Analysis of the role of miR-34a in osteosarcoma stem cell models and its potential therapeutic targets
| Project/Area Number |
18K09058
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56020:Orthopedics-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山本 靖彦 金沢大学, 医学系, 教授 (20313637)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨肉腫 / がん幹細胞 / 糖化蛋白受容体 / miR-34a / Wnt / c-MYC / 骨・軟部腫瘍 / miRNA |
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| Outline of Final Research Achievements |
RAGE (receptor for advanced glycation end-products) overexpressing cells in osteosarcoma (HOS-RAGE)) expressed multiple stem cell markers compared to control (HOS-mock) cells. Moreover, HOS-RAGE cells showed the low expression of miR-34a and increased expression of c-MYC and Wnt-5a. Furthermore, the addition of synthetic miR34a decreased the ability to tumorigenicity (spheroid formation), reduced expression of c-MYC and Wnt-5a, and increased expression of PD-L1. HOS-RAGE might have the drug resistance and tumorigenicity of cancer stem cells themselves and the ability to escape the immune system
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| Academic Significance and Societal Importance of the Research Achievements |
後期糖化産物受容体(Receptor for advanced glycation end-products: RAGE)により骨肉腫細胞が幹細胞様の性質を獲得することを見出し、そのメカニズムとして、miR34aの低下とc-Myc、Wnt5aの亢進が関与していることと、がんの微小環境に関わるとされるPD-L1が高発現していることを見出した。近年、がんの微小環境のメカニズムや治療標的に関する研究が進んでおり、miR34aやWnt5aが重要な役割を担っていることも報告されつつあり本研究がmiR34aやWnt5aを介する微小環境と腫瘍とのクロストークや治療標的に関する研究に発展する可能性が示された。
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Report
(4 results)
Research Products
(74 results)
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[Journal Article] Surgical outcomes of patients with diffuse-type tenosynovial giant-cell tumours: an international, retrospective, cohort study2019
Author(s)
Mastboom MJL, Staals EL, Verspoor FGM, Rueten-Budde AJ, Stacchiotti S, Palmerini E, Schaap GR, Jutte PC, Aston W, Leithner A, Dammerer D, Takeuchi A, Thio Q, Niu X, Wunder JS, van de Sande MAJ; Tenosynovial Giant Cell Tumors (TGCT) Study Group.
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Journal Title
The Lancet Oncology
Volume: 20
Issue: 6
Pages: 877-886
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Anti-tumor effects of a nonsteroidal anti-inflammatory drug zaltoprofen on chondrosarcoma via activating peroxisome proliferator-activated receptor gamma and suppressing matrix metalloproteinase-2 expression.2018
Author(s)
Higuchi T, Takeuchi A, Munesue S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Inatani H, Shimozaki S, Kato T, Aoki Y, Abe K, Taniguchi Y, Aiba H, Murakami H, Harashima A, Yamamoto Y, Tsuchiya H.
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Journal Title
Cancer Med.
Volume: 印刷中
Issue: 5
Pages: 1944-1954
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Novel treatment of Zaltoprofen for diffuse-type tenosynovial giant cell tumor arising in knee and ankle joint: a pilot study2019
Author(s)
Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Tsuchiya H
Organizer
32th Annual Meeting of the European Musculo Skeletal Oncology Society (EMSOS)
Related Report
Int'l Joint Research
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[Presentation] Residual epiphysis growth after epiphyseal-preservation surgery for childhood osteosarcoma around the knee joint.2019
Author(s)
Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Tsuchiya H
Organizer
32th Annual Meeting of the European Musculo Skeletal Oncology Society (EMSOS)
Related Report
Int'l Joint Research
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[Presentation] Ultrasonography-guided surgery for impalpable and ill-defined malignant soft tissue tumor2019
Author(s)
Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Tsuchiya H
Organizer
32th Annual Meeting of the European Musculo Skeletal Oncology Society (EMSOS)
Related Report
Int'l Joint Research
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[Presentation] Mid- to long-term clinical outcome of tumor-devitalized frozen autografts for segmental defect of long bones after tumor excision2019
Author(s)
Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Tsuchiya H
Organizer
20th International Society of Limb Salvage General Meeting （ISOLS）
Related Report
Int'l Joint Research
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[Presentation] Intraoperative ultrasonography-guided surgery for impalpable and ill-defined malignant soft tissue tumor2019
Author(s)
Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Tsuchiya H
Organizer
20th International Society of Limb Salvage General Meeting （ISOLS）
Related Report
Int'l Joint Research
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[Presentation] Factor analysis associated with local recurrence and limb function of 105 patients with diffuse-type tenosynovial giant cell tumor: A multinational study of an Asian population2019
Author(s)
Takeuchi A, Niu X, Chen WM, Nakamura T, Nathan SS, Ueda T, Iwata S, Kawai A, Kang YK, Asavamongkolkul A, Wang EHM, Singh VA, Shirai T, Tsuchiya H
Organizer
20th International Society of Limb Salvage General Meeting （ISOLS）
Related Report
Int'l Joint Research
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[Presentation] Novel treatment of Zaltoprofen for diffuse-type tenosynovial giant cell tumor: a pilot study2019
Author(s)
Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Tsuchiya H
Organizer
Annual Meeting of the Connective Tissue Oncology Society (CTOS)
Related Report
Int'l Joint Research
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[Presentation] Factor analysis associated with local recurrence and limb function of 105 patients with diffuse-type tenosynovial giant cell tumor: A multinational study of an Asian population2019
Author(s)
Takeuchi A, Niu X, Chen WM, Nakamura T, Nathan SS, Ueda T, Iwata S, Kawai A, Kang YK, Asavamongkolkul A, Wang EHM, Singh VA, Shirai T, Tsuchiya H
Organizer
Annual Meeting of the Connective Tissue Oncology Society (CTOS)
Related Report
Int'l Joint Research
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[Presentation] Long-term clinical outcomes of 105 patients with diffuse-type tenosynovial giant cell tumor: The East Asia Musculo-skeletal Oncology Group (EAMOG) study2018
Author(s)
Takeuchi A, Niu X, Chen WM, Nakamura T, Nathan SS, Ueda T, Iwata S, Kawai A, Kang YK, Asavamongkolkul A, Wang EHM, Singh VA, Shirai T, Tsuchiya H
Organizer
第91回日本整形外科学会学術集会
Related Report
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[Presentation] びまん型腱滑膜巨細胞腫の長期成績：東アジア骨軟部腫瘍研究グループ多施設共同研究2018
Author(s)
武内章彦, 山本憲男, Niu X, Chen WM, 中村知樹, Nathan SS, 上田孝文, 岩田慎太郎, 川井 章, Kang YK, Asavamongkolkul A, 白井寿治, 土屋弘行
Organizer
第51回日本整形外科学会 骨・軟部腫瘍学術集会
Related Report
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[Presentation] Long-term clinical outcomes of 105 patients with diffuse-type tenosynovial giant cell tumor: The East Asia Musculo-skeletal Oncology Group (EAMOG) study2018
Author(s)
Takeuchi A, Niu X, Chen WM, Nakamura T, Nathan SS, Ueda T, Iwata S, Kawai A, Kang YK, Asavamongkolkul A, Wang EHM, Singh VA, Shirai T, Tsuchiya H
Organizer
12th Asia Pacific Musculoskeletal Tumor Society（APMSTS）meeting
Related Report
Int'l Joint Research
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