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Effects of TRPC channel suppressed by inhaled anesthetics on myocardial protection

Research Project

Project/Area Number 18K08814
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55050:Anesthesiology-related
Research InstitutionShiga University of Medical Science

Principal Investigator

Hirotoshi Kitagawa  滋賀医科大学, 医学部, 教授 (50252391)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsマイクロダイアリシス / TRPC / ミオグロビン / カルシウムチャネル / バイアビリティ / 心臓マイクロダイアリシス / 吸入麻酔薬 / TRPCチャネル / 心臓 / 心筋保護
Outline of Final Research Achievements

We monitored dialysate myoglobin in the ischemic region of anesthetized rat during ischemia, using cardiac microdialysis technique. In the vehicle, dialysate myoglobin levels increased during ischemia, which were suppressed by Sevoflurane and TRPC channel blocker (2-APB, SKF96365). In hypertrophied heart rats produced by transverse aortic constriction, the dialysate myoglobin levels also increased during ischemia. Sevoflurane and TRPC channel blocker also reduced these increases in dialysate myoglobin levels during ischemia. The results suggested that TRPC channels plays a significant role in cardiac protection during ischemia. Furthermore, in two hours after ischemia, the dialysate myoglobin elevation evoked by the desipramine (cell injury-induced drug), as an index of cardiac viability, were decreased with sevoflurane. It might preserved in vivo myocardial viability against ischemia. Further study is required to define the relationship between variability and TRPC channels.

Academic Significance and Societal Importance of the Research Achievements

吸入麻酔薬が心筋虚血による細胞傷害の抑制効果を有すること、その機序としてTRPCチャネル(カルシウムチャネルの一種)が関与ししていることをラット心臓において検証した。この結果は全身麻酔時に使用する吸入麻酔薬が狭心症などの虚血性疾患を有する患者においてより安全な麻酔医療を提供できる可能性を示唆する。さらに、その機序を詳細に探索することで心保護効果をさらに増強する麻酔法が開発できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (2 results)

All 2020

All Presentation (2 results)

  • [Presentation] セボフルランのIKsチャネル抑制作用はβサブユニットをコードするKCNE1遺伝子の一塩基多型D85Nにより増強し、セボフルランによるQT延長作用を増強する2020

    • Author(s)
      小嶋亜希子、伊藤有紀、福島豊、今宿康彦、北川裕利
    • Organizer
      日本麻酔科学会第67回学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] デスフルランはヒト心筋の膜電位依存性カリウムチャネルに対して異なった抑制作用を発揮する2020

    • Author(s)
      福島豊、小嶋亜希子、石原真理子、清水盛浩、湯浅真由美、北川裕利
    • Organizer
      日本麻酔科学会第67回学術集会
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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