Establishment of rapidly identification of unknown pathogenic microorganisms using melting temperature mapping method in bone joint soft tissue infections
| Project/Area Number |
18K09043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56020:Orthopedics-related
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 人工関節周囲感染 / 感染 / PCR / Tm mapping 法 / 16S-rRNA / 関節液 / 骨関節軟部組織感染症 / 菌数定量的検査 / Tm mapping / Tm mapping法 / PCR法 / 原因菌種迅速同定法 / シーケンス解析 |
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| Outline of Final Research Achievements |
The melting temperature mapping method is a new rapid identification system for the causative agent of septicemia developed by Dr. Niimi et al.
In this study, the melting temperature mapping method was used for rapid diagnosis of bone joint soft tissue infection. In order to examine the reliability of tests using infected soft tissue other than joint fluid and to establish a diagnostic method for multiple bacterial infections, we confirmed multiple unknown pathogenic microorganisms using a next-generation sequencer.
We also evaluated the usefulness of the quantitative test of "bacterial count" in the diagnosis of periprosthetic joint infection, and examined the cut-off value of the bacterial count.
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| Academic Significance and Societal Importance of the Research Achievements |
Tm mapping 法ではプライマー数を７つに固定して広範な菌種を同定できる。データベースの修正・拡張が容易であり、新たなmutant strainが出現しても直ぐに登録して、同定に反映させることが出来る。塩基配列を読むことなく、PCRとmelting解析のみで同定できるため、迅速・簡便・安価な同定が可能であり、耐性菌の診断や菌数定量が可能であることから治療効果の判定に応用できる可能性がある。
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Report
(5 results)
Research Products
(31 results)
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[Presentation] 人工関節周囲感染の診断－バイオマーカーとPCR－2019
Author(s)
内山勝文,櫻井慶造,仁井見英樹,神田宏美,峯岸洋次郎,池田信介,福島健介,森谷光俊,狩野有作,高平尚伸,髙相晶士
Organizer
第34回日本整形外科学会基礎学術集会
Related Report
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[Presentation] 人工関節周囲感染における迅速診断の試み－PCR法とα-Defensin－2019
Author(s)
内山勝文,櫻井慶造,仁井見英樹,神田宏美,峯岸洋次郎,池田信介,福島健介,高平尚伸,高相晶士,高山陽子,和田達彦,中村正樹,花木秀明髙相晶士
Organizer
第93回日本感染症学会総会・学術講演会
Related Report
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