| Project/Area Number |
18K08553
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Ohki Takeshi 東京女子医科大学, 医学部, 准教授 (90307562)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 再生医療 / 細胞シート / 縫合不全 / 腹膜 / ADSCシート |
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| Outline of Final Research Achievements |
The aim of this study was to develop a regenerative medicine rectal anastomosis augmentation therapy by creating a manipulatable hybrid cell sheet from peritoneal and adipose-derived stem cells (ASCs). Due to the coronal disaster, the large animal experiment was changed to a small animal one using rats. ASC sheets were prepared from rat subcutaneous fat and histologically compared between a group transplanted to the surface of the rat vastus lateralis muscle (muscle layer model) and a group transplanted with hybrid cell sheets (Experiment A). In addition, ASC sheets and hybrid cell sheets were transplanted into the surgically removed serosa of the stomach of rats as a gastrointestinal tract model (Experiment B). Hybrid cell sheets were successfully transplanted in small animals.
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| Academic Significance and Societal Importance of the Research Achievements |
縫合不全に対する再生医療的なアプローチは少なく細胞シートを用いた研究はない。腹膜は、術中に採取することが容易で、この腹膜を細胞シートのキャリアとして利用することで、軟らかく操作性の困難な細胞シートを吻合部の補強材料に使用できる可能性がある。本再生医療的治療法が確立すれば、直腸癌手術において人工肛門造設を減せる可能性がある。
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