Deployment to personalized medicine targeting pancreatic cancer stem cells and tumor microenvironment

• Project/Area Number: 18K08646
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Yamaguchi University
• Principal Investigator: Nagano Hiroaki 山口大学, 大学院医学系研究科, 教授 (10294050)
• Co-Investigator(Kenkyū-buntansha): 恒富 亮一 山口大学, 医学部附属病院, 講師 (10420514) ; 松隈 聰 山口大学, 医学部附属病院, 助教 (10634743) ; 徳光 幸生 山口大学, 医学部, 助教(寄附講座等) (40593299)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 癌幹細胞 / 膵癌 / 癌周辺微小環境 / 形質転換 / 腫瘍微小環境 / 個別化医療

Outline of Final Research Achievements

We have developed a method for inducing pancreatic cancer stem cell-like cells (P-CSLC) and reported that calreticulin (CRT) is a novel marker for cancer stem cells. In this study, we applied this result and identified a new therapeutic target potential for pancreatic cancer stem cells through a comprehensive approach of proteomics and mRNA analysis using a next-generation sequencer. In particular, we identified several types of molecules highly expressed in CRT-positive P-CSLC by a comprehensive approach and activated metabolic pathways in the same cells, and showed their potential as therapeutic targets. Furthermore, we were able to analyze the surface antigens associated with P-CSLC and cancer associated fibroblasts (CAFs).

Academic Significance and Societal Importance of the Research Achievements

本研究により解明された膵癌の治療抵抗性に関する知見は、癌幹細胞とも強く関連していると推察されるだけでなく、CAFや免疫逃避機構と癌幹細胞の関与についての可能性までをも追求してきた。
このことによる、膵癌の治療成績向上におけるこれらの機序解明の学術的意義は高い。

Research Products

• [Journal Article] Efficacy of intraoperative recurrent laryngeal neuromonitoring during surgery for esophageal cancer (2020)
  • Author(s): Takeda Shigeru、Iida Michihisa、Kanekiyo Shinsuke、Nishiyama Mitsuo、Tokumitsu Yukio、Shindo Yoshitaro、Yoshida Shin、Suzuki Nobuaki、Yoshino Shigefumi、Nagano Hiroaki
  • Journal Title: Annals of Gastroenterological Surgery Volume: 5 Issue: 1 Pages: 83-92
  • DOI: 10.1002/ags3.12394
  • Peer Reviewed
• [Journal Article] Clinical implications of cancer stem cells in digestive cancers: acquisition of stemness and prognostic impact. (2020)
  • Author(s): Tsunedomi R、Yoshimura K、Suzuki N、Hazama S、Nagano H
  • Journal Title: Surgery Today Volume: - Issue: 12 Pages: 1560-1577
  • DOI: 10.1007/s00595-020-01968-x
  • Peer Reviewed
• [Journal Article] The impact of peritoneal lavage cytology in biliary tract cancer (KHBO1701): Kansai Hepato‐Biliary Oncology Group (2020)
  • Author(s): Matsukuma Satoshi、Nagano Hiroaki、Ohmura Yoshiaki、Takeda Yutaka、Miyamoto Atsushi、Nakashima Shinsuke、Yamada Terumasa、Ajiki Tetsuo、Tsuji Akihito、Yoshimura Kenichi、Eguchi Hidetoshi、Hatano Etsuro、Ioka Tatsuya、Kansai Hepato‐Biliary Oncology (KHBO) Group
  • Journal Title: Cancer Reports Volume: 4 Issue: 2
  • DOI: 10.1002/cnr2.1323
  • Peer Reviewed
• [Journal Article] Risk prediction for malignant intraductal papillary mucinous neoplasm of the pancreas:logistic regression versus machine learning. (2020)
  • Author(s): Kang JS., Lee C., Song W., Cho W., Lee S., Han Y., Bassi C., Salvia R., Marchegiani G., Wolfgang CL., He J., Blair AB., Kluger MD., Su GH., Kim SC., Song KB., Yamamoto M., Higuchi R., Hatori T., Yang CY., Yamaue H., Hirono S., Satoi S., Fujii T., Hirano S., Lou W., Hashimoto Y., Shimizu Y etal.
  • Journal Title: Sci. Rep. Volume: 10 Issue: 1 Pages: 20140-20140
  • DOI: 10.1038/s41598-020-76974-7
  • Peer Reviewed / Open Access
• [Journal Article] Viral elimination is essential for improving surgical outcomes of hepatitis C virus‐related hepatocellular carcinoma: Multicenter retrospective analysis (2020)
  • Author(s): Nakajima Masao、Kobayashi Shogo、Wada Hiroshi、Tomokuni Akira、Takahashi Hidenori、Noda Takehiro、Matsui Hiroto、Matsukuma Satoshi、Kanekiyo Shinsuke、Shindo Yoshitaro、Tokumitsu Yukio、Nakagami Yuki、Suzuki Nobuaki、Takeda Shigeru、Tanabe Masahiro、Ito Katsuyoshi、Hoshii Yoshinobu、Eguchi Hidetoshi、Nagano Hiroaki
  • Journal Title: Annals of Gastroenterological Surgery Volume: 4 Issue: 6 Pages: 710-720
  • DOI: 10.1002/ags3.12377
  • Peer Reviewed
• [Journal Article] Multicenter International Society for Immunotherapy of Cancer Study of the Consensus Immunoscore for the Prediction of Survival and Response to Chemotherapy in Stage III Colon Cancer (2020)
  • Author(s): Mlecnik B., Kawakami Y, Galon J
  • Journal Title: Journal of Clinical Oncology Volume: 38 Issue: 31 Pages: 3638-3651
  • DOI: 10.1200/jco.19.03205
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Randomized clinical trial of pancreaticogastrostomy versus pancreaticojejunostomy regarding incidence of delayed gastric emptying after pancreaticoduodenectomy (2020)
  • Author(s): Eguchi Hidetoshi、Iwagami Yoshifumi、Matsushita Katsunori、Tomimaru Yoshito、Akita Hirofumi、Noda Takehiro、Gotoh Kunihito、Kobayashi Shogo、Nagano Hiroaki、Mori Masaki、Doki Yuichiro
  • Journal Title: Langenbeck's Archives of Surgery Volume: 405 Issue: 7 Pages: 921-928
  • DOI: 10.1007/s00423-020-01982-0
  • Peer Reviewed
• [Journal Article] Clinical outcomes of chemotherapy in patients with undifferentiated carcinoma of the pancreas: a retrospective multicenter cohort study (2020)
  • Author(s): Imaoka Hiroshi、Ikeda Masafumi、Maehara Kosuke、Umemoto Kumiko、Ozaka Masato、Kobayashi Satoshi、Terashima Takeshi、Inoue Hiroto、Sakaguchi Chihiro、Tsuji Kunihiro、Shioji Kazuhiko、Okamura Keiya、Kawamoto Yasuyuki、Suzuki Rei、Shirakawa Hirofumi、Nagano Hiroaki、Ueno Makoto、Morizane Chigusa、Furuse Junji
  • Journal Title: BMC Cancer Volume: 20 Issue: 1 Pages: 946-946
  • DOI: 10.1186/s12885-020-07462-4
  • Peer Reviewed
• [Journal Article] No Significant Effect of Daikenchuto (TJ-100) on Peritoneal IL-9 and IFN-γ Levels After Pancreaticoduodenectomy (2020)
  • Author(s): Maeda Hiromichi、Okada Ken-ichi、Fujii Tsutomu、Oba Mari S、Kawai Manabu、Hirono Seiko、Kodera Yasuhiro、Sho Masayuki、Akahori Takahiro、Shimizu Yasuhiro、Ambo Yoshiyasu、Kondo Naru、Murakami Yoshiaki、Ohuchida Jiro、Eguchi Hidetoshi、Nagano Hiroaki、Sakamoto Junichi、Yamaue Hiroki
  • Journal Title: Clinical and Experimental Gastroenterology Volume: Volume 13 Pages: 461-466
  • DOI: 10.2147/ceg.s262082
  • Peer Reviewed
• [Journal Article] A phase I study of multi-HLA-binding peptides derived from heat shock protein 70/glypican-3 and a novel combination adjuvant of hLAG-3Ig and Poly-ICLC for patients with metastatic gastrointestinal cancers: YNP01 trial. (2020)
  • Author(s): Masao;Nakajima, Shoichi, Hazama; Koji;Tamada. Nobuaki;Suzuki.Hiroaki;Nagano
  • Journal Title: Cancer Immunology, Immunotherapy Volume: 5 Issue: 8 Pages: 5-10
  • DOI: 10.1007/s00262-020-02518-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serum LOX-1 is a novel prognostic biomarker of colorectal cancer. (2020)
  • Author(s): Nakashima-Nakasuga C, Hazama S, Suzuki N, Nakagami Y, Xu M, Yoshida S, Tomochika S, Fujiwara N, Matsukuma S, Matsui H, Tokumitsu Y, Kanekiyo S, Shindo Y, Maeda N, Tsunedomi R, Iida M, Takeda S, Yoshino S, Ueno T, Hamamoto Y, Ogihara H, Hoshii Y, Nagano H.
  • Journal Title: Int J Clin Oncol. Volume: - Issue: 7 Pages: 1308-1317
  • DOI: 10.1007/s10147-020-01673-2
  • Peer Reviewed
• [Journal Article] The effect of the visceral fat area on the predictive accuracy of C‐reactive protein for infectious complications after laparoscopy‐assisted gastrectomy (2020)
  • Author(s): Iida Michihisa、Takeda Shigeru、Nakagami Yuki、Kanekiyo Shinsuke、Nakashima Chiyo、Nishiyama Mitsuo、Yoshida Shin、Suzuki Nobuaki、Yoshino Shigefumi、Nagano Hiroaki
  • Journal Title: Annals of Gastroenterological Surgery Volume: 4 Issue: 4 Pages: 386-395
  • DOI: 10.1002/ags3.12329
  • Peer Reviewed
• [Journal Article] COVID-19: clinical issues from the Japan Surgical Society (2020)
  • Author(s): Mori Masaki、Ikeda Norihiko、Taketomi Akinobu、Asahi Yo、Takesue Yoshio、Orimo Tatsuya、Ono Minoru、Kuwayama Takashi、Nakamura Seigo、Yamada Yohei、Kuroda Tatsuo、Yuzawa Kenji、Hibi Taizo、Nagano Hiroaki、Unno Michiaki、Kitagawa Yuko
  • Journal Title: Surgery Today Volume: 50 Issue: 8 Pages: 794-808
  • DOI: 10.1007/s00595-020-02047-x
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics of combined hepatocellular cholangiocarcinoma from the viewpoint of patient prognosis after hepatic resection: High rate of early recurrence and its predictors (2020)
  • Author(s): Yamashita Yo‐ichi、Aishima Shinichi、Nakao Yosuke、Yoshizumi Tomoharu、Nagano Hiroaki、Kuroki Tamotsu、Takami Yuko、Ide Takao、Ohta Masayuki、Takatsuki Mitsuhisa、Nanashima Atsushi、Ishii Fuminori、Kitahara Kenji、Iino Satoshi、Beppu Toru、Baba Hideo、Eguchi Susumu
  • Journal Title: Hepatology Research Volume: 50 Issue: 7 Pages: 863-870
  • DOI: 10.1111/hepr.13507
  • Peer Reviewed
• [Journal Article] Preoperative chemoradiotherapy using gemcitabine for pancreatic ductal adenocarcinoma in patients with impaired renal function (2020)
  • Author(s): Tomimaru Yoshito、Eguchi Hidetoshi、Iwagami Yoshifumi、Akita Hirofumi、Noda Takehiro、Gotoh Kunihito、Kobayashi Shogo、Nagano Hiroaki、Mori Masaki、Doki Yuichiro
  • Journal Title: Cancer Chemotherapy and Pharmacology Volume: 85 Issue: 3 Pages: 537-545
  • DOI: 10.1007/s00280-019-04005-y
  • Peer Reviewed
• [Journal Article] Novel Liquid Biopsy Test Based on a Sensitive Methylated SEPT9 Assay for Diagnosing Hepatocellular Carcinoma. Hepatol Commun (2020)
  • Author(s): Kotoh Y, Suehiro Y, Saeki I, Hoshida T, Maeda M, Iwamoto T, Matsumoto T, Hidaka I, Ishikawa T, Takami T, Higaki S, Fujii I, Suzuki C, Shindo Y, Tokumitsu Y, Nagano H, Sakaida I, Yamasaki T
  • Journal Title: Hepatol Commun Volume: 4(3) Issue: 3 Pages: 461-470
  • DOI: 10.1002/hep4.1469
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model (2020)
  • Author(s): Elbadawy Mohamed, Yamanaka Megumi, Goto Yuta,Hayashi Kimika,Tsunedomi Ryouichi,Hazama Shoichi,Nagano Hiroaki,Yoshida Toshinori,Shibutani Makoto,Ichikawa Ryo,Nakahara Junta,Omatsu Tsutomu,Mizutani Tetsuya,Katayama Yukie,Shinohara Yuta,Abugomaa Amira,Kaneda Masahiro,Yamawaki Hideyuki,Usui Tatsuya,Sasaki Kazuaki
  • Journal Title: Biomaterials Volume: 237 Pages: 119823-119823
  • DOI: 10.1016/j.biomaterials.2020.119823
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A prospective, multi-center Phase I study of postoperative enoxaparin treatment in patients undergoing curative hepatobiliary-pancreatic surgery for malignancies (2020)
  • Author(s): Eguchi H, Kawamoto K, Tsujie M, Yukawa M, Kubota M, Asaoka T, Takeda Y, Noda T, Shimizu J, Nagano H, Doki Y, Mori M
  • Journal Title: Dig Surg Volume: 37(1) Issue: 1 Pages: 81-86
  • DOI: 10.1159/000497451
  • Peer Reviewed
• [Journal Article] “Logic-based medicine” is more feasible than “evidence-based medicine” in the local treatment for hepatocellular carcinoma (2020)
  • Author(s): Sakon M, Kobayashi S, Wada H, Eguchi H, Marubashi S, Takahashi H, Akita H, Gotoh K, Yamada D, Asukai K, Hasegawa S, Ohue M, Yano M, Nagano H
  • Journal Title: Oncology Volume: 98(5) Issue: 5 Pages: 259-266
  • DOI: 10.1159/000505554
  • Peer Reviewed
• [Journal Article] The time has come for Japanese surgeons to work in English (2020)
  • Author(s): Nagano Hiroaki
  • Journal Title: Annals of Gastroenterological Surgery Volume: 4 Issue: 1 Pages: 5-5
  • DOI: 10.1002/ags3.12313
  • Peer Reviewed
• [Journal Article] Fecal DNA Testing of TWIST1 Methylation Identifies Patients With Advanced Colorectal Adenoma Missed by Fecal Immunochemical Test for Hemoglobin (2020)
  • Author(s): Suehiro Y, Hashimoto S, Goto A, Yamaoka Y, Yamamoto N, Suzuki N, Higaki S, Fujii I, Suzuki C, Matsumoto T, Hoshida T, Koga M, Tsutsumi T, Lim LA, Matsubara Y, Takami T, Yoshida S, Hazama S, Yotsuyanagi H, Nagano H, Sakaida I, Yamasaki T.
