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Establishment of immunohistological method to improve the diagnostic accuracy of partial hydatidiform moles

Research Project

Project/Area Number 18K09281
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56040:Obstetrics and gynecology-related
Research InstitutionChiba University

Principal Investigator

Usui Hirokazu  千葉大学, 大学院医学研究院, 准教授 (90375634)

Co-Investigator(Kenkyū-buntansha) 佐藤 明日香  千葉大学, 医学部附属病院, 助教 (60779859)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords胞状奇胎 / 発現アレイ / 免疫染色 / マイクロアレイ / 発現マイクロアレイ / 定量的PCR法 / グロビン遺伝子 / 絨毛性疾患 / 遺伝子発現
Outline of Final Research Achievements

This study aimed to establish the immunohistochemistry that can more reliably differentiate between genetic partial moles and miscarriages. Genes helpful in distinguishing between partial moles and miscarriages were screened by expression microarray analysis of genetically diagnosed trophoblastic tissues. The two genes as HBE1 and HBZ were determined as the candidate genes. We have tried to generate primary antibodies (polyclonal antibodies) that can be used for immunostaining, but no specific antibodies have been raised to now.

Academic Significance and Societal Importance of the Research Achievements

胞状奇胎後には、侵入奇胎が発症することがあるため、術後に血清hCG測定による経過観察、血清hCG消失後に3~6カ月の避妊期間が必要である。部分胞状奇胎と両親由来二倍体との病理学的な鑑別が困難な場合がある。この場合、臨床的には、胞状奇胎として血清hCG測定・避妊などの管理を行うことになる。部分胞状奇胎と両親由来二倍体流産の鑑別が可能であれば、胞状奇胎ではない患者さんは、継続通院は不要になり、次回妊娠も早期に可能となる。このため、部分胞状奇胎と両親由来二倍体を区別する免疫染色法の確立は急務である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (45 results)

All 2021 2020 2019 2018

All Journal Article (17 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 17 results,  Open Access: 4 results) Presentation (27 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Spermatogonium-Derived Complete Hydatidiform Mole2021

    • Author(s)
      Usui Hirokazu、Shozu Makio
    • Journal Title

      New England Journal of Medicine

      Volume: 384 Issue: 10 Pages: 936-943

    • DOI

      10.1056/nejmoa2005756

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parental contribution to trisomy in heterozygous androgenetic complete moles2020

    • Author(s)
      Usui Hirokazu、Sato Asuka、Shozu Makio
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 17137-17137

    • DOI

      10.1038/s41598-020-74375-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Androgenetic Complete Hydatidiform Moles With p57KIP2-Positive Immunostaining2020

    • Author(s)
      Usui Hirokazu、Sato Asuka、Ota Masayuki、Ikeda Jun-ichiro、Shozu Makio
    • Journal Title

      American Journal of Clinical Pathology

      Volume: 154 Issue: 6 Pages: 776-783

    • DOI

      10.1093/ajcp/aqaa096

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABO blood type compatibility is not a risk factor for gestational?trophoblastic neoplasia development from androgenetic complete?hydatidiform moles2020

    • Author(s)
      Sato Asuka、Usui Hirokazu、Shozu Makio
    • Journal Title

      American Journal of Reproductive Immunology

      Volume: e13237 Issue: 6

    • DOI

      10.1111/aji.13237

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome-wide single nucleotide polymorphism array analysis unveils the origin of heterozygous androgenetic complete moles2019

    • Author(s)
      Usui Hirokazu、Nakabayashi Kazuhiko、Maehara Kayoko、Hata Kenichiro、Shozu Makio
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 12542-12542

    • DOI

      10.1038/s41598-019-49047-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Second Uterine Curettage and the Number of Chemotherapy Courses in Postmolar Gestational Trophoblastic Neoplasia2019

    • Author(s)
      Usui Hirokazu
    • Journal Title

      Obstetrics & Gynecology

      Volume: 134 Issue: 3 Pages: 649-649

    • DOI

      10.1097/aog.0000000000003441

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Radiation Therapy for Extrapelvic Lymph Node Recurrence After Curative Treatment for Cervical Cancer2019

    • Author(s)
      UNO TAKASHI、KANAZAWA AKI、NEMOTO MIHO WATANABE、HARADA RINTARO、KOBAYASHI HIROKI、SAITO MAKOTO、IWAI YUMA、USUI HIROKAZU、MITSUHASHI AKIRA、SHOZU MAKIO
    • Journal Title

      Anticancer Research

      Volume: 39 Issue: 2 Pages: 891-895

    • DOI

      10.21873/anticanres.13190

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison between vacuum aspiration and forceps plus blunt curettage for the evacuation of complete hydatidiform moles2019

    • Author(s)
      Sato Asuka、Usui Hirokazu、Shozu Makio
    • Journal Title

      Taiwanese Journal of Obstetrics and Gynecology

      Volume: 58 Issue: 5 Pages: 650-655

    • DOI

      10.1016/j.tjog.2019.07.012

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-term outcomes of progestin plus metformin as a fertility-sparing treatment for atypical endometrial hyperplasia and endometrial cancer patients2019

    • Author(s)
      Mitsuhashi Akira、Habu Yuji、Kobayashi Tatsuya、Kawarai Yoshimasa、Ishikawa Hiroshi、Usui Hirokazu、Shozu Makio
    • Journal Title

      Journal of Gynecologic Oncology

      Volume: 30 Issue: 6 Pages: 1-11

    • DOI

      10.3802/jgo.2019.30.e90

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial-Culture-Negative Subclinical Intra-Amniotic Infection Can Be Detected by Bacterial 16S Ribosomal-DNA–Amplifying Polymerase Chain Reaction2018

    • Author(s)
      Morimoto Sachi、Usui Hirokazu、Kobayashi Tatsuya、Katou Eiji、Goto Shunji、Tanaka Hirokazu、Shozu Makio
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 71 Issue: 4 Pages: 274-280

    • DOI

      10.7883/yoken.JJID.2017.468

    • NAID

      130007418122

    • ISSN
      1344-6304, 1884-2836
    • Year and Date
      2018-07-31
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Variant Prolactin Receptor in Agalactia and Hyperprolactinemia2018

    • Author(s)
      Kobayashi Tatsuya、Usui Hirokazu、Tanaka Hirokazu、Shozu Makio
    • Journal Title

      New England Journal of Medicine

      Volume: 379 Issue: 23 Pages: 2230-2236

    • DOI

      10.1056/nejmoa1805171

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Response to Letter to ‘Removal of retained products of conception showing marked vascularity without uterine artery embolization: Two case reports’2018

    • Author(s)
      Usui Hirokazu、Sato Asuka、Shozu Makio
    • Journal Title

      Journal of Obstetrics and Gynaecology Research

      Volume: 45 Issue: 2 Pages: 487-487

    • DOI

      10.1111/jog.13857

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Removal of retained products of conception showing marked vascularity without uterine artery embolization: Two case reports2018

    • Author(s)
      Usui Hirokazu、Sato Asuka、Okayama Jun、Suzuki Yoshiya、Omoto Akiko、Shozu Makio
    • Journal Title

      Journal of Obstetrics and Gynaecology Research

      Volume: 44 Issue: 8 Pages: 1482-1486

    • DOI

      10.1111/jog.13678

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gestational Trophoblastic Neoplasia From Genetically Confirmed Hydatidiform Moles: Prospective Observational Cohort Study2018

    • Author(s)
      Usui Hirokazu、Qu Jia、Sato Asuka、Pan Zijun、Mitsuhashi Akira、Matsui Hideo、Shozu Makio
    • Journal Title

      International Journal of Gynecologic Cancer

      Volume: 28 Issue: 9 Pages: 1772-1780

    • DOI

      10.1097/igc.0000000000001374

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Elucidation of the developmental mechanism of ovarian mature cystic teratomas using B allele-frequency plots of single nucleotide polymorphism array data2018

    • Author(s)
      Usui Hirokazu、Nakabayashi Kazuhiko、Kaku Hiroshi、Maehara Kayoko、Hata Kenichiro、Shozu Makio
    • Journal Title

      Genes, Chromosomes and Cancer

      Volume: 57 Issue: 8 Pages: 409-419

    • DOI

      10.1002/gcc.1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Treatment results of the second-line chemotherapy regimen for patients with low-risk gestational trophoblastic neoplasia treated with 5-day methotrexate and 5-day etoposide2018

    • Author(s)
      Kanno Toshiyuki、Matsui Hideo、Akizawa Yoshika、Usui Hirokazu、Shozu Makio
    • Journal Title

      Journal of Gynecologic Oncology

      Volume: 29 Issue: 6

    • DOI

      10.3802/jgo.2018.29.e89

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 胞状奇胎自然寛解後の妊娠予後2018

    • Author(s)
      佐藤 明日香, 碓井 宏和, 生水 真紀夫
    • Journal Title

      産婦人科の実際

      Volume: 67 Pages: 895-900

    • NAID

      40021663380

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] p57kip2免疫染色陽性であった雄核発生奇胎の2例2020

