The vaginal microbiome in pathogenesis of endometriosis and endometriosis-related ovarian cancer

• Project/Area Number: 18K09274
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Tokai University
• Principal Investigator: SHIDA Masako 東海大学, 医学部, 講師 (10338717)
• Co-Investigator(Kenkyū-buntansha): 三上 幹男 東海大学, 医学部, 教授 (30190606) ; 平澤 猛 東海大学, 医学部, 准教授 (70307289) ; 今西 規 東海大学, 医学部, 教授 (80270461)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 子宮内膜症 / 卵巣癌 / 明細胞癌 / 膣細菌叢 / メタゲノム解析 / 明細胞腺癌 / 腟細菌叢 / 内膜症性嚢胞

Outline of Final Research Achievements

We hypothesized the impact of vaginal microbiome in pathogenesis of endometriosis and endometriosis-related ovarian cancer in young women because the pathogenesis of endometriosis is menstrual blood reflux into abdominal cavity which causes the change of its immunological environment, and sexual debut in more younger generations than before might cause the change in the vaginal microbiome. We analyzed 119 patient’s vaginal microbiome by SGS, and PCA analysis showed the different patterns of vaginal micirobiome between healthy women and patients of ovarian cancer, and endometriosis.

Academic Significance and Societal Importance of the Research Achievements

本研究結果をもとに内膜症・卵巣明細胞癌の発症に関連する細菌群を同定することで、発癌リスクマーカーとしての意義だけでなく、胃がんでのピロリ菌除去のように新しい予防治療法開発の可能性が想定される。本研究は、日本人に多く、若年者に発症し、かつ予後の悪い卵巣明細胞癌を、その前駆病変の子宮内膜症の発症を絡めた発想で立案した内容であり、少子化などの重要な国家的問題に対する解決策の一つになる可能性を秘める。