  • Journal Title: Clinical and Translational Gastroenterology Volume: 11 Issue: 6 Pages: e00176-e00176
  • DOI: 10.14309/ctg.0000000000000176
  • Peer Reviewed / Open Access
• [Journal Article] Surgical Outcomes of Hepatocellular Carcinoma With Bile Duct Tumor Thrombus (2020)
  • Author(s): Kim DS, Kim BW, Hatano E, Nagano H, Yamasaki O, Yu HC, Han DH, Seo HI, Park IY, Yang KS, Yamamoto M, Wang HJ
  • Journal Title: Annals of Surgery Volume: 271 Issue: 5 Pages: 913-921
  • DOI: 10.1097/sla.0000000000003014
  • Peer Reviewed
• [Journal Article] Laparoscopic total biopsy for suspected gallbladder cancer: A case series (2020)
  • Author(s): Tokumitsu Yukio、Shindo Yoshitaro、Matsui Hiroto、Matsukuma Satoshi、Nakajima Masao、Yoshida Shin、Iida Michihisa、Suzuki Nobuaki、Takeda Shigeru、Nagano Hiroaki
  • Journal Title: Health Science Reports Volume: 3(2) Issue: 2
  • DOI: 10.1002/hsr2.156
  • Peer Reviewed
• [Journal Article] Liver resection with thrombectomy improves the survival of patients with hepatocellular carcinoma and tumor thrombi in the inferior vena cava or right atrium (2020)
  • Author(s): Matsukuma S, Eguchi H, Wada H, Noda T, Shindo Y, Tokumitsu Y, Matsui H, Takahashi H, Kobayashi S, Nagano H
  • Journal Title: BJS Open Volume: 4(2) Pages: 241-251
  • Peer Reviewed / Open Access
• [Journal Article] TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients (2019)
  • Author(s): Matsuda T, Miyauchi E, Hsu YW, Nagayama S, Kiyotani K, Zewde M, Park JH, Kato T, Harada M, Matsui S, Ueno M, Fukuda K, Suzuki N, Hazama S, Nagano H, Takeuchi H, Vigneswaran WT, Kitagawa Y, Nakamura Y
  • Journal Title: Oncoimmunology Volume: 8(6) Issue: 6 Pages: e1588085-e1588085
  • DOI: 10.1080/2162402x.2019.1588085
  • Peer Reviewed
• [Journal Article] A double-blind randomized comparative clinical trial to evaluate the safety and efficacy of dendritic cell vaccine loaded with WT1 peptides (TLP0-001) in combination with S-1 in patients with advanced pancreatic cancer refractory to standard chemotherapy. (2019)
  • Author(s): Katsuda M, Miyazawa M, Ojima T, Katanuma A, Hakamada K, Sudo K, Asahara S, Endo I, Ueno M, Hara K, Yamada S, Fujii T, Satoi S, Ioka T, Ohira M, Akahori T, Kitano M, Nagano H, Furukawa M, Adachi T, Yamaue H.
  • Journal Title: Trials Volume: 20 Issue: 1 Pages: 242-242
  • DOI: 10.1186/s13063-019-3332-5
  • Peer Reviewed / Open Access
• [Journal Article] A Prospective, Randomized Phase II Study of Adjuvant Gemcitabine Versus S-1 After Major Hepatectomy for Biliary Tract Cancer (KHBO 1208): Kansai Hepato-Biliary Oncology Group (2019)
  • Author(s): Kobayashi S, Nagano H, Tomokuni A, Gotoh K, Sakai D, Hatano E, Seo S, Terajima H, Uchida Y, Ajiki T, Satake H, Kamei K, Tohyama T, Hirose T, Ikai I, Morita S, Ioka T; Kansai Hepato-Biliary Oncology (KHBO) Group
  • Journal Title: Ann Surg Volume: 270(2) Issue: 2 Pages: 230-237
  • DOI: 10.1097/sla.0000000000002865
  • Peer Reviewed
• [Journal Article] Essential updates to the surgical treatment of biliary tract cancer (2019)
  • Author(s): Matsukuma S, Tokumitsu Y, Shindo Y, Matsui H, Nagano H
  • Journal Title: Ann Gastroenterol Surg Volume: 3(4) Issue: 4 Pages: 378-389
  • DOI: 10.1002/ags3.12266
  • Peer Reviewed
• [Journal Article] Identification of a Promiscuous Epitope Peptide Derived From HSP70. (2019)
  • Author(s): Matsui H, Hazama S, Tamada K, Udaka K, Irie A, Nishimura Y, Miyakawa T, Doi S, Nakajima M, Kanekiyo S, Tokumitsu Y, Shindo Y, Tomochika S, Yoshida S, Iida M, Suzuki N, Takeda S, Yamamoto S, Yoshino S, Ueno T, Nagano H
  • Journal Title: J Immunother Volume: 42 Issue: 7 Pages: 244-250
  • DOI: 10.1097/cji.0000000000000274
  • Peer Reviewed / Open Access
• [Journal Article] Novel Biomarkers for Personalized Cancer Immunotherapy. (2019)
  • Author(s): Shindo Y, Hazama S, Tsunedomi R, Suzuki N, Nagano H.
  • Journal Title: Cancers (Basel). Volume: 121 Issue: 9 Pages: 983-984
  • DOI: 10.3390/cancers11091223
  • Peer Reviewed / Open Access
• [Journal Article] Utility of scoring systems combining the product of tumor number and size with liver function for predicting the prognosis of patients with hepatocellular carcinoma after hepatectomy (2019)
  • Author(s): Tokumitsu Y, Shindo Y, Matsui H, Matsukuma S, Nakajima M, Suzuki N, Takeda S, Wada H, Kobayashi S, Eguchi H, Ueno T, Nagano H
  • Journal Title: Oncol Lett Volume: 18(4) Pages: 3903-3913
  • DOI: 10.3892/ol.2019.10688
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Improved survival of CAR-T and tumor-specific T cells caused by anti-PD-1 scFv-producing CAR-T cells (2019)
  • Author(s): Nakajima M, Sakoda Y, Adachi K, Nagano H, Tamada K
  • Journal Title: Cancer Sci Volume: 110(10) Issue: 10 Pages: 3079-3088
  • DOI: 10.1111/cas.14169
  • Peer Reviewed
• [Journal Article] Intratumoural-infiltrating CD4+ and FOXP3+ T Cells as strong positive predictive markers for the prognosis of resectable colorectal cancer (2019)
  • Author(s): Kuwahara T, Hazama S, Suzuki N, Yoshida S, Tomochika S, Nakagami Y, Matsui H, Shindo Y, Kanekiyo S, Tokumitsu Y, Iida M, Tsunedomi R, Takeda S, Yoshino S, Okayama N, Suehiro Y, Yamasaki T, Fijita T, Kawakami Y, Ueno T, Nagano H
  • Journal Title: Br J Cancer Volume: 121(11) Issue: 8 Pages: 983-984
  • DOI: 10.1038/s41416-019-0559-6
  • Peer Reviewed
• [Journal Article] Genomic profiles of colorectal carcinoma with liver metastases and newly identified fusion genes (2019)
  • Author(s): Oga T, Yamashita Y, Soda M, Kojima S, Ueno T, Kawazu M, Suzuki N, Nagano H, Hazama S, Izumiya M, Koike K, Mano H
  • Journal Title: Cancer Sci Volume: 110(9) Issue: 9 Pages: 2973-2981
  • DOI: 10.1111/cas.14127
  • Peer Reviewed
• [Journal Article] Multicenter phase II study of biweekly XELIRI plus bevacizumab as second-line therapy in patients with metastatic colorectal cancer (JSWOG-C3 study) (2019)
  • Author(s): Suzuki N, Hazama S, Nagasaka T, Tanioka H, Iwamoto Y, Negoro Y, Yamauchi M, Kobayashi M, Okuda H, Fujishima N, Nishimura T, Yamanaka N, Toyota K, Mori Y, Nakagami Y, Shimokawa M, Nagano H, Okajima M
  • Journal Title: Int J Clin Oncol Volume: 24(10) Issue: 10 Pages: 1223-1230
  • DOI: 10.1007/s10147-019-01473-3
  • Peer Reviewed / Open Access
• [Journal Article] A Prospective, Open-Label, Multicenter Phase 2 Trial of Neoadjuvant Therapy Using Full-Dose Gemcitabine and S-1 Concurrent with Radiation for Resectable Pancreatic Ductal Adenocarcinoma (2019)
  • Author(s): Eguchi H, Takeda Y, Takahashi H, Nakahira S, Kashiwazaki M, Shimizu J, Sakai D, Isohashi F, Nagano H, Mori M, Doki Y
  • Journal Title: Ann Surg Oncol Volume: 26(13) Issue: 13 Pages: 4498-4505
  • DOI: 10.1245/s10434-019-07735-8
  • Peer Reviewed
• [Journal Article] Clinical Practice Guidelines for Hepatocellular Carcinoma: The Japan Society of Hepatology 2017 (4th JSH-HCC Guidelines) a 2019 update (2019)
  • Author(s): Kokudo N, Takemura N, Hasegawa K, Takayama T, Kubo S, Shimada M, Nagano H, Hatano E, Izumi N, Kaneko S, Kudo M, Iijima H, Genda T, Tateishi R, Torimura T, Igaki H, Kobayashi S, Sakurai H, Murakami T, Watadani T, Matsuyama Y
  • Journal Title: Hepatol Res Volume: 49(10) Issue: 10 Pages: 1109-1113
  • DOI: 10.1111/hepr.13411
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Objective Response by mRECIST Is an Independent Prognostic Factor for Overall Survival in Hepatocellular Carcinoma Treated with Sorafenib in the SILIUS Trial (2019)
  • Author(s): Kudo M, Ueshima K, Chiba Y, Ogasawara S, Obi S, Izumi N, Aikata H, Nagano H, Hatano E, Sasaki Y, Hino K, Kumada T, Yamamoto K, Imai Y, Iwadou S, Ogawa C, Okusaka T, Kanai F, Arai Y
  • Journal Title: Liver Cancer Volume: 8(6) Issue: 6 Pages: 505-519
  • DOI: 10.1159/000503032
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a Screening Method for Epstein-Barr Virus-Associated Gastric Carcinoma by Droplet Digital PCR. (2019)
  • Author(s): Shuto T, Nishikawa J, Shimokuri K, Yanagi A, Takagi T, Takagi F, Miura O, Iida M, Nagano H, Takemoto Y, Harada E, Suehiro Y, Yamasaki T, Okamoto T, Sakaida I.
  • Journal Title: Microorganisms Volume: 7(12) Issue: 12 Pages: 628-628
  • DOI: 10.3390/microorganisms7120628
  • Peer Reviewed / Open Access
• [Journal Article] Association between polymorphisms in EGFR and tumor response during cetuximab and oxaliplatin-based combination therapy in metastatic colorectal cancer: Analysis of data from two clinical trials (2019)
  • Author(s): Maeda H, Hazama S, Iwamoto S, Oba K, Tsunedomi R, Okayama N, Suehiro Y, Yamasaki T, Nakagami Y, Suzuki N, Nagano H, Sakamoto J, Mishima H, Nagata N
  • Journal Title: Oncol Lett Volume: 18(5) Pages: 4555-4562
  • DOI: 10.3892/ol.2019.10787
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy (2018)
  • Author(s): Kamei R, Yoshimura K, Yoshino S, Inoue M, Asao T, Fuse M, Wada S, Kuramasu A, Furuya-Kondo T, Oga A, Iizuka N, Suzuki N, Maeda N, Watanabe Y, Matsukuma S, Iida M, Takeda S, Ueno T, Yamamoto N, Fukagawa T, Katai H, Sasaki H, Hazama S, Oka M, Nagano H.
