| Project/Area Number |
18K08814
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55050:Anesthesiology-related
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | マイクロダイアリシス / TRPC / ミオグロビン / カルシウムチャネル / バイアビリティ / 心臓マイクロダイアリシス / 吸入麻酔薬 / TRPCチャネル / 心臓 / 心筋保護 |
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| Outline of Final Research Achievements |
We monitored dialysate myoglobin in the ischemic region of anesthetized rat during ischemia, using cardiac microdialysis technique. In the vehicle, dialysate myoglobin levels increased during ischemia, which were suppressed by Sevoflurane and TRPC channel blocker (2-APB, SKF96365). In hypertrophied heart rats produced by transverse aortic constriction, the dialysate myoglobin levels also increased during ischemia. Sevoflurane and TRPC channel blocker also reduced these increases in dialysate myoglobin levels during ischemia. The results suggested that TRPC channels plays a significant role in cardiac protection during ischemia. Furthermore, in two hours after ischemia, the dialysate myoglobin elevation evoked by the desipramine (cell injury-induced drug), as an index of cardiac viability, were decreased with sevoflurane. It might preserved in vivo myocardial viability against ischemia. Further study is required to define the relationship between variability and TRPC channels.
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| Academic Significance and Societal Importance of the Research Achievements |
吸入麻酔薬が心筋虚血による細胞傷害の抑制効果を有すること、その機序としてTRPCチャネル（カルシウムチャネルの一種）が関与ししていることをラット心臓において検証した。この結果は全身麻酔時に使用する吸入麻酔薬が狭心症などの虚血性疾患を有する患者においてより安全な麻酔医療を提供できる可能性を示唆する。さらに、その機序を詳細に探索することで心保護効果をさらに増強する麻酔法が開発できる。
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