• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of neuronal tumorigenesis using mouse models

Research Project

Project/Area Number 18K09013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56010:Neurosurgery-related
Research InstitutionChiba Cancer Center (Research Institute)

Principal Investigator

Isogai Eriko  千葉県がんセンター(研究所), がんゲノムセンター 実験動物研究部, 上席研究員 (40300917)

Co-Investigator(Kenkyū-buntansha) 若林 雄一  千葉県がんセンター(研究所), がんゲノムセンター 実験動物研究部, 部長 (40303119)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsマウスモデル / 神経系腫瘍 / 神経発生
Outline of Final Research Achievements

Meis1 that is involved in maintenance of stem cells and tumorigenesis, is amplified in neuronal tumors. In Meis1 knockout mice (KO), abnormal embryonic brain was observed and proliferation of radial glial cells was decreased. When Meis1 was knocked out in neural stem cells of embryonic brain, migration of neural stem cells was impaired during cortical development. Intracranial injection of neural stem cell line overexpressing Meis1 promoted brain tumor malignancy. To search genes that play important roles in the malignant brain tumor, RNA-seq was done using RNA prepared from the tumors developed after intracranial transplantation of cells. Genes that are involved in glioblastoma and neuronal differentiation were identified. These results suggest that Meis1 could play roles in developmental process of brain and brain tumor malignancy and hence the analysis may help elucidate the mechanism of brain tumor development.

Academic Significance and Societal Importance of the Research Achievements

幹細胞の維持と腫瘍形成に関与するMeis1は中枢および末梢神経系の発生に重要な働きをするとともに水頭症の発症に関与している。本研究ではさらにMeis1が大脳発生や脳腫瘍の発生及び悪性化に関与することが示唆された。従って、Meis1やその下流の遺伝子が関わるネットワークを明らかにすることで、発症機構が未だ不明な神経芽種、脳腫瘍や先天性水頭症など神経系腫瘍や神経疾患の解明に発展することが期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (16 results)

All 2021 2020 2019 2018 Other

All Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] The Japanese Wild-Derived Inbred Mouse Strain, MSM/Ms in Cancer Research.2021

    • Author(s)
      Kazuhiro Okumura, Megumi Saito, Eriko Isogai, Yuichi Wakabayashi
    • Journal Title

      Cancers

      Volume: 13 Issue: 5 Pages: 1026-1039

    • DOI

      10.3390/cancers13051026

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model.2020

    • Author(s)
      2.Saito M, Kagawa N, Okumura K, Munakata H, Isogai E, Fukagawa T, Wakabayashi Y.
    • Journal Title

      Cancer Science.

      Volume: 111 Issue: 8 Pages: 2850-2860

    • DOI

      10.1111/cas.14533

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pak1 maintains epidermal stem cells by regulating Langerhans cells and is required for skin carcinogenesis2020

    • Author(s)
      Okumura K,Saito M, Yoshizawa Y, Ito Y, Isogai E, Araki K, Wakabayashi Y
    • Journal Title

      Oncogene

      Volume: - Issue: 24 Pages: 4756-4769

    • DOI

      10.1038/s41388-020-1323-3

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Polymorphic Variant in p19Arf Confers Resistance to Chemically Induced Skin Tumors by Activating the p53 Pathway2019

    • Author(s)
      Saito M, Okumura K, Isogai E, Araki K, Tanikawa C, Matsuda K, Kamijo T, Kominami R, Wakabayashi Y.
    • Journal Title

      Journal of Investigative Dermatology

      Volume: in press Issue: 7 Pages: 30032-6

    • DOI

      10.1016/j.jid.2018.12.027

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マウス系統間のPak1-3`UTRのSNPsは発現減少と腫瘍抑制に関連する2019

    • Author(s)
      奥村和弘、斎藤慈、磯貝恵理子、荒木 喜美、若林雄一
    • Organizer
      第66回日本実験動物学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 順遺伝学に基づくMSMマウスの発がん抵抗性の解明2019

    • Author(s)
      奥村和弘、斎藤慈、磯貝恵理子、荒木 喜美、若林雄一
    • Organizer
      第34回発癌病理研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 日本産野生由来近交系マウスMSMを用いた皮膚がん修飾因子の同定2019

    • Author(s)
      奥村和弘、斎藤慈、磯貝恵理子、若林雄一
    • Organizer
      第78回日本癌学会学術総会
    • Related Report
      2019 Research-status Report
  • [Presentation] Meis1の大脳皮質発生への関連性の検討2019

    • Author(s)
      磯貝恵理子、奥村和弘、斎藤慈、吉澤康博、田村優希、若林雄一
    • Organizer
      第32回モロシヌス研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] The genetic polymorphism in p19Arf confers resistance to tumor progression.2018

    • Author(s)
      Megumi Saito, Kazuhiko Okumura, Eriko Isogai, Kimi Araki, Yuichi Wakabayashi
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Identification of responsible genes for Stmm1a locus conferring resistance to early-stage chemically induced skin tumors.2018

    • Author(s)
      Kazuhiko Okumura, Megumi Saito, Eriko Isogai, Kimi Araki, Yuichi Wakabayashi
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん抑制遺伝子p19Arfに存在する非同義置換多型の腫瘍悪性化における役割2018

    • Author(s)
      齋藤慈、奧村和弘、磯貝恵理子、荒木喜美、若林雄一
    • Organizer
      第31回モロシヌス研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] 副甲状腺ホルモンPTHの腫瘍増殖抑制効果2018

    • Author(s)
      奧村和弘、齋藤慈、磯貝恵理子、若林雄一
    • Organizer
      第27回癌病態治療研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん抑制遺伝子p19Arfに存在する非同義置換多型の機能解析2018

    • Author(s)
      齋藤慈、奧村和弘、磯貝恵理子、若林雄一
    • Organizer
      第27回癌病態治療研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] 順遺伝学的手法により明らかとなったマウス副甲状腺ホルモンの腫瘍抑制効果2018

    • Author(s)
      奧村和弘、齋藤慈、磯貝恵理子、三浦郁生、若菜茂晴、山口碧、設楽浩志、多屋長治、木南凌、若林雄一
    • Organizer
      第65回日本実験動物学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] がん抑制遺伝子p19Arfに存在する非同義置換多型の機能解析2018

    • Author(s)
      齋藤慈、奧村和弘、磯貝恵理子、若林雄一
    • Organizer
      第65回日本実験動物学会総会
    • Related Report
      2018 Research-status Report
  • [Remarks]

    • URL

      http://www.pref.chiba.lg.jp/gan/kenkyujo/index.html

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi