Establishment of the multipurpose biomarker which targetted specific secretor exosome in esophageal cancer

• Project/Area Number: 18K08675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Mie University
• Principal Investigator: Ohi Masaki 三重大学, 医学部附属病院, 准教授 (40418752)
• Co-Investigator(Kenkyū-buntansha): 問山 裕二 三重大学, 医学系研究科, 教授 (00422824) ; 楠 正人 三重大学, 医学系研究科, 寄附講座大学教員 (50192026) ; 吉山 繁幸 三重大学, 医学系研究科, リサーチアソシエイト (60444436) ; 安田 裕美 三重大学, 医学部附属病院, 助教 (60586767) ; 荒木 俊光 三重大学, 医学系研究科, リサーチアソシエイト (70343217)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 食道癌 / パネル / 内視鏡治療 / 低侵襲手術 / 外科切除 / Exosome / 食道癌診断特異的パネル / 食道がん / エキソソーム / 蛋白 / マイクロRNA / 血清 / miRNA / エクソソーム / miRNAs

Outline of Final Research Achievements

Esophageal cancer is a disease with high medical demand because the number of mortality and morbidity do not decrease even after the development of treatment. However, after radical surgery for esophageal cancer, the QOL of patients is often impaired, such as decreased food intake, weight loss, diarrhea, and reflux symptoms. It is extremely significant from the viewpoint of medical expenses as well as contributing to the health of the patient. In this study, we identified protein X that is specifically expressed in Exosome that is secreted specifically for esophageal cancer, and clarified some clinical significance.

Academic Significance and Societal Importance of the Research Achievements

食道癌特異的に分泌されるExosomeに特異的に発現する蛋白Xを同定し、その蛋白X陽性Exosomeに包埋された遺伝子情報を解析することで、食道癌診断特異的パネルを作成し、非侵襲的なLiquid biopsyによる食道癌患者の診断から治療にわたる戦略を検証し、低侵襲的な治療の適応や予後因子などを予測できれば、内視鏡治療、低侵襲手術などの適応を早期に決定でき、国民の予後改善、治療後のQOLを改善する点でも医療費の観点からも極めて学術的意義や社会的意義は高いと思われる。

Research Products

• [Journal Article] Clinical implications of the preoperative lymphocyte C-reactive protein ratio in esophageal cancer patients (2021)
  • Author(s): Yamamoto A
  • Journal Title: Surg Today Volume: 51(5) Issue: 5 Pages: 745-755
  • DOI: 10.1007/s00595-020-02166-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Preoperative computed tomography predicts the risk of recurrent laryngeal nerve paralysis in patients with esophageal cancer undergoing thoracoscopic esophagectomy in the prone position (2021)
  • Author(s): Ohi M
  • Journal Title: Esophagus Volume: 18(2): Issue: 2 Pages: 228-238
  • DOI: 10.1007/s10388-020-00767-0
  • NAID: 40022523249
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lymphocyte-to-C-reactive protein ratio and score are clinically feasible nutrition-inflammation markers of outcome in patients with gastric cancer (2020)
  • Author(s): Okugawa Yoshinaga、Toiyama Yuji、Yamamoto Akira、Shigemori Tsunehiko、Ichikawa Takashi、Yin Chengzeng、Suzuki Akihiro、Fujikawa Hiroyuki、Yasuda Hiromi、Hiro Junichiro、Yoshiyama Shigeyuki、Ohi Masaki、Araki Toshimitsu、McMillan Donald C.、Kusunoki Masato
  • Journal Title: Clinical Nutrition Volume: 39 Issue: 4 Pages: 1209-1217
  • DOI: 10.1016/j.clnu.2019.05.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Modified neutrophil-platelet score as a promising marker for stratified surgical and oncological outcomes of patients with gastric cancer. (2020)
  • Author(s): Okugawa Y, Toiyama Y, Yamamoto A, Omura Y, Kusunoki K, Yin C, Ide S, Kitajima T, Koike Y, Fujikawa H, Yasuda H, Okita Y, Hiro J, Yoshiyama S, Ohi M, Araki T, Kusunoki M
  • Journal Title: Surg Today Volume: 50(3) Issue: 3 Pages: 223-231
  • DOI: 10.1007/s00595-019-01873-y
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance and biological role of L1 cell adhesion molecule in gastric cancer (2019)
  • Author(s): Ichikawa Takashi、Okugawa Yoshinaga、Toiyama Yuji、Tanaka Koji、Yin Chengzeng、Kitajima Takahito、Kondo Satoru、Shimura Tadanobu、Ohi Masaki、Araki Toshimitsu、Kusunoki Masato
  • Journal Title: British Journal of Cancer Volume: 121 Issue: 12 Pages: 1058-1068
  • DOI: 10.1038/s41416-019-0646-8
  • Peer Reviewed / Open Access
• [Journal Article] Laparoscopic esophagogastrostomy using a knifeless linear stapler after proximal gastrectomy. (2019)
  • Author(s): Ohi M, Toiyama Y, Kitajima T, Shigemori T, Yasuda H, Okugawa Y, Fujikawa H, Okita Y, Yokoe T, Hiro J, Araki T, Kusunoki M
  • Journal Title: Surg Today Volume: 49(12) Issue: 12 Pages: 1080-1086
  • DOI: 10.1007/s00595-019-01836-3
  • Peer Reviewed
• [Journal Article] Rac GTPase-Activating Protein 1 (RACGAP1) as an Oncogenic Enhancer in Esophageal Carcinoma (2019)
  • Author(s): Yin Chengzeng、Toiyama Yuji、Okugawa Yoshinaga、Shigemori Tsunehiko、Yamamoto Akira、Ide Shozo、Kitajima Takahito、Fujikawa Hiroyuki、Yasuda Hiromi、Okita Yoshiki、Hiro Junichiro、Yoshiyama Shigeyuki、Ohi Masaki、Araki Toshimitsu、Yao Li、Kusunoki Masato
  • Journal Title: Oncology Volume: 97 Issue: 3 Pages: 155-163
  • DOI: 10.1159/000500592
  • Peer Reviewed
• [Journal Article] Risk factors and measures of pulmonary complications after thoracoscopic esophagectomy for esophageal cancer. (2019)
  • Author(s): Ohi M, Toiyama Y, Omura Y, Ichikawa T, Yasuda H, Okugawa Y, Fujikawa H, Okita Y, Yoshiyama S, Hiro J, Araki T, Kusunoki M.
  • Journal Title: Surg Today. Volume: 49(2) Issue: 2 Pages: 176-186
  • DOI: 10.1007/s00595-018-1721-0
  • Peer Reviewed
• [Journal Article] Soluble PD-L1 Expression in Circulation as a Predictive Marker for Recurrence and Prognosis in Gastric Cancer: Direct Comparison of the Clinical Burden Between Tissue and Serum PD-L1 Expression. (2019)
  • Author(s): Shigemori T, Toiyama Y, Okugawa Y, Yamamoto A, Yin C, Narumi A, Ichikawa T, Ide S, Shimura T, Fujikawa H, Yasuda H, Hiro J, Yoshiyama S, Ohi M, Araki T, Kusunoki M.
  • Journal Title: Ann Surg Oncol. Volume: 26 Issue: 3 Pages: 876-883
  • DOI: 10.1245/s10434-018-07112-x
  • Peer Reviewed / Open Access
• [Presentation] 食道癌に対するロボット支援下食道切除術の初期成績とデバイス選択の工夫 (2020)
  • Author(s): 大井正貴、市川崇、今岡裕基、安田裕美、横江、藤川裕之、奥川喜永、大北喜基、廣純一郎、問山裕二、楠正人
  • Organizer: 第18回日本消化器外科学会大会
• [Presentation] 食道癌に対するロボット支援下食道切除術と胸腔鏡下食道切除術の短期成績の比較 (2020)
  • Author(s): 大井正貴、市川崇、今岡裕基、北嶋貴仁、安田裕美、横江毅、藤川裕之、大北喜基、廣純一郎、問山裕二、楠正人
  • Organizer: 第120回日本外科学会定期学術集会
• [Presentation] ロボット支援下食道癌手術症例におけるSSI, RI発生の検討 (2020)
  • Author(s): 大井正貴、市川崇、北嶋貴仁、今岡裕基、安田裕美、横江毅、奥川喜永、藤川裕之、大北喜、廣純一郎、問山裕二、楠正人
  • Organizer: 第33回日本外科感染症学会総会学術集会
• [Presentation] 大井正貴、市川崇、今岡裕基、北嶋貴仁、安田裕美、藤川裕之、大北喜基、廣純一郎、問山裕二、楠正人 (2020)
  • Author(s): 進行胃癌に対するロボット支援下胃切除術の検討
  • Organizer: 第75回日本消化器外科学会総会
• [Presentation] 胸腔鏡下食道癌手術症例における反回神経麻痺のリスク因子の検討 (2020)
  • Author(s): 大井正貴、市川崇、今岡裕基、北嶋貴仁、安田裕美、藤川裕之、奥川喜永、大北喜基、廣純一郎、問山裕二、楠正人
  • Organizer: 第74回日本食道学会学術集会
• [Presentation] 食道癌手術における食道胃管吻合の縫合不全の検討と吻合法の標準化 (2019)
  • Author(s): 大井正貴、市川 崇、安田裕美、吉山繁幸
  • Organizer: .第72回日本胸部外科学会定期学術集会、京都
• [Presentation] 胸部食道癌に対するロボット支援下食道切除術における手技の工夫 (2019)
  • Author(s): 大井正貴、市川 崇、今岡裕基、北嶋貴仁、安田裕美、横江 毅、藤川裕之、大北喜基、廣 純一郎、問山裕二、楠 正人
  • Organizer: 第32回日本内視鏡外科学会総会、横浜