• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Optimization of Rectal Cancer Treatment by Gene Mutation Patterns in Liquid biopsy and Tumor Biopsy

Research Project

Project/Area Number 18K08663
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55020:Digestive surgery-related
Research InstitutionFukuoka University

Principal Investigator

Suguru Hasegawa  福岡大学, 医学部, 教授 (10362500)

Co-Investigator(Kenkyū-buntansha) 山田 岳史  日本医科大学, 医学部, 准教授 (50307948)
吉田 陽一郎  福岡大学, 医学部, 准教授 (70580265)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords大腸癌 / 遺伝子変異 / リキッドバイオプシー / KRAS / BRAF / ctDNA / 直腸癌 / 化学放射線療法 / ccfDNA
Outline of Final Research Achievements

Liquid biopsy was performed in the perioperative period before and after surgery in patients with colorectal cancer, and the mutant allele frequencies of mutant KRAS and mutant BRAF genes in cell-free DNA were analyzed by digital PCR. The mutant allele frequencies of mutant KRAS and mutant BRAF genes in cell-free DNA were analyzed by digital PCR, and their usefulness for early prediction of cancer recurrence was examined.
The results are highly suggestive of the significance of liquid biopsy in the early recurrence of cancer, and the results suggest the need to expand the number of target genes. In the future, we will expand the number of target gene mutations and use AI-based analysis.

Academic Significance and Societal Importance of the Research Achievements

大腸癌患者の術前術後にリキッドバイオプシーを施行し、デジタルPCRによる変異型KRAS遺伝子および変異型BRAF遺伝子に関する解析をおこない、がんの再発の早期予測に対する有用性を検討した。
今回の結果のみからは再発の早期予測をすることは難しかった。さらに対象遺伝子を拡大させる必要性を感じさせる結果であった。今後は対象となる遺伝子変異を拡大させ、またAIを導入した解析を用いて検討する。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2022 2021 2020

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results)

  • [Journal Article] Relationship between perioperative oncological evaluation and recurrence using circulating tumor DNA with KRAS mutation in patients with colorectal cancer2022

    • Author(s)
      Takaomi Hayashi, Yoichiro Yoshida, Teppei Yamada, Keita Tanaka, Hideki Shimaoka, Ryuji Kajitani, Taro Munechika, Hideki Nagano, Yoshiko Matsumoto, Akira Komono, Ryohei Sakamoto, Naoya Aisu, Gumpei Yoshimatsu, Fumihiro Yoshimura, Suguru Hasegawa
    • Journal Title

      Cancer Medicine

      Volume: March21 Issue: 16 Pages: 1-10

    • DOI

      10.1002/cam4.4677

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer2021

    • Author(s)
      Keita Tanaka, Yoichiro Yoshida, Teppei Yamada, Takaomi Hayashi, Hideki Shimaoka, Fumihiro Yoshimura, Ryuji Kajitani Taro Munechika, Yoshiko Matsumoto, Hideki Nagano, Akira Komono, Ryohei Sakamoto, Ryo Nakashima, Naoya Aisu, Gumpei Yoshimatsu, Suguru Hasegawa
    • Journal Title

      Scientific Reportsorts

      Volume: 11 Issue: 1 Pages: 1-7

    • DOI

      10.1038/s41598-021-92795-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] リキッドバイオプシーによる大腸癌BRAF V600Eの周術期経時的評価2020

    • Author(s)
      田中 敬太, 吉田 陽一郎, 山田 哲平, 島岡 秀樹, 林 貴臣, 薦野 晃, 愛洲 尚哉, 吉松 軍平, 吉村 文博, 長谷川 傑
    • Organizer
      第75回日本消化器外科学会
    • Related Report
      2020 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi