| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
The purpose of this study was to comprehensively and rapidly identify pathogens in clinical specimens obtained from patients with diseases of unknown etiology using metagenomic analysis. We also investigated the etiology of infectious diseases. However, the amount of pathogen-derived nucleic acids in clinical specimens is extremely low, and it is difficult to improve detection sensitivity, reduce costs, and shorten the time required for analysis. In this study, we developed novel techniques, such as a library preparation method for trace nucleic acids and a data analysis method to improve sensitivity for pathogen enrichment in clinical samples.
|