  • Journal Title: Oncology Letters Volume: 15 Pages: 747-754
  • DOI: 10.3892/ol.2017.7354
  • Peer Reviewed / Open Access
• [Journal Article] A new prognostic model for hepatocellular carcinoma recurrence after curative hepatectomy (2018)
  • Author(s): Tokumitsu Yukio、Sakamoto Kazuhiko、Tokuhisa Yoshihiro、Matsui Hiroto、Matsukuma Satoshi、Maeda Yoshinari、Sakata Koichiro、Wada Hiroshi、Eguchi Hidetoshi、Ogihara Hiroyuki、Fujita Yusuke、Hamamoto Yoshihiko、Iizuka Norio、Ueno Tomio、Nagano Hiroaki
  • Journal Title: Oncology Letters Volume: 15(4) Pages: 4411-4422
  • DOI: 10.3892/ol.2018.7821
  • Peer Reviewed / Open Access
• [Journal Article] Low invasiveness of thoracoscopic esophagectomy in the prone position for esophageal cancer: a propensity score-matched comparison of operative approaches between thoracoscopic and open esophagectomy. (2018)
  • Author(s): Shinsuke Kanekiyo, Shigeru Takeda, Shigefumi Yoshino, Hiroaki Nagano
  • Journal Title: Surg Endosc. Volume: 32(4) Issue: 4 Pages: 1945-1953
  • DOI: 10.1007/s00464-017-5888-z
  • Peer Reviewed
• [Journal Article] Metastatic ability and the epithelial-mesenchymal transition in induced cancer stem-like hepatoma cells (2018)
  • Author(s): Nishiyama M、Tsunedomi R、Yoshimura K、Hashimoto Ni、Matsukuma S、Ogihara H、Kanekiyo S、Iida M、Sakamoto K、Suzuki N、Takeda S、Yamamoto S、Yoshino S、Ueno T、Hamamoto Y、Hazama S、Nagano H
  • Journal Title: Cancer Science Volume: 109 Issue: 4 Pages: 1101-1109
  • DOI: 10.1111/cas.13527
  • Peer Reviewed / Open Access
• [Journal Article] IgG response to MHC class I epitope peptides is a quantitative predictive biomarker in the early course of treatment of colorectal cancer using therapeutic peptides (2018)
  • Author(s): Kanekiyo S, Hazama S, Takenouchi H, Nakajima M, Shindo Y, Matsui H, Tokumitsu Y, Tomochika S, Tsunedomi R, Tokuhisa Y, Iida M, Sakamoto K, Suzuki N, Takeda S, Yamamoto S, Yoshino S, Okuno K, Udaka K, Kawakami Y, Matsueda S, Ito K, Nagano H.
  • Journal Title: Oncology Reports Volume: 39 Pages: 2385-2392
  • DOI: 10.3892/or.2018.6288
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of miR221 and miR222 in the cancer stroma is associated with malignant potential in colorectal cancer (2018)
  • Author(s): Iida Michihisa、Hazama Shoichi、Tsunedomi Ryouichi、Tanaka Hironori、Takenouchi Hiroko、Kanekiyo Shinsuke、Tokumitsu Yukio、Tomochika Shinobu、Tokuhisa Yoshihiro、Sakamoto Kazuhiko、Suzuki Nobuaki、Takeda Shigeru、Ueno Tomio、Yamamoto Shigeru、Yoshino Shigefumi、Fujita Koji、Kuroda Masahiko、Nagano Hiroaki
  • Journal Title: Oncology Reports Volume: 40 Pages: 1621-1631
  • DOI: 10.3892/or.2018.6575
  • Peer Reviewed / Open Access
• [Journal Article] Outcomes following liver resection for multinodular Barcelona Clinic Liver Cancer?B hepatocellular carcinoma (2018)
  • Author(s): Matsukuma Satoshi、Sakamoto Kazuhiko、Tokuhisa Yoshihiro、Tokumitsu Yukio、Matsui Hiroto、Kanekiyo Shinsuke、Tomochika Shinobu、Iida Michihisa、Suzuki Nobuaki、Takeda Shigeru、Ueno Tomio、Wada Hiroshi、Kobayashi Shogo、Saeki Issei、Eguchi Hidetoshi、Sakon Masato、Sakaida Isao、Nagano Hiroaki
  • Journal Title: Oncology Letters Volume: 16(5) Pages: 6383-6392
  • DOI: 10.3892/ol.2018.9420
  • Peer Reviewed / Open Access
• [Journal Article] Combination treatment of advanced pancreatic cancer using novel vaccine and traditional therapies (2018)
  • Author(s): Matsui H、Hazama S、Shindo Y、Nagano H
  • Journal Title: Expert Review of Anticancer Therapy Volume: 18 Issue: 12 Pages: 1205-1217
  • DOI: 10.1080/14737140.2018.1531707
  • Peer Reviewed
• [Journal Article] Current status of immunotherapy against gastrointestinal cancers and its biomarkers: Perspective for precision immunotherapy (2018)
  • Author(s): Hazama S、Tamada K、Yamaguchi Y、Kawakami Y、Nagano H
  • Journal Title: Annals of Gastroenterological Surgery Volume: 2 Issue: 4 Pages: 289-303
  • DOI: 10.1002/ags3.12180
  • Peer Reviewed / Open Access
• [Journal Article] High secreted protein acidic and rich in cysteine expression in peritumoral fibroblasts predicts better prognosis in patients with resectable gastric cancer (2017)
  • Author(s): Nakajima Masao、Yoshino Shigefumi、Kanekiyo Shinsuke、Maeda Noriko、Sakamoto Kazuhiko、Tsunedomi Ryoichi、Suzuki Nobuaki、Takeda Shigeru、Yamamoto Shigeru、Hazama Shoichi、Hoshii Yoshinobu、Oga Atsunori、Itoh Hiroshi、Ueno Tomio、Nagano Hiroaki
  • Journal Title: Oncology Letters Volume: 15 Pages: 803-812
  • DOI: 10.3892/ol.2017.7418
  • Peer Reviewed / Open Access
• [Presentation] Induction of antigen specific CD8+T cell infiltration by a novel neoadjuvant vaccine containing HSP70 and GPC3 peptides plus soluble LAG-3 and Poly-IC: LC : Interim results of a Phase I study (2019)
  • Author(s): Tokumitsu Y, Hazama S, Doi S, Tamada K, Udaka K,Akinaga S, Koki Y, Uematsu T, Nakagami Y, Matsui H,Shindo Y, Kanekiyo S, Tomochika S, Iida M, Yoshida S,Suzuki N, Takeda S, Yoshino S, Ueno T, Nagano H
  • Organizer: 2019 ASCO Annual Meeting
  • Int'l Joint Research
• [Presentation] Cancer stem-like phenotypes including immune surveillance and its responsible genes in induced liver cancer stem-like cells. Poster (2019)
  • Author(s): Tsunedomi R, Yoshimura K, Kimura Y, Nishiyama M,Matsukuma S, Tokumitsu Y, Tomochika S, Iida M, Suzuki N, Takeda S, Yoshino S, Hazama S, Nagano H
  • Organizer: ESMO Asia Congress
  • Int'l Joint Research
• [Presentation] Analysis of T cells of HCC patients: From a phase I study of novel neoadjuvant vaccine PBMCs and TILs (2019)
  • Author(s): Nakagami Y, Hazama S, Doi S, Tamada K, Udaka K,Akinaga S, Adachi K, Ming Xu, Tokumitsu Y, Matsui H,Shindo Y, Tsunedomi R, Kanekiyo S, Tomochika S, Iida M,Yoshida S, Suzuki N, Takeda S, Yoshino S, Nagano H
  • Organizer: 7th International Conference of Federation of Asian Clinical Oncology(FACO)
  • Int'l Joint Research
• [Presentation] 肝転移能亢進を示す肝癌幹細胞におけるRAB3Bの特性 Characteristics of RAB3B in hepatoma stem-like sphere cells (2019)
  • Author(s): 恒富亮一、吉村 清、木村祐太、西山光郎、松隈 聰、新藤芳太郎、徳光幸生、友近 忍、吉田 晋、飯田通久、鈴木伸明、 武田 茂、山本 滋、吉野茂文、硲 彰一、上野富雄、永野浩昭
  • Organizer: 第28回日本がん転移学会学術集会・総会
• [Presentation] HCCに対する新規ネオアジュバントワクチン療法の第I相・試験－TILとPBMC解析－A phase I study of novel neoadjuvant vaccine for HCC: Interim analysis of TILs and PBMCs (2019)
  • Author(s): 中上裕有樹、硲 彰一、土肥 俊、玉田耕治、宇高恵子、徳光幸生、松井洋人、松隈 聰、新藤芳太郎、鈴木伸明、武田 茂、 永野浩昭
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] 肝癌幹細胞様 Sphere 細胞において同定された RAB3B 遺伝子の役割 (2019)
  • Author(s): 恒富亮一、吉村清、松隈聰、飯田通久、鈴木伸明、武田茂、吉野茂文、硲彰一、上野富雄、永野浩昭
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] First in human Phase I study of AI designed novel vaccination therapy for the patients with advanced solid tumors (2019)
  • Author(s): 中島正夫、硲 彰一、玉田耕治、宇高恵子、土肥 俊、松井洋人、松隈 聰、新藤芳太郎、徳光幸生、兼清信介、友近 忍、 吉田 晋、飯田通久、鈴木伸明、武田 茂、吉野茂文、上野富雄、永野浩昭
  • Organizer: 第23回日本がん免疫学会総会
• [Presentation] Induction of antigen specific CD8 T cell infiltration by a novel neoadjuvant vaccine： Interim results of a Phase I study (2019)
  • Author(s): 中上裕有樹、硲 彰一、土肥 俊、玉田耕治、宇高恵子、秋永士朗、構木泰信、植松俊成、徳光幸生、松井洋人、新藤芳太郎、 兼清信介、友近 忍、飯田通久、吉田 晋、鈴木伸明、武田 茂、吉野茂文、上野富雄、永野浩昭
  • Organizer: 第23回日本がん免疫学会総会
• [Presentation] ネオアンチゲンを標的としたがんワクチン療法の開発 (2019)
  • Author(s): 新藤芳太郎、硲 彰一、恒富亮一、徳光幸生、松井洋人、松隈 聰、兼清信介、友近 忍、吉田 晋、飯田通久、鈴木伸明、 武田 茂、吉野茂文、玉田耕治、永野浩昭
  • Organizer: 第57回日本癌治療学会学術集会
• [Presentation] 膵癌に対する術前化学療法の経験 (2019)
  • Author(s): 藤原康弘、新藤芳太郎、徳光幸生、松井洋人、松隈 聰、中島正夫、兼清信介、友近 忍、吉田 晋、飯田通久、鈴木伸明、 武田 茂、硲 彰一、上野富雄、永野浩昭
  • Organizer: 第57回日本癌治療学会学術集会
• [Presentation] 消化器癌に対する新規ペプチドワクチン療法第1相試験 (2019)
  • Author(s): 中島正夫、硲 彰一、玉田耕治、宇高恵子、土肥 俊、松井洋人、新藤芳太郎、徳光幸生、松隈 聰、友近 忍、飯田通久、 吉田 晋、鈴木伸明、武田 茂、永野浩昭
  • Organizer: 第57回日本癌治療学会学術集会
• [Presentation] 当科における切除不能膵癌に対する conversion surgery の治療成績 (2019)
  • Author(s): 新藤芳太郎、徳光幸生、松井洋人、中島正夫、松隈 聰、兼清信介、友近 忍、吉田 晋、飯田通久、鈴木伸明、武田 茂、 吉野茂文、硲 彰一、上野富雄、永野浩昭
  • Organizer: JDDW 2019 第27回日本消化器関連学会週間
• [Presentation] 膵癌術前化学療法が患者免疫学的プロファイルに与える影 響の検討 (2019)
  • Author(s): 中島正夫、硲 彰一、山本常則、山田健介、中上裕有樹、徳光幸生、松井洋人、新藤芳太郎、松隈 聰、兼清信介、友近 忍、 吉田 晋、飯田通久、鈴木伸明、武田 茂、吉野茂文、永野浩昭
  • Organizer: 第32回日本バイオセラピィ学会学術集会総会
• [Presentation] 新規がんペプチド 複合免疫療法の開発 (2019)
  • Author(s): 硲 彰一、鈴木伸明、吉田 晋、友近 忍、徳光幸生、新藤芳太郎、松井洋人、松隈 聰、中島正夫、中島千代、山本常則、 千々松日香里、中上裕有樹、武田 茂、飯田通久、兼清信介、玉田耕治、宇高恵子、吉野茂文、永野浩昭
  • Organizer: 第32回日本バイオセラピィ学会学術集会総会
• [Presentation] がん幹細胞様細胞における CD44 isoforms 発現 (2018)
  • Author(s): 恒富亮一、西山光郎、松隈聰、鈴木伸明、武田茂、山本滋、吉野茂文、硲彰一、上野富雄、永野浩昭
  • Organizer: 第6回がんと代謝研究会
• [Presentation] 肝癌細胞株から誘導した癌幹細胞の転移能解析 (2018)
  • Author(s): 松隈聰、恒富亮一、西山光郎、松井洋人、徳光幸生、徳久善弘、坂本和彦、兼清信介、友近忍、飯田通久、鈴木伸明、武田茂、吉野茂文、硲彰一、上野富雄、吉村清、永野浩昭
  • Organizer: 第54回日本肝癌研究会
• [Presentation] 肝細胞癌幹細胞様 Sphere 細胞における抗癌剤耐性に関わるRAB3B遺伝子 (2018)
  • Author(s): 恒富亮一、吉村清、松隈聰、藤原信行、西山光郎、兼清信介、飯田通久、鈴木伸明、武田茂、吉野茂文、硲彰一、上野富雄、永野浩昭
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 肝癌由来細胞株から誘導した癌幹細胞様細胞における転移能および上皮間葉系転換に関する解析 (2018)
  • Author(s): 西山光郎、恒富亮一、吉村清、松隈聰、兼清信介、飯田通久、鈴木伸明、武田茂、吉野茂文、硲彰一、上野富雄、永野浩昭
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 誘導癌幹細胞における免疫逃避機構関連遺伝子の同定 (2018)
  • Author(s): 恒富亮一、吉村清、松隈聰、西山光郎、兼清信介、徳光幸生、友近忍、飯田通久、鈴木伸明、武田茂、山本滋、吉野茂文、硲彰一、上野富雄、永野浩昭
  • Organizer: 第56回日本癌治療学会学術集会
• [Presentation] がん幹細胞様細胞と臨床サンプルとの統合解析により同定されたがん幹細胞関連遺伝子 (2018)
  • Author(s): 恒富亮一、西山光郎、藤原信行、松隈聰、兼清信介、友近忍、徳光幸生、飯田通久、鈴木伸明、武田茂、山本滋、吉野茂文、上野富雄、硲彰一、永野浩昭
  • Organizer: JDDW 2018（第26回日本消化器関連学会週間）
• [Presentation] 膵癌幹細胞に高発現した分子 calreticulin の臨床的意義 (2018)
  • Author(s): 松隈聰、吉村清、藤原康弘、井上萌子、布施雅規、小賀厚徳、恒富亮一、徳光幸生、徳久善弘、坂本和彦、兼清信介、友近忍、飯田通久、鈴木伸明、武田茂、吉野茂文、硲彰一、江口英利、上野富雄、永野浩昭
  • Organizer: JDDW 2018（第26回日本消化器関連学会週間）