Elucidation of the acid-sensitive mechanism and pathogenesis of the Group A Streptococcus spy1588 gene
| Project/Area Number |
18K08443
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Nagoya City University |
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Principal Investigator |
Isaka Masanori 名古屋市立大学, 医薬学総合研究院(医学), 助教 (40336673)
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| Co-Investigator(Kenkyū-buntansha) |
長谷川 忠男 名古屋市立大学, 医薬学総合研究院(医学), 教授 (10314014)
前山 順一 国立感染症研究所, 次世代生物学的製剤研究センター, 主任研究官 (40199641)
立野 一郎 名古屋市立大学, 医薬学総合研究院(医学), 講師 (50311642)
青山 峰芳 名古屋市立大学, 医薬学総合研究院(薬学), 教授 (70363918)
矢木 宏和 名古屋市立大学, 医薬学総合研究院(薬学), 准教授 (70565423)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | A群連鎖球菌 / 二成分制御因子 / ヒスチジンキナーゼ / バイオフィルム / 二成分制御系 / センサータンパク質 / 組換えタンパク質 / 二成分制御 / 立体構造 |
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| Outline of Final Research Achievements |
Group A Streptococcus produces biofilms in acidic environments. Biofilm is one of the virulence factors.The mechanism of this biofilm production has not yet been elucidated. The mechanism of pathogenesis of the fulminant form of this bacterium has also not yet been elucidated. The two-component regulatory system is a mechanism that sensitizes to external stimuli. We investigated the acid-sensing mechanism of SPY1588, which is involved in biofilm production in acidic environments, and the expression of virulence factors to investigate its relevance to the mechanism of the fulminant form. In this study, SPY1588 was involved in biofilm production under acidic conditions, SPY1588 recombinant protein autophosphorylated under acid conditions, and amino acid substitutions in the recombinant protein revealed the acid-sensitive site and kinase domain.
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| Academic Significance and Societal Importance of the Research Achievements |
A群連鎖球菌spy1588遺伝子並びにそのタンパク質SPY1588の酸感受性機構が明らかになると、この感染症の治療の主体である抗生物質投与から、体内の酸性代謝物の改善により、劇症型発症が抑えられることが期待される。現在でもこの感染症による劇症型発症が増加しているため、この結果を治療に反映したい。
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Report
(6 results)
Research Products
(25 results)
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[Journal Article] Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells.2022
Author(s)
Kondo K, Harada Y, Nakano M, Suzuki T, Fukushige T, Hanzawa K, Yagi H, Takagi K, Mizuno K, Miyamoto Y, Taniguchi N, Kato K, Kanekura T, Dohmae N, Machida K, Maruyama I, Inoue H.
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Journal Title
J Biol Chem.
Volume: 298
Pages: 101950-101950
Related Report
Peer Reviewed / Open Access
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[Journal Article] Lactone-Driven Ester-to-Amide Derivatization for Sialic Acid Linkage-Specific Alkylamidation2020
Author(s)
Jun-Ichi Furukawa 1, Hisatoshi Hanamatsu 2, Takashi Nishikaze 3, Hiroshi Manya 4, Nobuaki Miura 5, Hirokazu Yagi 6, Ikuko Yokota 1, Keiko Akasaka-Manya 4, Tamao Endo 4, Motoi Kanagawa 7 8, Norimasa Iwasaki 1, Koichi Tanaka 3
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Journal Title
Anal Chem .
Volume: 3
Issue: 21
Pages: 14383-14392
DOI
Related Report
Peer Reviewed / Open Access
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[Book] パワー・オブ・エイト2018
Author(s)
リン・マクタガート、島津 公美
Total Pages
384
Publisher
ダイヤモンド社
ISBN
9784478103364
Related Report
