Elucidation of the pharmacological action of the freezed-dried platlet-rich plasma and development of new bone -forming material
| Project/Area Number |
18K09093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56020:Orthopedics-related
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| Research Institution | Chiba University |
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Principal Investigator |
MASAHIKO SUZUKI 千葉大学, フロンティア医工学センター, 教授 (10312951)
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| Co-Investigator(Kenkyū-buntansha) |
大鳥 精司 千葉大学, 大学院医学研究院, 教授 (40361430)
折田 純久 千葉大学, 大学院医学研究院, 特任准教授 (60638310)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 多血小板血漿 / 凍結乾燥 / 成長因子 / platelet rich plasma / Platelet Rich Plasma / platelet-rich plasma / pharmacological action / bone-forming material |
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| Outline of Final Research Achievements |
Phamacological activities of platelet rich plasma: PRP and freeze-dried: fd PRP were evaluated. ELIZA tests of PDGF, FGF, and EGF showed similar concentrations in fresh PRP and 52 weeks saved PRP. Lumbar posterolateral fusion models were allocated to the sham, autologous graft, artificial bone-alone, artificial bone+fdRPR, artificial bone+PRP. Histological examination revealed similar good results in artificial bone+fdPRP and artificial bone+PRP.
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| Academic Significance and Societal Importance of the Research Achievements |
Platelet Rich Plasma: PRPと凍結乾燥したfreeze-dried:fd PRP（52週間保存後）の薬理活性はほぼ同程度だった。骨形成機能もBMPと同程度の効果があった。骨欠損のある患者で凍結乾燥したPRPが使用できれば臨床の現場で非常に有効である。今後は3-5年の長期保存の効果を明らかにするとともに、凍結乾燥PRPバンクの構築が実用化に向けて重要であると考える。
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction2020
Author(s)
Kinoshita H, Orita S, Inage K, Fujimoto K, Shiga Y, Abe K, Inoue M, Norimoto M, Umimura T, Ishii T, Yonemoto T, Kamoda H, Tsukanishi T, Akazawa T, Ohtori S
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Journal Title
Asian Spine J.
Volume: 14(1)
Issue: 1
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Freeze-Dried Platelet-Rich Plasma Induces Osteoblast Proliferation via Platelet-Derived Growth Factor Receptor-Mediated Signal Transduction.2020
Author(s)
Kinoshita H, Orita S, Inage K, Fujimoto K, Shiga Y, Abe K, Inoue M, Norimoto M, Umimura T, Ishii T, Yonemoto T, Kamoda H, Tsukanishi T, Suzuki M, Hirosawa N, Akazawa T, Ohtori S.
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Journal Title
Asian Spine J.
Volume: Jan 14(1)
Pages: 1-8
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Skeletal Muscle Cell Oxidative Stress as a Possible Therapeutic Target in a Denervation-Induced Experimental Sarcopenic Model2019
Author(s)
Kinoshita H, Orita S, Inage K, Yamauchi K, Abe K, Inoue M, Norimoto M, Umimura T, Eguchi Y, Fujimoto K, Shiga Y, Kanamoto H, Aoki Y, Furuya T, Suzuki M, Akazawa T, Takahashi K, Ohtori S.
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Journal Title
Spine
Volume: 44
Issue: 8
Pages: 446-455
DOI
Related Report
Peer Reviewed
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[Journal Article] A comparative study of flat surface design and medial pivot design in posterior cruciate-retaining total knee arthroplasty: a matched pair cohort study of two years.2018
Author(s)
Nakamura J, Inoue T, Suguro T, Suzuki M, Sasho T, Hagiwara S, Akagi R, Orita S, Inage K, Akazawa T, Ohtori S
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Journal Title
BMC Musculoskelet Disord.
Volume: 19
Pages: 234-241
Related Report
Peer Reviewed
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