Ishitsuka K、Taki M、Nosaka K、Uchimaru K、Iwanaga M、Sagara Y、Yamano Y、Okayama A、Miura K et al
    • Journal Title

      Microbiology and Immunology

      Volume: 62 Issue: 10 Pages: 673-676

    • DOI

      10.1111/1348-0421.12644

    • NAID

      40021702629

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 1.小胞体ストレス応答を標的とした新規抗HTLV-1薬の開発2020

    • Author(s)
      池辺 詠美, 松岡 佐保子, 手塚 健太, 倉光 球, 大隈 和, 中島 誠, 小林 誠一郎, 牧山 純也, 山岸 誠, 親泊 政一, 内丸 薫, 浜口 功
    • Organizer
      第93 回日本生化学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] HTLV-1プロウイルス陽性のWB判定保留例に対するLIAの検討2019

    • Author(s)
      倉光球、大隈和、相良康子、中村仁美、手塚健太、浜口功
    • Organizer
      第6回日本HTLV-1学会
    • Related Report
      2019 Research-status Report
  • [Presentation] キャリア妊婦におけるHTLV-1経胎盤感染の実態解明の試み2019

    • Author(s)
      手塚健太、淵直樹、大隈和、築山尚史、長谷川ゆり、長谷川寛雄、佐々木大介、三浦生子、東島愛、佐々木永太、水上拓郎、倉光球、松岡佐保子、増﨑英明、三浦清徳、浜口功
    • Organizer
      第6回日本HTLV-1学会
    • Related Report
      2019 Research-status Report
  • [Presentation] HTLV-1検査法の改良と開発2019

    • Author(s)
      浜口功
    • Organizer
      第6回日本HTLV-1学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 血液製剤の安全性強化に向けたHTLV-1/2高感度マルチプレックス核酸検査法の開発2018

    • Author(s)
      大隈和、倉光球、手塚健太、倉根一郎、浜口功
    • Organizer
      日本輸血・細胞治療学会
    • Related Report
      2018 Research-status Report
  • [Presentation] HTLV-1定量PCRの標準化のための参照品の作成2018

    • Author(s)
      倉光球、大隈和、内丸薫、山野嘉久、長谷川寛雄、野坂生郷、岡山昭彦、久保田龍二、佐竹正博、金子典章、渡邉俊樹、浜口功
    • Organizer
      日本HTLV-1学会
    • Related Report
      2018 Research-status Report
  • [Presentation] HTLV-1/2マルチプレックス核酸検査法の開発と臨床検体を用いた評価2018

    • Author(s)
      大隈和、倉光球、手塚健太、中村仁美、相良康子、浜口功
    • Organizer
      日本HTLV-1学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 献血検体から見出された抗体陰性HTLV-1キャリア2018

    • Author(s)
      相良康子、中村仁美、倉光球、相良康弘、松永克希、平山秀明、島村益弘、岩永正子、大隈和、浜口功、入田和夫
    • Organizer
      日本HTLV-1学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Highly sensitive detection of plus- and minus-strand HTLV-1 mRNAs by RNA in situ hybridization in a humanized mouse model2018

    • Author(s)
      Tezuka K, Mizukami T, Sasaki E, Kuramitsu M, Matsuoka S, Okuma K, Hamaguchi I
    • Organizer
      日本ウイルス学会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi