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Evaluation of abnormal tract using two-photon excited fluorescence microscopy in SBA

Research Project

Project/Area Number 18K08945
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56010:Neurosurgery-related
Research InstitutionEhime University

Principal Investigator

Matsuda seiji  愛媛大学, 医学系研究科, 教授 (40173843)

Co-Investigator(Kenkyū-buntansha) カーン モハメドシャキル  愛媛大学, 医学系研究科, 助教(特定教員) (70746867)
鍋加 浩明  愛媛大学, 医学系研究科, 准教授 (60581098)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords二分脊椎 / ニワトリ / 歩行障害 / 脊髄 / 神経回路 / 運動神経 / 感覚神経 / 出生前治療 / 鶏 / 二光子顕微鏡 / プロサポシン / 神経栄養因子 / 二分脊髄 / 立体画像 / ヒヨコ / 脊髄回路
Outline of Final Research Achievements

Applicants reopened the spinal cord by surgery, created the spina bifida chick model, and reported about small nerve depletion, delayed motor nerve development, and abnormal sensory nerve running. We summarized the neuropathy in this model, discovered that motor function deteriorated sharply after childbirth, and published a paper. In addition, we have succeeded in observing the sensory nerve fibers of the chicken spinal cord with a two-photon microscope expert. Currently, using a two-photon microscope, the abnormal neural circuit of spina bifida is analyzed three-dimensionally, and a paper about the neurological pathology of this disease is submitted. Since the success of this method has facilitated stereoscopic observation, the therapeutic effect of spina bifida with a synthetic peptide of prosaposin is also being observed using a two-photon microscope and data is being accumulated.

Academic Significance and Societal Importance of the Research Achievements

二分脊椎症は運動、排尿障害、下肢の変形等が起こる整形外科、脳神経外科、小児科等の領域で重要な疾患である。申請者らは、手術により脊髄を再開裂し、二分脊椎ヒヨコモデルを世界で初めて作成することに成功した(Mominoki ら2006)。二分脊椎における脊髄内神経回路障害に関しては、解析が充分では無い。特に、ヒトにおいて、出産時にある程度残った運動機能が、出産後に急激に悪化することが臨床上重要である。申請者は本モデルでも出産後に運動機能が急激に悪化することを発見し、論文発表した。本研究結果から、脊髄内神経回路障害に関して解析が進み、二分脊椎の治療を行うことが可能となれば、社会的意義は極めて大きい。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (29 results)

All 2021 2020 2019 2018

All Journal Article (16 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 16 results,  Open Access: 15 results) Presentation (13 results)

  • [Journal Article] Expression of the G protein-coupled receptors GPR37 and GPR37L1 in the mouse digestive system.2021

    • Author(s)
      Sarkar S, Homma T, Onouchi S, Shimizu Y, Shiina T, Nabeka H, Matsuda S, Saito S.
    • Journal Title

      J Veter Med Sci

      Volume: in press

    • NAID

      130007967844

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 16) Postmortem interval estimation by evaluating saposin D levels and morphological alterations in hippocampal neurons.2021

    • Author(s)
      Nakabayashi Y, Nabeka H, Kuwahara N, Matsuda S, Asano M.
    • Journal Title

      Albanian J Med Health Sci.

      Volume: in press

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prosaposin and its Receptors, GRP37 and GPR37L1, Protects Neurons Against In Vivo Neuropathological Disorders2021

    • Author(s)
      NabekaH, Gao HL, Li X, Li C, Wakisaka H, Kunihiro J, Unuma K, Taniguchi M, Nakabayashi Y, Khan MSI, Shimokawa T, Islam F, Saito S, Hamada F, Kobayashi N, Matsuda S.
    • Journal Title

      Online Journal of Neurology and Brain Disorders.

      Volume: in press

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Suppression of GABAergic transmission in the spinal dorsal horn induces pain-related behaviour in a?chicken model of spina bifida2020

    • Author(s)
      Khan Md. Sarikul、Nabeka Hiroaki、Islam Farzana、Shimokawa Tetsuya、Saito Shouichiro、Tachibana Tetsuya、Matsuda Seiji
    • Journal Title

      Folia Neuropathologica

      Volume: 58 Issue: 2 Pages: 151-165

    • DOI

      10.5114/fn.2020.96800

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Age- and sex-associated changes in prosaposin and its receptors in the lacrimal glands of rats.2020

    • Author(s)
      Islam F, Khan MSI(CA), Nabeka H, Saito S, Shimokawa T, Yamamiya K, Matsuda S.
    • Journal Title

      Histol Histopathol

      Volume: 35 Pages: 69-81

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Risk of congenital birth defects during COVID-19 pandemic: Draw attention to the physicians and policymakers2020

    • Author(s)
      Khan Md Sakirul Islam、Nabeka Hiroaki、Akbar Sheikh Mohammad Fazle、Al Mahtab Mamun、Shimokawa Tetsuya、Islam Farzana、Matsuda Seiji
    • Journal Title

      Journal of Global Health

      Volume: 10 Issue: 2 Pages: 1-4

    • DOI

      10.7189/jogh.10.020378

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ivermectin Treatment May Improve the Prognosis of Patients With COVID-192020

    • Author(s)
      Khan Md. Saiful Islam、Khan Md. Sakirul Islam、Debnath Chitto Ranjan、Nath Progga Nanda、Mahtab Mamun Al、Nabeka Hiroaki、Matsuda Seiji、Akbar Sheikh Mohammad Fazle
    • Journal Title

      Archivos de Bronconeumolog?a

      Volume: 56 Issue: 12 Pages: 828-830

    • DOI

      10.1016/j.arbres.2020.08.007

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prosaposin in the rat oviductal epithelial cells2020

    • Author(s)
      Shimokawa Tetsuya、Nabeka Hiroaki、Khan Sakirul Islam、Yamamiya Kimiko、Doihara Takuya、Kobayashi Naoto、Wakisaka Hiroyuki、Matsuda Seiji
    • Journal Title

      Cell and Tissue Research

      Volume: 383 Issue: 3 Pages: 1191-1202

    • DOI

      10.1007/s00441-020-03339-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prosaposin and its receptors GRP37 and GPR37L1 show increased immunoreactivity in the facial nucleus following facial nerve transection2020

    • Author(s)
      Kunihiro Joji、Nabeka Hiroaki、Wakisaka Hiroyuki、Unuma Kana、Khan Md. Sakirul Islam、Shimokawa Tetsuya、Islam Farzana、Doihara Takuya、Yamamiya Kimiko、Saito Shouichiro、Hamada Fumihiko、Matsuda Seiji
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 12 Pages: 0241315-0241315

    • DOI

      10.1371/journal.pone.0241315

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Age- and sex-associated changes in prosaposin and its receptors in the lacrimal glands of rats2020

    • Author(s)
      Farzana Islam, Sakirul Islam Khan,Hiroaki Nabeka, Tetsuya Shimokawa, Kimiko Yamamiya and Seiji Matsuda
    • Journal Title

      Hisotology and Histopathology

      Volume: 35 Pages: 69-81

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Revisiting the infracardiac bursa by multimodal methods.2019

    • Author(s)
      Nakamura T, Shinohara H, Okada T, Hisamori S, Tsunoda S, Obama K, Kurahashi Y, Takai A, Shimokawa T, Matsuda S, Makishima H, Takakuwa T, Yamada S, Sakai Y.
    • Journal Title

      J Anat,

      Volume: 印刷中 Issue: 1 Pages: 88-95

    • DOI

      10.1111/joa.12989

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Precise three-dimensional morphology of the male anterior anorectum reconstructed from large serial histological sections: a cadaveric study2019

    • Author(s)
      Tomoaki Okada, Suguru Hasegawa, Tatsuro Nakamura, Koya Hida, Kenji Kawada, Akihiro Takai, M, Tetsuya Shimokawa, Seiji Matsuda, Hisashi Shinohara, Yoshiharu Sakai
    • Journal Title

      Diseases of the Colon & Rectum

      Volume: 62(10) Issue: 10 Pages: 1238-1247

    • DOI

      10.1097/dcr.0000000000001449

    • NAID

      120006898062

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Compound 48/80 reduces the crop-emptying rate, likely through a histamine-associated pathway in chicks.2019

    • Author(s)
      Tachibana T, Ueoka W, Khan MSI, Makino R, Cline MA.
    • Journal Title

      Domestic Animal Endocrinology

      Volume: 66 Pages: 57-63

    • DOI

      10.1016/j.domaniend.2018.10.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Physiological responses to central and peripheral injection of polyinosinic-polycytidylic acid in chicks.2019

    • Author(s)
      Tachibana T, Ishimaru Y, Takeda K, Khan MSI, Makino R, Cline MA.
    • Journal Title

      British Poultry Science

      Volume: 60 Issue: 1 Pages: 64-70

    • DOI

      10.1080/00071668.2018.1547361

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prosaposin and its receptors are differentially expressed in the salivary glands of male and female rats.2018

    • Author(s)
      Islam F, Khan MSI, Nabeka H, Saito S, Li X, Shimokawa T, Yamamiya K, Kobayashi N, Matsuda S.
    • Journal Title

      Cell and Tissue Research

      Volume: 373 Issue: 2 Pages: 439-457

    • DOI

      10.1007/s00441-018-2835-9

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The tumor suppressor menin prevents effector CD8 T cell dysfunction by targeting mTORC1-dependent metabolic activation2018

    • Author(s)
      Junpei Suzuki, Takeshi Yamada, Kazuki Inoue, Shogo Nabe, Makoto Kuwahara, Nobuaki Takemori, Ayako Takemori, Seiji Matsuda, Makoto Kanoh, Yuki Imai, Masaki Yasukawa, and Masakatsu Yamashita
    • Journal Title

      Nature Communications

      Volume: 9

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Prosaposin derived 18-mer peptide enables in ovo regeneration of neuroal tube defects.2020

    • Author(s)
      Md. Sakirul Islam Khan, Hiroaki Nabeka, Farzana Islam, Tetsuya Shimokawa, Kimiko Yamamiya, Takuya Doihara,Seiji Matsuda
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Systemic injection of neurotoxin alters prosaposin level in the salivary system of rats2020

    • Author(s)
      Islam F, Khan MSI, Nabeka H, Shimokawa T, Yamamiya K, Doihara T, Matsuda S
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 手術的二分脊椎モデルにおける脊髄運動神経細胞及び介在神経細胞の変化2020

    • Author(s)
      中村優花、Khan MSI、鍋加浩明、下川哲哉、土居原拓也、山宮公子、松田正司
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 精子形成におけるプロサポシンの役割2020

    • Author(s)
      山宮公子、鍋加浩明、下川哲哉、Khan MSI、小林直人、松田正司
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脳、胸部臓器の死後変化に関する研究2020

    • Author(s)
      灘野愛唯、鍋加浩明、中林ゆき、Khan MSI、下川哲哉、土居原拓也、山宮公子、松田正司
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Prosaposin derived 18-mer peptide enables in ovo regeneration of neuroal tube defects.2020

    • Author(s)
      Md. Sakirul Islam Khan, Hiroaki Nabeka, Farzana Islam, Tetsuya Shimokawa, Kimiko Yamamiya, Takuya Doihara,Seiji Matsuda
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Prosaposin derived 18-mer peptide promotes neural tube regeneration in a chicken model of spina bifida aperta2019

    • Author(s)
      Md. Sakirul Islam Khan, Hiroaki Nabeka, Farzana Islam, Tetsuya Shimokawa, Takuya Doihara, Kimiko Yamamiya, Seiji Matsuda
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Prosaposin derived 18-mer peptide promotes neural tube regeneration in a chicken model of spina bifida aperta.2019

    • Author(s)
      Khan MSI, Nabeka H, Islam F, Shimokawa T, Yamamiya K, Doihara T, Matsuda S.
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Age- and sex-related alteration of prosaposin and its receptors in the lacrimal glands of rats.2019

    • Author(s)
      Islam F, Khan MSI, Nabeka H, Shimokawa T, Yamamiya K, Doihara T, Matsuda S.
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] ラット卵管におけるプロサポシンとプロサポシン受容体の分布2019

    • Author(s)
      下川哲哉、鍋加浩明、Khan MSI、土居原拓也、脇坂浩之、小林直人、松田正司
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 培養細胞における神経栄養因子プロサポシン細胞内動態2019

    • Author(s)
      鍋加浩明、土居原拓也、Khan MSI、下川哲哉、小林直人、川合克久、荒木伸一、松田正司
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Function of prosaposin in the testis.2019

    • Author(s)
      Yamamiya K, Li X, Nabeka H, Shimokawa T, Khan MSI, Doihara T, Saito S, Hamada F, Matsuda S, Kobayashi
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 発生期の後根神経節におけるプロサポシンとその受容体の変化2019

    • Author(s)
      谷口実帆、山宮公子、鍋加浩明、Khan MSI, 下川哲哉、土居原拓也、小林直人、松田正司
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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