| Project/Area Number |
18K08598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松浦 俊治 九州大学, 大学病院, 准教授 (10532856)
田口 智章 九州大学, 医学研究院, 教授 (20197247)
吉丸 耕一朗 九州大学, 医学研究院, 講師 (60711190)
山座 孝義 九州大学, 歯学研究院, 准教授 (80304814)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 歯髄幹細胞 / 肝再生医療 / SHED / 乳歯歯髄幹細胞 / 再生医療 / 臨床応用 / ゼノフリー / 乳歯歯髄バンク / Xeno-free / 肝不全 / ヒト乳歯歯髄細胞 / Xeno-free培地 |
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| Outline of Final Research Achievements |
We have developed a manufacturing process for isolating, culturing, and propagating multi-colony-derived SHEDs under XF/SF conditions, as well as a system for storing cells in two stages, MCB and WCB. We established quality control for the safety and quality (population doubling, chromosome stability, microbiological safety, and stem cell characteristics) of SHEDs as final products and SHEDs stored in MCBs and WCBs. In addition, we established a system to evaluate the in vivo immunogenicity and tumorigenicity of SHEDs as final products and stored products, as well as their liver regeneration effects using a mouse model of carbon tetrachloride-induced chronic liver fibrosis.
Translated with www.DeepL.com/Translator (free version)
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| Academic Significance and Societal Importance of the Research Achievements |
本研究でおこなった製造と品質検査によって得られたSHED最終製品は、慢性肝線維症の治療のために安全で有効な治療成績が示された。
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