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Investigation of the effects of anesthetics on cancer tissue phenotype and gene expression using glioma stem cells

Research Project

Project/Area Number 18K08874
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55050:Anesthesiology-related
Research InstitutionKansai Medical University

Principal Investigator

IWAI Teppei  関西医科大学, 医学部, 研究員 (90440966)

Co-Investigator(Kenkyū-buntansha) 広田 喜一  関西医科大学, 医学部, 教授 (00283606)
岩田 亮一  関西医科大学, 医学部, 非常勤講師 (60580446)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords麻酔薬 / 神経膠腫 / がん幹細胞 / 三次元スフェア培養 / RNA-Seq法 / がん
Outline of Final Research Achievements

Glioma-derived cancer stem cells were treated with anesthetics under ex vivo (in vitro spheroid culture) and in vitro (in vitro monoculture) conditions to examine the effects of anesthetics on the phenotype of glioma-derived cancer stem cells (proliferative capacity, metabolic mode, and cell death) and the effects of anesthetics on the genetic system of glioma-derived cancer stem cells (gene expression). 6-hour exposure of MD13 and Me83 cancer stem cell-like cells to sevoflurane, a volatile inhalant anesthetic at 2% clinical concentration, suppresses sphere formation without affecting proliferation or cell death.

Academic Significance and Societal Importance of the Research Achievements

固形腫瘍としての癌は単なる単細胞の集合体ではなく、単細胞樹立細胞株を用いた麻酔薬の影響についての従来の研究は多くのバイアス故に臨床的な実態とはかけ離れている。患者の予後への影響を見るためには、がん組織特有の表現型を解析対象とすべきである。麻酔薬のがんに対する影響の検討は樹立細胞株を用いた単細胞培養細胞について主に行われてきたがグリオーマ幹細胞を用いた検討は報告がない。この点で本研究はユニークな視点からのエビデンスを提供する事になる。
この治験はがん治療の帰趨に関わる重要な要素であり社会的な意義も大きいと考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

Research Products

(11 results)

All 2020 2019 2018

All Journal Article (10 results) (of which Peer Reviewed: 8 results,  Open Access: 7 results) Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition.2020

    • Author(s)
      Boku S, Watanabe M, Sukeno M, Yaoi T, Hirota K, Iizuka-Ohashi M, Itoh K, Sakai T.
    • Journal Title

      Cancers

      Volume: 12 Pages: 1097-1097

    • DOI

      10.3390/cancers12051097

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meta-Analysis of Hypoxic Transcriptomes from Public Databases.2020

    • Author(s)
      Bono H, Hirota K
    • Journal Title

      Biomedicines

      Volume: 8 Pages: 1-1

    • DOI

      10.3390/biomedicines8010010

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Basic Biology of Hypoxic Responses Mediated by the Transcription Factor HIFs and its Implication for Medicine2020

    • Author(s)
      Hirota K.
    • Journal Title

      Biomedicines

      Volume: 8 Pages: 2-2

    • DOI

      10.3390/biomedicines8020032

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ミトコンドリアの酸素代謝異常と疾患2020

    • Author(s)
      広田 喜一
    • Journal Title

      医学のあゆみ

      Volume: 274 Pages: 248-252

    • Related Report
      2020 Annual Research Report
  • [Journal Article] An intimate crosstalk between iron homeostasis and oxygen metabolism regulated by the hypoxia-inducible factors (HIFs)2019

    • Author(s)
      Hirota K
    • Journal Title

      Free Radic Biol Med

      Volume: 133 Pages: 118-29

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 酸素生物学の建設 ― 生体の酸素利用の方程式 ―2019

    • Author(s)
      広田 喜一
    • Journal Title

      現代化学

      Volume: 585 Pages: 29-32

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 29-322019

    • Author(s)
      広田 喜一
    • Journal Title

      実験医学

      Volume: 37 Pages: 3249-50

    • Related Report
      2019 Research-status Report
  • [Journal Article] Cancerous phenotypes associated with hypoxia-inducible factors are not influenced by the volatile anesthetic isoflurane in renal cell carcinoma2019

    • Author(s)
      Sumi Chisato、Matsuo Yoshiyuki、Kusunoki Munenori、Shoji Tomohiro、Uba Takeo、Iwai Teppei、Bono Hidemasa、Hirota Kiichi
    • Journal Title

      PLoS ONE

      Volume: 14

    • DOI

      10.1371/journal.pone.0215072

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An intimate crosstalk between iron homeostasis and oxygen metabolism regulated by the hypoxia-inducible factors (HIFs)2019

    • Author(s)
      Hirota Kiichi
    • Journal Title

      Free Radical Biology and Medicine

      Volume: 133 Pages: 118-129

    • DOI

      10.1016/j.freeradbiomed.2018.07.018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suppression of mitochondrial oxygen metabolism mediated by the transcription factor HIF-1 alleviates propofol-induced cell toxicity2018

    • Author(s)
      Sumi Chisato、Okamoto Akihisa、Tanaka Hiromasa、Kusunoki Munenori、Shoji Tomohiro、Uba Takeo、Adachi Takehiko、Iwai Teppei、Nishi Kenichiro、Harada Hiroshi、Bono Hidemasa、Matsuo Yoshiyuki、Hirota Kiichi
    • Journal Title

      Sci Rep

      Volume: 8 Pages: 8987-8987

    • DOI

      10.1038/s41598-018-27220-8

    • NAID

      120006539570

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 低酸素-低酸素誘導性因子(HIF)-炎症2019

    • Author(s)
      広田 喜一
    • Organizer
      第72回日本酸化ストレス学会学術集会
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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