| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Outline of Final Research Achievements |
Extracellular histones have been shown to induce endotoxicity to the vascular endothelial cells in severe inflammation such as sepsis. In this study, we found that extracellular vesicles (EVs) were released from human umbilical vein endothelial cells after histone addition. Since TNFα is known to release EVs from endothelial cells during inflammation, we compared the membrane protein of TNFα-derived and histone-derived EVs by proteomics analysis. We showed that the identified membrane proteins were useful markers for the evaluation of histone-induced endothelial injury. We also showed that EVs were formed around the plasma membrane by the electron microscopy, and histone aggregates on the plasma membrane by the immunoelectron microscopy. In addition, this EVs propagates the cytotoxicity to other endothelial cells.
|
