| Project/Area Number |
18K09304
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
桑原 慶充 日本医科大学, 医学部, 准教授 (40373013)
根岸 靖幸 日本医科大学, 医学部, 准教授 (50644580)
小野 修一 日本医科大学, 医学部, 講師 (80465301)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 中隔子宮 / 不育症 / 流産 / 樹状細胞 / ケモカイン / ケモカインレセプター / 子宮鏡下中隔切除術 / CD141+ 樹状細胞（DC） / RANTES / 着床不全 / 子宮形成術 / 樹状細胞遊走因子 / 子宮筋層 / NKT細胞 |
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| Outline of Final Research Achievements |
Septate uterus is associated with spontaneous abortion. However, immunological mechanisms for miscarriage in patients with septate uterus remain completely unknown. Immune cells, dendritic cells (DCs), macrophages, T cells, natural killer cells, invariant natural killer cells, and chemokine receptors in US and uterine myometrium tissue (UMT) were analyzed using flow cytometry and immunohistochemical staining. The percentage of CD141+ DCs in US was significantly lower than that in UMT. Both US and UMT showed CCR1 and CCR5 expression on CD141+ DCs; however, the production of chemokines, MIP-1α, RANTES, and MIP-3β was abundant in UMT-obtained viable cells. The accumulation of CD141+ DCs was lower in US than that in UMT. This phenomenon may be caused by low chemokine productions in US. Our findings support the benefit of surgical intervention for septate uterus-that is, the elimination of inappropriate implantation sites.
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| Academic Significance and Societal Importance of the Research Achievements |
中隔子宮は流産や不育症の原因になると言われているが、そのメカニズムは明らかになっていなかった。本研究では免疫学的なアプローチによりそのメカニズムの一端を解明することができた。
近年、適切な炎症は着床、妊娠初期に必要である事が報告されている。中隔子宮に対する手術療法の意義も明らかでなかったが、炎症性CD141+ DCsの欠如した中隔部を切除することは、本来起こるべき適切な炎症反応が起こらない部位を切除するという意味において、手術療法の妥当性を裏付けることになった。
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