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Elucidation of the regulatory mechanism of HPV gene expression by host transcriptional cofactors and development of anti-HPV peptide drugs.

Research Project

Project/Area Number 18K09244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56040:Obstetrics and gynecology-related
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Mori Seiichiro  国立感染症研究所, 病原体ゲノム解析研究センター, 主任研究官 (80342898)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsパピローマウイルス / 遺伝子発現 / 転写因子 / 転写共役因子 / 子宮頸がん / ペプチド薬
Outline of Final Research Achievements

The TEAD family of transcription factors requires associating cofactors to induce gene expression. TEAD1 is known to activate the early promoter of human papillomavirus (HPV), but the precise mechanisms of TEAD1-mediated transactivation of the HPV promoter, including its relevant cofactors, remain unexplored. In this study, we revealed that VGLL1, a TEAD-interacting cofactor, contributes to HPV early gene expression. Knockdown of VGLL1 and/or TEAD1 led to a decrease in viral early gene expression in human cervical keratinocytes and cervical cancer cell lines. We identified 11 TEAD1 target sites in the HPV16 long control region (LCR). VGLL1 bound to the HPV16 LCR via its interaction with TEAD1 both in vitro and in vivo. These results suggest that multiple VGLL1/TEAD1 complexes are recruited to the LCR to support the efficient transcription of HPV early genes.

Academic Significance and Societal Importance of the Research Achievements

HPVの遺伝子発現に関わる多くの宿主転写因子が報告されているが、転写共役因子についてはほとんど知られていない。本研究で、転写共役因子VGLL1が、転写因子TEAD1を介してHPVゲノムの転写調節領域に結合し、転写を活性化することが明らかとなった。TEAD1が広範な細胞種で発現しているのに対し、VGLL1は主に上皮細胞で発現していることから、VGLL1はHPVの上皮細胞への指向性に関わる重要な宿主因子と推測される。また、shRNAを用いてVGLL1をノックダウンすると子宮頸がん細胞の増殖が抑制されたことから、VGLL1は子宮頸がん治療の標的分子となり得る。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Transcription Factor Homeobox D9 Drives the Malignant Phenotype of HPV18-Positive Cervical Cancer Cells via Binding to the Viral Early Promoter2021

    • Author(s)
      Hayashi S, Iwata T, Imagawa R, Sugawara M, Chen G, Tanimoto S, Sugawara Y, Tanaka I, Matsui T, Nishio H, Nakamura M, Katoh Y, Mori S, Kukimoto I, Aoki D.
    • Journal Title

      Cancers (Basel)

      Volume: 13(18) Issue: 18 Pages: 4613-4613

    • DOI

      10.3390/cancers13184613

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Transcriptional Cofactor VGLL1 Drives Transcription of Human Papillomavirus Early Genes via TEAD12020

    • Author(s)
      Mori S, Takeuchi T, Ishii Y, Kukimoto I.
    • Journal Title

      J Virol

      Volume: 94 Issue: 10

    • DOI

      10.1128/jvi.01945-19

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia2020

    • Author(s)
      Baba S, Taguchi A, Kawata A, Hara K, Eguchi S, Mori M, Adachi K, Mori S, Iwata T, Mitsuhashi A, Maeda D, Komatsu A, Nagamatsu T, Oda K, Kukimoto I, Osuga Y, Fujii T, Kawana K.
    • Journal Title

      Virol J

      Volume: 17 Issue: 1 Pages: 32-32

    • DOI

      10.1186/s12985-020-01306-0

    • NAID

      120007089054

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions.2019

    • Author(s)
      Hirose Y, Onuki M, Tenjimbayashi Y, Yamaguchi-Naka M, Mori S, Tasaka N, Satoh T, Morisada T, Iwata T, Kiyono T, Mimura T, Sekizawa A, Matsumoto K, Kukimoto I.
    • Journal Title

      Viruses

      Volume: 11 Issue: 4 Pages: 350-350

    • DOI

      10.3390/v11040350

    • NAID

      120007133500

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-wide association study of cervical cancer suggests a role for ARRDC3 gene in human papillomavirus infection2019

    • Author(s)
      Takeuchi F, Kukimoto I, Li Z, Li S, Li N, Hu Z, Takahashi A, Inoue S, Yokoi S, Chen J, Hang D, Kuroda M, Matsuda F, Mizuno M, Mori S, Wu P, Tanaka N, Matsuo K, Kamatani Y, Kubo M, Ma D, Shi Y.
    • Journal Title

      Hum Mol Genet.

      Volume: 28 Issue: 2 Pages: 341-348

    • DOI

      10.1093/hmg/ddy390

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] TRANSCRIPTIONAL FACTOR TEAD1 AND ITS COFACTOR VGLL1 ARE REQUIRED FOR TRANSCRIPTION OF HPV16 EARLY GENES2019

    • Author(s)
      Mori S, Kukimoto I.
    • Organizer
      第67回日本ウイルス学会学術総会.
    • Related Report
      2019 Research-status Report
  • [Presentation] 転写共役因子VGLL1による転写因子TEADを介したヒトパピローマウイルス初期遺伝子の転写2018

    • Author(s)
      森 清一郎、竹内 隆正、石井 克幸、柊元 巌
    • Organizer
      第66回日本ウイルス学会学術総会.
    • Related Report
      2018 Research-status Report
  • [Presentation] TRANSCRIPTIONAL COFACTOR VGLL1 IS REQUIRED FOR TEAD-MEDIATED TRANSCRIPTION OF HPV EARLY GENES2018

    • Author(s)
      Mori S, Takeuchi T, Ishii Y, Kukikmoto I.
    • Organizer
      30th International Papillomavirus Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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