| Project/Area Number |
18K09375
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | プロサポシン / GPR37 / GPR37L1 / 蝸牛 / 神経栄養因子 |
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| Outline of Final Research Achievements |
Prosaposin is a glycoprotein composed of approximately 520 amino acid residues. In the vestibuloauditory system, luck of prosaposin results in the deafness accompanying a severe morphological abnormality of the hair cell. Recently, G protein-coupled receptor (GPR) 37 and GPR37L1 are identified as candidates for prosaposin receptors. In this study, the expression patterns of prosaposin, GPR37 and GPR37L1 were examined in mouse cochlea. By reverse transcript-polymerase chain reaction, prosaposin, GPR37 and GPR37L1 mRNA were found in mouse cochlea cDNA. By immunofluorescence, both GPR37 and GPR37L1 immunoreactivity were observed in the inner hair cell and the outer phalangeal cell. These results indicate prosaposin may affect not only to the inner hair cell but also to the outer phalangeal cell.
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| Academic Significance and Societal Importance of the Research Achievements |
プロサポシンは中枢神経系において神経栄養因子として機能しており、プロサポシンの投与により、虚血による神経細胞の遅発性細胞死の抑制効果や、神経細胞再生の促進効果が報告されている。本研究により、蝸牛の恒常性に関わるプロサポシンの機能的意義の一端が明らかになり、またその受容体の発現性が明らかになったことから、外傷性聴覚障害治療へのプロサポシンの応用を考える上での基礎的貢献を果たすことができたと考えられる。
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