Research Products

• [Journal Article] Comprehensive Serum Glycopeptide Spectra Analysis Combined with Artificial Intelligence (CSGSA-AI) to Diagnose Early-Stage Ovarian Cancer. (2020)
  • Author(s): Tanabe K, Ikeda M, Hayashi M, Matsuo K, Yasaka M, Machida H, Shida M, Katahira T, Imanishi T, Hirasawa T, Sato K, Yoshida H, Mikami M.
  • Journal Title: Cancers (Basel) Volume: 12（9） Issue: 9 Pages: 2373-2373
  • DOI: 10.3390/cancers12092373
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epidemiological guideline influence on the therapeutic trend and patient outcome of uterine cervical cancer in Japan: Japan society of gynecologic oncology guideline evaluation committee project. (2020)
  • Author(s): Shigeta S, Shida M, Nagase S, Ikeda M, Takahashi F, Shibata T, Yamagami W, Katabuchi H, Yaegashi N, Aoki D, Mikami M
  • Journal Title: Gynecol Oncol Volume: 159 Issue: 1 Pages: 248-255
  • DOI: 10.1016/j.ygyno.2020.07.023
  • Peer Reviewed / Open Access
• [Journal Article] Utility of Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA) for the Detection of Early Stage Epithelial Ovarian Cancer. (2020)
  • Author(s): Matsuo K, Tanabe K, Hayashi M, Ikeda M, Yasaka M, Machida H, Shida M, Sato K, Yoshida H, Hirasawa T, Imanishi T, Mikami M.
  • Journal Title: Cancers (Basel) Volume: 12(9) Issue: 9 Pages: 2374-2374
  • DOI: 10.3390/cancers12092374
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Usefulness of laparoscopic restaging surgery for patients diagnosed with apparent early ovarian/fallopian tubal cancer by a prior surgery, a case control observational study in a single institute in Japan. (2020)
  • Author(s): Yoshida H, Yamamoto M, Shigeta H, Yasaka M, Machida H, Ikeda M, Shida M, Hirasawa T, Mikami M.
  • Journal Title: Eur J Gynaecol Oncol Volume: 41(6) Issue: 6 Pages: 960-968
  • DOI: 10.31083/j.ejgo.2020.06.2224
  • Peer Reviewed / Open Access
• [Journal Article] Recurrence, death, and secondary malignancy after ovarian conservation for young women with early-stage low-grade endometrial cancer. (2019)
  • Author(s): Matsuo K, Cripe JC, Kurnit KC,Mikami M,SHida M,et al.
  • Journal Title: Gynecol Oncol Volume: 155(1) Issue: 1 Pages: 39-50
  • DOI: 10.1016/j.ygyno.2019.08.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA): A Potential New Tool for Early Detection of Ovarian Cancer. (2019)
  • Author(s): Hayashi M, Matsuo K, Tanabe K, Ikeda M, Miyazawa M, Yasaka M, Machida H, Shida M, Imanishi T, Grubbs BH, Hirasawa T, Mikami M.
  • Journal Title: Cancers (Basel) Volume: 11(5) Issue: 5 Pages: 591-591
  • DOI: 10.3390/cancers11050591
  • NAID: 130007556094
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tumor characteristics and outcome of uterine carcinosarcoma in women aged 80 years. (2019)
  • Author(s): Matsuo K, Ross MS, Yunokawa M, Shida M,Mikami M,et al.
  • Journal Title: Surg Oncol Volume: 29 Pages: 25-32
  • DOI: 10.1016/j.suronc.2019.02.002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association of statins, aspirin, and venous thromboembolism in women with endometrial cancer (2019)
  • Author(s): Matsuo Koji、Hom Marianne S.、Yabuno Akira、Shida Masako、Kakuda Mamoru、Adachi Sosuke、Mandelbaum Rachel S.、Ueda Yutaka、Hasegawa Kosei、Enomoto Takayuki、Mikami Mikio、Roman Lynda D.
  • Journal Title: Gynecologic Oncology Volume: 152 Issue: 3 Pages: 605-611
  • DOI: 10.1016/j.ygyno.2018.12.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterizing sarcoma dominance pattern in uterine carcinosarcoma: Homologous versus heterologous element (2018)
  • Author(s): Matsuo Koji、Takazawa Yutaka、Ross Malcolm S.、Elishaev Esther、Yunokawa Mayu、Sheridan Todd B.、Bush Stephen H.、Klobocista Merieme M.、Blake Erin A.、Takano Tadao、Baba Tsukasa、Satoh Shinya、Shida Masako、Ikeda Yuji、et al.
  • Journal Title: Surgical Oncology Volume: 27 Issue: 3 Pages: 433-440
  • DOI: 10.1016/j.suronc.2018.05.017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clinical utility of CA-125 in the management of uterine carcinosarcoma (2018)
  • Author(s): Matsuo Koji、Ross Malcolm S.、Yunokawa Mayu、Johnson Marian S.、Machida Hiroko、Omatsu Kohei、Klobocista Merieme M.、Im Dwight D.、Satoh Shinya、Baba Tsukasa、Ikeda Yuji、Bush Stephen H.、Hasegawa Kosei、Blake Erin A.、Takekuma Munetaka、Shida Masako、Nishimura Masato、et al.
  • Journal Title: Journal of Gynecologic Oncology Volume: 29 Issue: 6
  • DOI: 10.3802/jgo.2018.29.e88
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Proposal for a Risk-Based Categorization of Uterine Carcinosarcoma (2018)
  • Author(s): Matsuo Koji、Takazawa Yutaka、Ross Malcolm S.、Elishaev Esther、Yunokawa Mayu、Sheridan Todd B.、Bush Stephen H.、Klobocista Merieme M.、Blake Erin A.、Takano Tadao、Baba Tsukasa、Satoh Shinya、Shida Masako、Ikeda Yuji、et al.
  • Journal Title: Annals of Surgical Oncology Volume: 25 Issue: 12 Pages: 3676-3684
  • DOI: 10.1245/s10434-018-6695-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Clinical Features of Recurrent Endometrial Cancer in Japan: Chemotherapy Instead of Radiotherapy as Postoperative Adjuvant Treatment (2018)
  • Author(s): Iwase Haruko、Furukawa Seigi、Hirasawa Takeshi、Asai Satoshi、Ohara Tatsuru、Hosonuma Shinji、Endo Shinichi、Tazo Yuki、Sato Hidetaka、Takada Toshio、Arai Masahide、Ikeda Masae、Shida Masako、Yoshioka Norihito、Tozawa-Ono Akiko、Suzuki Nao、Mikami Mikio、Onda Takashi
  • Journal Title: International Journal of Gynecologic Cancer Volume: 28 Issue: 8 Pages: 1616-1623
  • DOI: 10.1097/igc.0000000000001346
  • Peer Reviewed
• [Journal Article] Significance of Lymphovascular Space Invasion by the Sarcomatous Component in Uterine Carcinosarcoma (2018)
  • Author(s): Matsuo Koji、Takazawa Yutaka、Ross Malcolm S.、Elishaev Esther、Yunokawa Mayu、Sheridan Todd B.、Bush Stephen H.、Klobocista Merieme M.、Blake Erin A.、Takano Tadao、Baba Tsukasa、Satoh Shinya、Shida Masako、Ikeda Yuji、et al.
  • Journal Title: Annals of Surgical Oncology Volume: 25 Issue: 9 Pages: 2756-2766
  • DOI: 10.1245/s10434-018-6547-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Significance of abnormal peritoneal cytology on survival of women with stage I-II endometrioid endometrial cancer. (2018)
  • Author(s): Matsuo K, Yabuno A, Hom MS, Shida M, Kakuda M, Adachi S, Mandelbaum RS, Ueda Y, Hasegawa K, Enomoto T, Mikami M, Roman LD.
  • Journal Title: Gynecol Oncol Volume: 印刷中 Issue: 2 Pages: 301-309
  • DOI: 10.1016/j.ygyno.2018.02.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of institutional accreditation by the Japan Society of Gynecologic Oncology on the treatment and survival of women with cervical cancer (2018)
  • Author(s): Mikami Mikio、Shida Masako、Shibata Takeo、Katabuchi Hidetaka、Kigawa Junzo、Aoki Daisuke、Yaegashi Nobuo
  • Journal Title: Journal of Gynecologic Oncology Volume: 29 Issue: 2
  • DOI: 10.3802/jgo.2018.29.e23
  • Peer Reviewed / Open Access
• [Journal Article] Fully sialylated alpha-chain of complement 4-binding protein (A2160): a novel prognostic marker for epithelial ovarian carcinoma (2018)
  • Author(s): Matsuo Koji、Tanabe Kazuhiro、Ikeda Masae、Shibata Takeo、Kajiwara Hiroshi、Miyazawa Masaki、Miyazawa Mariko、Hayashi Masaru、Shida Masako、Hirasawa Takeshi、Roman Lynda D.、Mikami Mikio
  • Journal Title: Archives of Gynecology and Obstetrics Volume: 297 Issue: 3 Pages: 749-756
  • DOI: 10.1007/s00404-018-4658-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] UPLC-MS/MS based diagnostics for epithelial ovarian cancer using fully sialylated C4-binding protein (2018)
  • Author(s): Tanabe Kazuhiro、Matsuo Koji、Miyazawa Masaki、Hayashi Masaru、Ikeda Masae、Shida Masako、Hirasawa Takeshi、Sho Ryuichiro、Mikami Mikio
  • Journal Title: Biomedical Chromatography Volume: 32 Issue: 5
  • DOI: 10.1002/bmc.4180
  • Peer Reviewed
• [Journal Article] Significance of venous thromboembolism in women with uterine carcinosarcoma (2018)
  • Author(s): Matsuo Koji、Ross Malcolm S.、Im Dwight D.、Klobocista Merieme M.、Bush Stephen H.、Johnson Marian S.、Takano Tadao、Blake Erin A.、Ikeda Yuji、Nishimura Masato、Ueda Yutaka、Shida Masako、Hasegawa Kosei、et al.
  • Journal Title: Gynecologic Oncology Volume: 148 Issue: 2 Pages: 267-274
  • DOI: 10.1016/j.ygyno.2017.11.036
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 1.Early ovarian cancer detection by deep learning: Two-dimensional comprehensive serum glycopeptide spectra analysis. (2020)
  • Author(s): M Mikami, K Tanabe, K Matsuo, M Ikeda, M Hayashi, M Yasaka, H Machida, M SHida, T Hirasawa, T Imanishi.
  • Organizer: SGO 2020 Annual Meeting on Women's Cancer.
  • Int'l Joint Research
• [Presentation] Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA) might be a new (2018)
  • Author(s): M Ikeda, M Mikami, K Tanabe, K Matsuo, M Miyazawa, M Hayashi, H Machida,
  • Organizer: ASGO 5th International Workshop on Gynecologic Onclology
  • Int'l Joint Research
• [Presentation] Diagnosis of early stage ovarian clear cell carcinoma (CCC) and endometrioid carcinoma (EMC) - the combination assay of A2160 ( Alpha-chain of Complement 4-Binding Protein with Fully-Sialylated Glycans) (2018)
  • Author(s): M Hayashi, M Miyazawa, M Miyazawa, H Machida, M Ikeda, M Shida, THirasawa,
  • Organizer: ASGO 5th International Workshop on Gynecologic Onclology
  • Int'l Joint Research
• [Presentation] Predictors for Positive Surgical Margin of Cervical Intraepithelial Neoplasia Grade 3 After Therapeutic Cervical Conization: A Retrospective Multicenter Study (2018)
  • Author(s): M Yasaka, M Ikeda, M Shida, T Hirasawa, M Mikami, Y Kobayashi, S Nagase, M Yokoyama, T Enomoto, H Katabuchi
  • Organizer: 17th Biennial Meeting of the International Gynecologic Cancer Society ( IGCS2018 )
  • Int'l Joint Research
• [Presentation] Trends and Survival Outcomes of Women with Cervical Adenocarcinoma (2018)
  • Author(s): H Machida, I Oiwa, S Amatsu, T Narayama, T Iida, M Shida, T Hirasawa, M Mikami
  • Organizer: 17th Biennial Meeting of the International Gynecologic Cancer Society ( IGCS2018 )
  • Int'l Joint Research
• [Presentation] Prevention of Preterm Delivery After Cervical Conization for Cervical (2018)
  • Author(s): T Narayama, M Ikeda, M Shida, T Hirasawa, M Mikami, Y Kobayashi, S Nagase, M Yokoyama, T Enomoto, H Katabuchi
  • Organizer: 17th Biennial Meeting of the International Gynecologic Cancer Society ( IGCS2018 )
  • Int'l Joint Research