    • Author(s)
      碓井 宏和, 佐藤 明日香, 羽生 裕二, 松岡 歩, 錦見 恭子, 楯 真一, 三橋 暁, 生水 真紀夫
    • Organizer
      第72回日本産科婦人科学会学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Management of a patient with a hydatidiform mole with an unfavorable human chorionic gonadotropin course2020

    • Author(s)
      Sato Asuka, Usui Hirokazu, Shozu Makio
    • Organizer
      第72回日本産科婦人科学会学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 絨毛癌に対する救済化学療法の後方視的検討2020

    • Author(s)
      碓井宏和、佐藤明日香、羽生裕二、松岡歩、 錦見恭子、楯真一、三橋暁、生水真紀夫
    • Organizer
      第62回日本婦人科腫瘍学会学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 胞状奇胎自然寛解後の続発症発生率と奇胎後経過観察期間についての考察2020

    • Author(s)
      佐藤 明日香, 碓井 宏和, 生水 真紀夫
    • Organizer
      第62回日本婦人科腫瘍学会学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 絨毛性腫瘍治療後の妊娠予後2020

    • Author(s)
      碓井宏和、佐藤明日香、生水真紀夫
    • Organizer
      第38回絨毛性疾患研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 胞状奇胎1次管理中の選択的再掻爬の実際2019

    • Author(s)
      佐藤 明日香, 碓井 宏和, 生水 真紀夫
    • Organizer
      第37回日本絨毛性疾患研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] p57KIP2免疫染色陽性であった雄核発生奇胎の2例2019

    • Author(s)
      碓井 宏和、佐藤 明日香、羽生 裕二、松岡 歩、錦見 恭子、楯 真一、三橋 暁、生水 真紀夫
    • Organizer
      第37回日本絨毛性疾患研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Relationship between ABO blood groups of androgenetic complete hydatidiform moles and development into gestational trophoblastic neoplasia2019

    • Author(s)
      Asuka Sato, Hirokazu Usui, Zijun Pan, Yuji Habu, Ayumu Matsuoka Kyoko Nishikimi, Shinichi Tate, Akira Mitsuhashi, Makio Shozu
    • Organizer
      第20回世界絨毛性疾患会議
    • Related Report
      2019 Research-status Report
  • [Presentation] Two cases of androgenetic complete hydatidiform moles showing p57KIP2-positive immunostaining2019

    • Author(s)
      Hirokazu Usui, Asuka Sato, Makio Shozu
    • Organizer
      第20回世界絨毛性疾患会議
    • Related Report
      2019 Research-status Report
  • [Presentation] 胞状奇胎の掻爬回数と続発症頻度に関する多施設調査研究2019

    • Author(s)
      兼城英輔, 碓井宏和, 山本英子, 田代浩徳, 宮本新吾, 井箟一彦, 八重樫伸生, 榎本隆之
    • Organizer
      第61回日本婦人科腫瘍学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 絨毛癌に対するMEA療法の検討2019

    • Author(s)
      碓井 宏和, 佐藤 明日香, 羽生 裕二, 松岡 歩, 塙 真輔, 錦見 恭子, 楯 真一, 三橋 暁, 生水 真紀夫
    • Organizer
      第61回日本婦人科腫瘍学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 胞状奇胎手術後の血流豊富な子宮病変に対する子宮内容除去術についての検討2019

    • Author(s)
      佐藤 明日香, 碓井 宏和, 羽生 裕二, 松岡 歩, 塙 真輔, 錦見 恭子, 楯 真一, 三橋 暁, 生水 真紀夫
    • Organizer
      第61回日本婦人科腫瘍学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Laeverin is a possible marker of placental site trophoblastic tumor2019

    • Author(s)
      Hiroshi Fujiwara, Kyosuke Kagami, Ayumi Matsuoka, Masamori Ono, Akihito Horie, Sachiko Minamiguchi, Hirokazu Usui
    • Organizer
      71th Annual Congress of Japan Society of Obstetrics and Gynecology
    • Related Report
      2019 Research-status Report
  • [Presentation] Safety and efficacy of removal surgery for retained products of conception that exhibit hypervascularity, without concurrent uterine artery embolization2019

    • Author(s)
      Asuka Sato, Hirokazu Usui, Yoshiya Suzuki, Jun Okayama, Akiko Omoto, Makio Shozu
    • Organizer
      71th Annual Congress of Japan Society of Obstetrics and Gynecology
    • Related Report
      2019 Research-status Report
  • [Presentation] Exploration of specific markers of a partial hydatidiform mole2019

    • Author(s)
      Hirokazu Usui, Asuka Sato, Yuji Habu, Ayumu Matsuoka, Shinsuke Hanawa, Kyoko Nishikimi, Shinichi Tate, Akira Mitsuhashi, Makio Shozu
    • Organizer
      71th Annual Congress of Japan Society of Obstetrics and Gynecology
    • Related Report
      2019 Research-status Report
  • [Presentation] 絨毛性疾患に対するDNA診断とその応用2018

    • Author(s)
      碓井 宏和, 生水 真紀夫
    • Organizer
      第57回 日本臨床細胞学会秋季大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 思春期・小児期のヒト骨組織からのアロマターゼ活性の検出2018

    • Author(s)
      碓井 宏和, 西須 孝, 生水 真紀夫
    • Organizer
      第26回日本ステロイドホルモン学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Dilatation and evacuation for retained products of conception with hypervascularity without uterine artery embolization2018

    • Author(s)
      Asuka Sato, Hirokazu Usui, Jun Okayama, Akiko Omoto, Yoshiya Suzuki, Makio Shozu
    • Organizer
      International Federation of Placenta Associations 2018 Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Risk factors of post-molar gestational trophoblastic neoplasia: prospective observational cohort study2018

    • Author(s)
      Hirokazu Usui, Asuka Sato, Zijun Pan, Makio Shozu
    • Organizer
      International Federation of Placenta Associations 2018 Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] LAEVERIN AS A POSSIBLE MARKER OF PSTT2018

    • Author(s)
      Hiroshi Fujiwara, Kyosuke Kagami, Masamori Ono, Hirokazu Usui
    • Organizer
      International Federation of Placenta Associations 2018 Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Application of DNA polymorphisms for gestational trophoblastic disease2018

    • Author(s)
      Hirokazu Usui, Asuka Sato, Zijun Pan, Makio Shozu
    • Organizer
      International Federation of Placenta Associations 2018 Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] GESTATIONAL TROPHOBLASTIC NEOPLASIA FROM CYTOGENETIC HYDATIDIFORM MOLES: PROSPECTIVE OBSERVATIONAL COHORT STUDY2018

    • Author(s)
      Hirokazu Usui, Asuka Sato, Zijun Pan, Makio Shozu
    • Organizer
      17th Biennial Meeting of the International Gynecologic Cancer Society
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 中期中絶後に生じた子宮内血管病変からの一時的止血にバルン圧迫術が奏功した1例2018

    • Author(s)
      岡山潤, 尾本暁子, 佐藤明日香, 碓井宏和, 生水真紀夫
    • Organizer
      第54回日本周産期・新生児医学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 血流を伴う妊娠組織遺残に対する子宮内容除去術の治療成績2018

    • Author(s)
      佐藤 明日香, 碓井 宏和, 岡山 潤, 鈴木 義也, 尾本 暁子, 生水 真紀夫
    • Organizer
      第54回日本周産期・新生児医学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 血流著明な絨毛遺残に対して最大ラミナリア法を適用した1例:動脈塞栓術回避への戦略2018

    • Author(s)
      碓井宏和, 佐藤明日香, 岡山潤, 鈴木義也, 尾本暁子, 生水真紀夫
    • Organizer
      第54回日本周産期・新生児医学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Analysis of paternal contribution of genetic partial hydatidiform moles using subtraction B allele frequency plotting of SNP array2018

    • Author(s)
      Hirokazu Usui, Asuka Sato, Makio Shozu
    • Organizer
      70th Annual Congress of Japan Society of Obstetrics and Gynecology
    • Related Report
      2018 Research-status Report
  • [Presentation] Prognostic effect of ABO blood groups in patients with androgenetic complete hydatidiform moles2018

    • Author(s)
      Asuka Sato, Hirokazu Usui, Zijun Pan, Yuji Habu, Ayumu Matsuoka, Kyoko Nishikimi, Shinichi Tate, Akira Mitsuhashi, Makio Shozu
    • Organizer
      70th Annual Congress of Japan Society of Obstetrics and Gynecology
    • Related Report
      2018 Research-status Report
  • [Book] 基礎と臨床の両側面からみた胎盤学2019

    • Author(s)
      日本胎盤学会編、分担執筆 19絨毛性疾患 胞状奇胎の遺伝学的診断
    • Total Pages
      430
    • Publisher
      MEDICAL VIEW
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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